G蛋白偶联受体激酶5在大肠杆菌中的表达纯化

通过RT-PCR扩增得到了人G蛋白偶联受体激酶5(GProtein-coupledreceptorki-nase,GPK5)的基因,将其克隆到表达载体pET-28a中,并将该质粒转入大肠杆菌BL21(DE3),在10μmol/L异丙基硫代-β-D-半乳糖苷(IPIG)诱导培养下表达,通过分离纯化,得到部分纯化的GRK5蛋白。体外活性测定表明,纯化后的GPK5能够特异性地磷酸化视紫红质。动力学常数Km=4.3μmol/L,Vmax=25nmol/(mg·min),与文献报道的从真核表达系统中纯化得到的GRK5相近。     

球丸裹包机电气控制设计

介绍了ＢＮ１２型球丸裹包机电气自动控制电路的构成及工作原理，叙述了工作中瞬间出现无丸等工况下的自动控制过程。     

AMS／5近交系小鼠培育及其生物学特性的研究

近交纯系动物具有一致的基因型和遣传特征，个体之间差异极小，实验中容易取得一致的结果＾「１．２」。随着医学科学研究的飞速发展，近交系动物的培育及应用愈来愈被人们所重视，为适应不同课题研究需要而培育的近交系动物的品系越来越多。由于各种原因，国内开展这项工作较晚，长期以来我们缺少更多合格的近交动物品系来进行大量生产和供应。我院于１９７４年３月从封闭群ＬＡＣＡ小鼠中选留亲本，进行严格的近亲同胞兄妹交配，宗     

Identification and biological significance of 4-methyl-3Z,5-hexadienoic acid produced by males of the gall-forming tephritidsUrophora cardui (L.) andUrophora stylata (Fab.) (Diptera: Tephritidae)

Summary A new natural substance has been identified in the rectal ampullae of gall-forming fruit flies. The substance was found to be the only volatile compound in the rectal ampulla of maleUrophora cardui andUrophora stylata. GC-MS methods were used to characterize its structure as 4-methyl-3Z,5-hexadienoic acid. Physiological parameters such as the amount of the acid at different ages and under different conditions were investigated. The biological significance of the new volatile as an arresting pheromone was tested in several bioassays. The arrestant function could not be established, but the results gave hints of a territorial function between conspecific males. The results are discussed with respect to gland morphology and predictions of communication models among fruit flies.     

Self-Converse Score Lists in Oriented Graphs

Ｓｅｌｆ－ＣｏｎｖｅｒｓｅＳｃｏｒｅＬｉｓｔｓｉｎＯｒｉｅｎｔｅｄＧｒａｐｈｓ￥ＰｉｒｚａｄａＳｈａｒｉｅｆｕｄｄｉｎ（ＤｅｐａｒｔｍｅｎｔｏｆＡｐｐｌｉｅｄＭａｔｈｅｍａｔｉｃｓＦａｃｕｌｔｙｏｆＥｎｇｇ．ａｎｄＴｅｃｈ．Ａ．Ｍ．Ｕ．，Ａｌｉｇａｒ...     

Chemical defence in the larvae of the leaf beetleGonioctena viminalis L. (Coleoptera: Chrysomelidae)

Summary The leaf beetle larva ofGonioctena (Phytodecta) viminalis L. has been shown to produce five volatile constituents within its paired abdominal defensive gland reservoirs. It is the first time that these compounds have been reported to occur in coleopteran defensive glands (linalool, phenylethanol) and Chrysomelidae larvae (6-methyl-5-hepten-2-one, 6-methyl-5-hepten-2-ol, 2-hexenal). In addition to the gross morphology of theGonioctena gland and its discharge behavior, the natural products found are discussed in terms of chemotaxonomy.     

MPEG-2多节目的系统复用分析

本文主要研究了MPEG - 2多节目系统复用的问题。对多路节目的复用 ,在图像通信领域 ,可采用两种编码方式 :独立编码和联合编码。在独立编码中介绍了帧平移算法和基于宏块条的互相关算法 ;对于联合编码 ,本文介绍了一种利用超帧概念的基于率失真理论的联合码率控制策略 ,同时阐述TM 5码率控制原理。最后得出结论 ,独立编码中重建图像存在显著差异 ,联合码率控制可以改善此缺陷 ,可节省频带资源 ,但仍需要进一步研究。     

广义弹簧质量系统模态分析的有限元解法

本文针对广义弹簧质量系统模态分析的特点,将有限元素法引入其求解过程,从而可以充分发挥通用有限元分析程序的作用,实例表明,方法是行之有效的。     

5-取代丙二酸亚异丙酯构象的量子化学研究

5-取代丙二酸亚异丙酯衍生物构象目前还有争议,我们用半经验分子轨道的MNDO方法对其三种有代表意义的分子进行了量子化学计算,得出一些有意义的结果。确定5-(甲基)丙二酸亚异丙酯的稳定构象为椅式;5-(甲酰甲基)丙二酸亚异丙酯的稳定构象为船式;5-(肟基)丙二酸亚异丙酯的稳定构象为信封式,并对不同构象的成因作了探讨。