临床完全缓解卵巢上皮性癌CA125界值与预后相关性研究

探讨了临床完全缓解卵巢上皮性癌患者CA125界值与复发的相关性,它关系着卵巢癌患者的预后,可为减少难治性卵巢癌的发生提供参考.     

人食管癌癌旁粘膜培养上皮生长特性的研究

人食管癌癌旁组织12例和正常食管粘膜15例组织块在盖玻上用199培养基培养,并掺入放射性氘胸腺嘧啶核苷.用光镜、显微分光光度计,放射自显影,扫描电镜检查移植块生长上皮层的生长速度,细胞核 DNA 含量,DNA 合成和形态学改变。结果是前三周,癌旁粘膜组织块培养上皮的生长率,细胞 DNA 含量和 DNA 合成高于食管正常粘膜.第四周以后两组上皮出现生长率降低,分化增加现象,在此培养中未能见到癌旁粘膜上皮转化为恶性细胞.本实验正常粘膜的培养,其生长的上皮细胞可以作为食管癌致癌因素研究的模型.     

EC109细胞株细胞周期DNA代谢和形态改变的研究

人食管癌细胞株 EC109以显微分光光度计测定癌细胞核 DNA 含量,以放射自显影测定细胞核摄取~3H-TdR 和扫描电镜检查细胞表面的超微结构.大多数 EC109瘤细胞间期细胞核的 DNA 含量增加,主要干系细胞 DNA 含量为2 C,属 G_1期,也可见低于四倍体(3 C),高于四倍体(>4 C).甚至多倍体(>8 C)的瘤细胞.~3H-TdR 加入培养基10分钟后,许多瘤细胞核有银粒出现(S 期细胞).大量细胞进入细胞周期反映其恶性程度高.扫描电镜可见 EC109在细胞周期不同阶段其细胞大小形状和微绒毛也不同.本文结果说明用细胞核 DAN 含量测定,~3H-TdR 掺入和细胞表面超微结构可测定肿瘤周期.也可用这些方法判定瘤细胞的恶性程度.     

S 100A8 mediates the activation of P65/HLA- B/S 100A8/BCL-2/Caspase-9 （-3） pathway in laryngeal carcinogenesis

S100 calcium binding protein A8 （S100A8）, a possible novel member of NF-kappa B signal pathway in laryngeal squamous cell carcinoma （LSCC）, interacts with human leukocyte antigen B （HLA-B） which carries an NF-kappa B binding site within the enhancer A. The objective of this study was to explore the molecular mechanism of S100A8 in laryngeal carcinogenesis. RT-PCR, Western blotting and immunohistochemistry staining were applied to evaluate the expression levels of IKKα, P65, REL-B, S100A8, APAF-1 and BCL-2 genes. The signal transduction passway in which S100A8 might participate was explored by RNA interference. Flow cytometry, TUNEL assay and cell invasion in vitro were used to detect the biological behavior of Hep2 cells induced by S100A8 gene. Our results showed that high expression of S100A8 was related to tumorigenesis in LSCC and negatively correlated with the degree of differentiation, indicating that S100A8 gene could inhibit apoptosis and promote metastasis in LSCC. Additionally, the suppression of S100A8 by RNA interference down-regulated BCL-2 but not APAF-1, P65 and IKKα, while, the suppression of P65 could significantly down-regulate the expression of S100A8 gene. In conclusion, S100A8 plays an important role in P65/HLA-B/S100A8/BCL-2/Caspase-9 （-3） pathway in laryngeal carcinoma.     

乳头状肾细胞癌的临床病理研究(附11例报道)

为研究乳头状肾癌的临床病理特征、鉴别诊断与预后,对11例乳头状肾癌进行形态学观察、免疫组化染色与TNM分期,并随访患者。结果发现:11例乳头状肾癌镜下以乳头状、乳头-管状结构为主,45.4%(5/11)为1型乳头状肾癌,乳头表面被覆小细胞,胞质稀少,单层排列;54.5%(6/11)为2型乳头状肾癌,肿瘤细胞核分级高,胞质嗜酸性,假复层排列。免疫组化,11例乳头状肾癌AMACR、CK、CD10、CD117、Vim、MDM2的表达率分别为100%、72.7%、36.4%、36.4%、36.4%,0%,1型乳头状肾癌CK7的阳性率为100%,2型乳头状肾癌中Topoisomerase IIα为66.6%,P53阳性率为50%。乳头状肾癌具有特殊的临床病理特征,是一类独立的疾病单元,AMACR、CK7、TopIIα表达有助于乳头状肾癌及亚型的诊断,CK7、TopIIα与P53的表达可能与预后相关。     

Effects of retinoic acid on ascites cells of the TA3 mouse mammary carcinoma

Summary Retinoic acid caused a decrease in adhesiveness but no growth change in the allotransplantable TA3-Ha cell and no change in adhesiveness or growth in the strain specific TA3-St cell. The retinoic acid binding protein was detected in the TA3-Ha, but not the TA3-St, cell.This study was supported in pat by Public Health Service Grants CA-08418 and CA-18600 from the National Cancer Institute, National Institutes of Health. This is publication 955 of the Robert W. Lovett Group for the Study of Diseases Causing Deformities. We gratefully acknowledge the contributions of Drs Luigi M. De Luca and Anton M. Jetten of the Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institutes of Health, Bethesda, MD, without whose help this study would not have been possible.     

Immunocytochemical and enzymatic detection of lysozyme in human colon carcinoma cell lines

Summary Six of a total of 14 human colon carcinoma cell lines produce and secrete lysozyme in vitro. Three also produce the enzyme when propagated in vivo in athymic mice. None of the lysozyme positive cells stained in a manner typical of Paneth cells. Additionally, lysozymes from all six colon lines possess identical molecular weights (14,000 daltons).This work was supported by funds from the Monsanto Company under agreements with Harvard University. We are indebted to A. Ehrlich, S. Kane, and D. Sleeman for excellent technical assistance and to Dr B. Vallee for continued advice and support.J.W.F. and E.M.A. are also members of the Department of Pathology, Harvard Medical School.     

用自身血清抗体筛选人肝细胞肝癌抗原

为了从分子水平上筛选肝细胞肝癌抗原，应用作者所在实验室创建的“重组克隆表达抗原的血清学鉴定技术”（ＳＥＲＥＸｓｅｒｏｌｏｇｉｃａｌｉｎｄｅｎｔｉｆｉｃａｔｉｏｎｏｆａｎｔｉｇｅｎｓｂｙｒｅｃｏｍｂｉｎａｎｔｅｘｐｒｅｓｉｏｎｃｌｏｎｉｎｇ）筛选人肝细胞肝癌（ＨＣＣ）组织构建的ｃＤＮＡ文库，发现了许多与病人，自身血清高滴度抗体反应的克隆。应用多种异体（其他ＨＣＣ病人，多种非ＨＣＣ肿瘤病人，Ｂ型、Ｃ型病毒性肝炎病人，肝硬化病人以及健康人）血清反应分析表明，高滴度抗体反应多数出现在ＨＣＣ病人。参照基因库寻找这些克隆的同源结构，发现其中许多抗原为至今未知基因所编码。进一步的血清和抗原分子分析将为ＨＣＣ特异免疫治疗和诊断提供基础资料     

92例甲状腺单发结节的超声鉴别诊断

目的:探讨二维及彩色多普勒超声在甲状腺单发结节鉴别诊断中的价值.方法:回顾分析经手术及病理证实的92例甲状腺单发结节,共分3组,其中甲状腺腺瘤组16例,结节性甲状腺肿组59例,甲状腺癌组17例.比较以上3组的二维及彩色多普勒声像图并与相应病理对照分析.结果:[1]在二维声像图上:对3组病例的甲状腺形状、边界、周边回声、晕环、囊变、钙化及颈部淋巴结肿大进行比较,其差异均有显著性意义(P＜0.05).[2]在彩色多普勒血流成像(CDFI)显示上:对3组病例的结节内部及周边血流的分布进行比较,其差异均有显著性(P＜0.05).[3]超声诊断甲状腺腺瘤的敏感度为100%,特异度为64.5%;诊断结节性甲状腺肿的敏感度为52.5%,特异度为100%;诊断甲状腺癌的敏感度为82.4%,特异度为94.7%.结论:病灶微小钙化和颈部淋巴结肿大的存在提示恶性后果,囊变的存在提示良性结果,二维及彩色多普勒超声对甲状腺腺瘤、单发性结节性甲状腺肿、甲状腺癌的鉴别诊断有帮助.但是单发性结节性甲状腺肿较易误诊为甲状腺腺瘤.