基于PIC的双鉴式火灾监测报警系统设计

以PIC单片机为主控芯片,利用温度传感器和烟雾传感器,对指定的监测场所进行实时火灾安防监控．温度传感器和烟雾传感器分别对火灾过程中所产生的较高温度和大量烟雾进行监测,从而发出相应的报警信号．本课题研究,两种火灾探测传感器,组成的双鉴式火灾报警系统．可以将其中安装墙上,从而及时、准确无误地预测火灾险情．     

p16与原发性肝细胞癌的研究进展

p16基因又称肿瘤抑制基因(MTS1),是近年来发现的一个重要的抑癌基因,在细胞周期中发挥重要的调节作用.现对p16基因的结构、p16蛋白的作用途径以及该基因突变与原发性肝细胞癌的关系进行综述.     

Isolation and Characterization of a New Subspecies of Aeromonas salmonicida （ Aeromonas salmonicida subsp, flounderacida subsp. nov. ） from Stone Flounder（ Kareius bicoloratus L. ）

Biological properties were studied to appropriate pathogenic bacteria which were isolated from diseased (or dead) stone flounder (Kareius bicoloratus L. ) which expressed bacterial septicaemia, including morphological characteristics, colony characteristics, physiological and biochemical characteristics and serum homology of isolates, the results showed that the isolates belonged to a new subspecies of A. salmonicida. In addition, the representative strains have been re-checked and detected the mol% G C ratio of the DNA by China Center for Type Culture Collection (CCTCC), the examined strains were also regarded as a new subspecies of A. salmonicida, and designated as Aeromonas salmonicida subsp, flounderac/da subsp, nov. by its isolated fish (Kareius bicoloratus ). Molecular identification of analysis of the nucleotide sequence of the 16S rRNA gene were applied, the results showed high similarity (99%) with the 16S rRNA gene of Aeromonas salmonicida from GenBank database. Cluster analysis of phylogenetic tree revealed that the representative strain formed separately bootstrap-supported cluster.     

Comparison of the community structure of planktonic bacteria in ballast water from entry ships and local sea water in Xiamen Port

In this study, the bacterial community structures in samples of ballast water collected from a ship from Singapore and of local sea water collected from Xiamen Port were compared using restriction fragment length polymorphism (RFLP) and 16S rDNA sequence analysis. Except for dominant α-Proteobacteria that are common to both systems, the bacterial community structures of the two systems were quite different. Most of the clones derived from the different systems were grouped into different phylogenetic clusters, and the sys-tems share only one common RFLP pattern. The ballast water, which is likely from clean offshore waters, contains sequences specific to α- and γ-Proteobacteria. Phylogenetic analysis revealed that the ballast water contained sequences belonging to attached bacteria and bacteria commonly found in the open sea, as well as many novel sequences. In addition, no known pathogenic bacteria were detected in the ballast water samples. Conversely, water samples from Xiamen Port were apparently affected by the near shore environments.Specifically, in addition to α- and γ-Proteobacteria, water from Xiamen Port contained β- and δ-Proteobacteria, Synechococcus, Bacter-oidetes and Actinobacteria, which are common in coastal environments. Additionally, four pathogenic bacterial sequences and one plas-mid sequence of a potential red tide forming alga were detected in the water from Xiamen Port, which suggests that the local sea water is polluted. The results of this study can be used as background information to assess the risk associated with the introduction of non-indig-enous species to local systems and to establish ballast water management systems.     

Genetic diversity of rhizobial populations recovered from three Lotus species cultivated in the infra-arid Tunisian soils

Eighty-three bacterial strains isolated from root nodules of Lotus creticus, L. pusillus, and L. arabicus grown in infra-arid Tunisian soils were characterized using a polyphasic approach including phenotypic analysis, rep-PCR and PCR-RFLP analyses of the 16S rRNA gene. Phenotypically, all isolates are fast growers the majority of which grow at a pH of between 5.5 and 9. Most of the tested isolates tolerate NaCl concentrations from 1.39% to 3.48%. By rep-PCR fingerprinting, the genomic similarity varied from 30% to 98%. All tested isolates were clustered into 32 rep-PCR clusters at the similarity level of 80%. The genomic divergence of strains revealed by rep/PCR analysis appeared to be very important since a molecular polymorphism delimiting symbionts for each species of Lotus was identified. With the high-resolution of rep-PCR profiles of the isolates obtained using Pearson’s/UPGMA analysis, the isolates were resolved into 60 different profiling groups to undergo 16S ARDRA analyses. The analysis of all restriction fragments from each strain based on the UPGMA algorithm from the combined patterns showed that Lotus isolates are very diverse and that they were affiliated to Sinorhizobium, Rhizobium, and Mesorhizobium genera.     

尖塘鳢属鱼类线粒体16SrRNA基因序列变异及分子系统进化

为探讨尖塘鳢属鱼类的系统发育关系,笔者通过PCR法得到云斑尖塘鳢、线纹尖塘鳢和尖头塘鳢的线粒体16SrRNA基因部分序列（569bp）,并结合从GenBank中下载的平头沙塘鳢、溪鳢及刺盖塘鳢的16SrRNA基因部分同源序列,分析了6种塘鳢科鱼类的系统发育关系．运用MAGA4．0软件分析可知,塘鳢鱼类6个种之间存在142个变异位点,其中简约信息位点57个,转换／颠换之比为1．6,表明塘鳢科鱼类16SrRNA基因序列转换／颠换未达饱和;6种塘鳢科鱼类之间的序列差异在0．054～0．147之间,其中平头沙塘鳢和刺盖塘鳢的序列差异最大（0．147）,线纹尖塘鳢与云斑尖塘鳢的序列差异最小（0．054）,且种间序列差异不大．通过NJ,ME,MP系统树的建立,表明线纹尖塘鳢与云斑尖塘鳢的亲缘关系最近,且尖塘鳢属与塘鳢属鱼类的同源性不高,验证了传统分类学把尖塘鳢独立成属的科学性．     

对虾养殖池沉积物细菌的遗传多样性

为了解对虾养殖池沉积物中细菌的遗传多样性,采用细菌通用引物构建了细菌16S rRNA基因克隆文库,并从文库中随机挑选克隆子测序,得到37条有效序列,进行序列同源性分析和系统进化分析.结果表明：对虾养殖池底泥中细菌的遗传多样性非常丰富.主要分为六大类群：其中γ-变形菌纲细菌占据优势地位,约占所分析克隆子数的41% 其次是α-变形菌纲和δ-变形菌纲细菌,分别占19% 还有少量的酸杆菌纲、放线菌门和厚壁菌门细菌,分别占所分析克隆子数的3%-8%.所得克隆子序列大都与GenBank库中来自土壤环境中的未培养克隆子序列相似,并且大部分序列的相似性值小于95%,说明对虾养殖池中的细菌大都是未培养的未知细菌,特异地存在于土壤环境中.     

西藏大学2008级新生心理素质普查报告

此次普查采用症状自评量表、卡特尔16PF人格问卷、人际信任量表三种工具,对西藏大学2008级新生1850人的心理健康状况进行了测查。得出结论：（1）6％的大学生需要进一步检查;（2）女大学生在心理健康总分和除敌对之外的各维度上的得分极其显著地高于男大学生的得分;（3）在恐怖和精神病性两个维度上不同民族大学生存在显著差异;（4）在精神病性维度上不同出生地大学生存在显著差异;（5）不同性别和不同民族的大学生在人格方面存在显著差异;（6）不同性别和不同民族的大学生在人际信任方面存在显著差异。     

乌江网箱养殖丁(魚歲)细菌的分离与分子鉴定

利用微生物学常用的细菌分离、纯化方法从丁(魚歲)中分离出9株菌株,编号分别为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ、Ⅷ、Ⅸ.分别扩增出9株菌株的16S rRNA基因约600 bp的片段,并对其测序,将测序结果输入到NCBI(http://www.ncbi.nlm.nih.gov/)中,用BLAST工具对序列进行同源性比对分析,并利用MEGA4绘制系统进化树,结果表明:9株菌株的16S rRNA基因测序结果分别与不动杆菌(Acinetobacter)、嗜水气单胞菌(Aeromonas hydrophi-la)、希瓦氏菌(Shewanella baltica)、不动杆菌(Acinetobacter)、希瓦氏菌(Shewanella baltica)、霍乱弧菌(Vibriocholerae)、枯草芽孢杆菌(Bacillus subtilis)、杀鲑气单胞菌(Aeromonas salmonicida)、肺炎克雷伯氏菌(Klebsiellapneumoniae)的16S rRNA基因的核苷酸序列同源,同源性分别为96%,99%,99%,97%,98%,98%,99%,98%,98%.9株菌株的系统进化树明显分为3支,Ⅰ和Ⅳ聚为一支,Ⅶ独聚一支,Ⅱ、Ⅲ、Ⅴ、Ⅵ、Ⅷ、Ⅸ聚为一支,其中Ⅱ和Ⅷ相聚,Ⅲ和Ⅴ相聚后再与Ⅵ相聚,最后与Ⅸ相聚.     

新疆汉族乳腺癌及乳腺良性病变组织中HPV16E6的半巢式PCR检测

了解新疆地区汉族乳腺癌及乳腺良性病变组织中人类乳头状瘤病毒(Human papillomavirus,HPV)感染的情况,探讨乳腺癌及乳腺良性病变是否存在HPV16感染及HPV16感染与乳腺癌发病之间的关系.采用半巢式PCR(semi-nested polymerase chain reaction)技术检测80例乳腺癌及60例乳腺良性病变组织中HPV16感染的情况.结果 80例乳腺癌病例中,有22例(27.5%)检测到HPV16的DNA片断;60例乳腺良性病变中,有2例(3.3%)检测到HPV16的DNA序列,检测结果作χ2检验,χ2=14.10,P<0.001,差异有统计学意义.新疆汉族乳腺癌及乳腺良性病变存在HPV16感染,HPV16在乳腺癌的发病中发挥着一定的作用.