Screening microsatellite markers from EST sequences of bay scallop Argopecten irradians

Seventy-five simple sequence repeats （SSRs） were identified by the bioinformatic analysis from 5008 expressed sequence tags （ESTs） of Argopecten irradians. Among the SSRs, the number of repeat nucleotide varied from 2 to 6. Dinucleotide and trinueleotide repeat motifs were dominant in EST-SSRs of bay scallop, with a proportion of 80% over the total screened SSRs. Twenty-nine pairs of primer were designed based on the flank sequences of the selected ESTs using the software of Primet 5, and verified under the given PCR reaction condition. Eighteen of the 29 primer pairs resulted in the expected products, while the remaining either failed to produce any fragments or yielded products over expected size. Thirteen of the 18 SSRs, accounting for 72%, were detected to show polymorphism in the examined scallop samples. A preliminary test in this study indicated that the majority of the identified SSRs were informative in the cultured bay scallops, making them suitable for the population and other genetic analysis. EST-SSR markers have more advantages than the traditional genomic-derived SSRs and there is a wide range of application in comparative mapping, functional gene cloning and marker assisted selection. This research provides a reference to the identification of EST-SSRs with relative bioinformatic analysis from aquaculture species, as well as to those with a large number of ESTs.     

面向Web应用的前置URL控制机制研究

针对反向代理应用中存在着的网页内嵌URL问题,提出了前置URL控制机制,构建了该机制的总体模型,包括URL定向模块、预分析器、HTML/XML解析器、URL修改模块和COOKIE修改模块各部件.论述了模型的核心技术--动态脚本的HTML解析和XSL/XML联合解析,提出了异地代理的解决方案.在Apache/Linux下实现了该模型原型,对其功能进行了完整测试,包括性能负载测试,测试结果表明该机制能高效地完成预期目标.     

Bioinformatic analysis of expressed sequence tags from sporophyte of Porphyra yezoensis （Bagiaceae, Rhodophyta）

A total of 719 expressed sequence tags (EST) clustered into 329 non?redundant EST groups are obtained from the sporophyte cDNA library of red algae, Porphyra yezoensis. Gene Ontology (GO) analysis is employed in characterizing 60 strictest annotated unique genes out of the 329 EST groups and some domains such as COX1, Sod-Fe-C, GST-N, SHMT, and RNase-PH related to the enzymes and proteins functioning in cells have been identified by HMMPFAM search. As its leafy gametophyte, the similar codon usage with strong bias is found in P. yezoensis filamentous sporophyte, regardless of some differences found in given amino acids. The average GC content of the 329 unique genes is 53.0%. In contrast, the third nucleotide of codon exhibits a higher GC content (72%) than that of the first (58%) and the second (42%) nucleotides. Similarity search of the present study shows a novel EST ratio of 60.2%, which is against the Porphyra ESTs database, suggesting further investigations towards elucidating the characteristics of Porphyra functional genome.     

Sequence analysis of mRNA polyadenylation signals of rice genes

Most eukaryotic mRNAs receive a poly (A) tail at their 3′-ends through a process involving the cleavage of pre-mRNA and the concomitant polymerization of adenosine residues to the cleaved RNA end[1,2]. As un- translated regions (UTRs) may contain importa…     

用Web技术制作电子教案研究

研究采用ＨＴＭＬ,结合动画设计,ＡＳＰ和数据库编程技术制作一个比较完整,内容覆盖教学各方面的电子教案的过程及相关技术,为ＭＩＳ及其它课程的多媒体教学提供一种新的思路。     

XML原理与应用

介绍了XML的定义特点。结合搜索引擎讨论了XML的应用。最后分析了XML的未来发展。     

ASP技术考

ASP(ActiveServerPage)是微软开发的一项新技术.文章阐述了ASP的工作过程和运行环境,并探讨了其通过ADO操纵后台数据库的方法.     

跨平台可视化网页设计器的设计与实现

设计基于Java的跨平台可视化网页设计器。使用最新的Java开发工具JDK1．2,根据当前的输入信息,完成网页设计器应具有的各项功能。该网页设计器汇聚了目前流行的各种网页设计器的优点,同时具有其独特的特性－－跨平台,可使用在多种操作系统上,实现了一个功能齐全的网页设计器。     

苔藓分子生物学的一些进展

本文简述了近年来苔藓分子生物学的突破性进展。 1995年以前 ,苔藓系统学中尚无DNA序列数据 ,但到 1999年产生了 2 5 0个分类单位的 10 0 0个DNA序列 ,并分析了它们的系统发生信息。在分子遗传学研究中 ,小立碗藓Physcomitrellapatens、葫芦藓Funariahygrometrica、土生墙藓Tortularuralis和角齿藓Cer atodonpurpureus正在发展成有力的工具。表达序列靶 (ESTs)和外源基因的表达已成功地用于这些苔藓植物。同源重组在酵母和小白鼠细胞中已经确定 ,然而至今尚无任何植物模型体系。 1997年Schaefer和Zryd证明 ,小立碗藓具有有效的同源重组能力 ,至今在所有植物中是独特的 ,这在分析植物基因功能中将显示是十分有用的     

《计算机文化基础》远程考试系统的开发

介绍了用WWW技术实现《计算机文化基础》远程考试系统的开发 .