2-氨基-3-氰基-4-(2’,4’-二氯苯基)-4H-萘并[1,2-b]吡喃的合成和晶体结构

标题化合物C22H17Cl2NO3的晶体由α-乙氧羰基-2,4-二氯肉桂腈、β-萘酚在TEBA存在下合成并经DMF与水的混合溶剂重结晶而得.其结构通过元素分析、IR和1HNMR进行了表征,用X射线单晶衍射法测定了该化合物的晶体结构.结果表明,晶体属于单斜晶系,P21/n空间群。a=1.450 5(2)nm,b=0.903 7(1)nm,c=1.491 3(2)nm,α=90°,β=101.21(1)°,γ=90°,Mr=414.27,V=1.917 5(4)nm3,Dc=1.435 g·cm-3,Z=4,μ(MoKα)=0.362 mm-1,F(000)=856.晶体结构用直接法解出,经全矩阵最小二乘法修正,最终偏离因子R=0.044 44,Rw=0.117 7.化合物分子中,原子C(1),C(2),C(3),C(4),C(5)和O(1)形成一个六元环,该六元环中C(1)-C(2)键长为0.134 6(3)nm,表明为双键.此六元环为船式构象.     

Cloning and functional analysis of chloroplast division gene NtFtsZ2-1 in Nicotiana tabacum

FtsZ protein plays an important role in the division of chloroplasts. With the finding and functional analysis of higher plant FtsZ proteins, people have deepened the understanding in the molecular mechanism of chloroplast division. Multiple ftsZ genes are diversified into two families in higher plants, ftsZ1 and ftsZ2. On the basis of the research on ftsZ1 family, we analyzed the function of NtFtsZ2-1 gene in Nicotiana tabacum. Microscopic analysis of the sense and antisense NtFtsZ2-1 transgenic tobacco plants revealed that the chloroplasts were abnormal in size and also in number when compared with wild-type tobacco chloroplasts. Our investigations confirmed that the NtFtsZ2-1 gene is involved in plant chloroplast division.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

尕斯油田N_2~1油藏南区储层非均质性研究

针对尕斯油田N21油藏南区储层非均质性严重的特点,从层间、层内及平面非均质性3个方面进行了系统研究.层间非均质性相对较弱,层间隔层分布较为稳定,各油组砂层发育情况差别不大.层内非均质性较强,并且与沉积微相密切相关.单砂层垂向上渗透率的变化以正韵律和复合韵律模式为主,局部发育反韵律模式.按照非均质性的强弱,沉积微相依次为:分流河道、河口砂坝、分流间湾、远砂坝.统计了平面上不同微相砂体的几何形状,并且采用流动单元法综合评价储层的平面展布特征.     

1,3-苯二氧基二乙酸二芳酯的合成及植物生长调节活性

以PEG 4 0 0为催化剂 ,在液 液相转移催化条件下合成了一系列新的 1,3 苯二氧基二乙酸二芳酯 .用元素分析、IR以及1 HNMR确定了它们的结构 .初步生物活性试验表明 ,该类化合物对小麦幼苗的生长有一定的促进作用 .     

铜离子在4-甲氧基-2,6-二(3,5-二甲基吡唑)-1,3,5-三嗪吸附型修饰电极上的电化学行为

用循环伏安法和方波伏安法研究了铜离子在4 甲氧基 2,6 二(3,5 二甲基吡唑) 1,3,5 三嗪(简称MBDT)吸附型修饰电极上的电化学行为.通过吸附在玻碳表面的配体与铜离子的配位作用,对Cu(Ⅱ)和Cu(Ⅰ)进行了电分析,结果表明,Cu(Ⅰ)在修饰电极上表现出比Cu(Ⅱ)高得多的灵敏度.     

(3+1)维NNV方程的精确解

引入一个简单的变换，把(3 1)维Nizhnik-Novikov-Vesdov(NNV)方程化为一维KdV方程，从而通过已知KdV方程的解得到(3 1)维NNV方程的若干精确解。这种方法可以推广开来，方便地建立起某一高维方程和其它低维非线性方程的联系，然后通过求解低维的非线性方程找到高维非线性方程的精确解。     

1,3-二芳基-2-甲基-2-碘甲基-1,3-丙二酮的合成

以1,3 二芳基 1,3 丙二酮为原料,经过二次烷基化反应合成了1,3 二芳基 2 甲基 2 碘甲基 1,3 丙二酮.产物结构经红外光谱、核磁共振光谱和质谱得到了证实.     

PTC法以苯为原料电化学氧化合成对苯醌的研究

报道以苯为原料，H2SO4，NaSO4为支持电解质，采用PTC法，在铅基二氧化铅阳极上电化学氧化合成对苯醌的方法和合适工艺条件的研究。实验结果表明这是一条很有潜力的工艺路线。