高迁移组染色体蛋白-17的分离提取及其抗体ELISA测定方法的建立

纯化高迁移组染色体蛋白－１７（ＨＭＧ－１７）,建立检测抗ＨＭＧ－１７自身抗体的ＥＬＩＳＡ方法,探讨抗ＨＭＧ－１７抗体与临床疾病的关系。用水煮沸法提取,甲酸－吡啶缓冲液酸化和ＳｅｐｈａｄｅｘＧ－１５０凝胶过滤技术,自小牛胸腺组织中分离ＨＭＧ－１７,用此纯化物包被,方阵滴定,建立测定抗ＨＭＧ－１７抗体的间接ＥＬＩＳＡ方法,并进行方法学考核和临床标本检测。纯化的ＨＭＧ－１７抗原经十二烷基硫酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）分析,呈现一条分子量约９２００的蛋白带;建立的ＥＬＩＳＡ方法批内平均变异系数（ＣＶ）为５．８％,批间ＣＶ值为９．６％,ＥＬＩＳＡ抑制试验最高抑制率达８７．２％;临床检测结果表明,在系统性红斑狼疮（ＳＬＥ）、混合性结缔组织病（ＭＣＴＤ）和干燥综合征（ＳＳ）患者中分别有１７．４％～３７．３％的抗ＨＭＧ－１７抗体的阳性检出率（Ｐ＜０．０１或Ｐ＜０．０５）,而在其他自身免疫病和内科疾病者中,抗ＨＭＧ－１７抗体均为低水平。抗ＨＭＧ－１７抗体ＥＬＩＳＡ测定法具有较好的特异性和重复性,便于常规检测,该类抗体的存在似与某些自身免疫病（尤其是ＳＬＥ）的关系较为密切     

Molecular mechanism of actomyosin-based motility

Sophisticated molecular genetic, biochemical and biophysical studies have been used to probe the molecular mechanism of actomyosin-based motility. Recent solution measurements, high-resolution structures of recombinant myosin motor domains, and lower resolution structures of the complex formed by filamentous actin and the myosin motor domain provide detailed insights into the mechanism of chemomechanical coupling in the actomyosin system. They show how small conformational changes are amplified by a lever-arm mechanism to a working stroke of several nanometres, explain the mechanism that governs the directionality of actin-based movement, and reveal a communication pathway between the nucleotide binding pocket and the actin-binding region that explains the reciprocal relationship between actin and nucleotide affinity. Here we focus on the interacting elements in the actomyosin system and the communication pathways in the myosin motor domain that respond to actin binding.Received 12 January 2005; received after revision 4 March 2005; accepted 23 March 2005     

教师教学档案管理软件系统的开发

笔者开发了适于我院实际情况的教师教学档案管理软件,该软件功能较强、界面友好、保密性强、易于操作,应用该软件实现了教师教学档案管理的计算机化.     

Novel and sensitive noncompetitive enzyme immunoassay for kassinin in rat plasma

Summary A novel and sensitive noncompetitive enzyme immunoassay for kassinin is described. Kassinin was biotinylated using sulfosuccinimidyl-6-(biotinamido)hexanoate. The biotinylated kassinin was trapped on antikassinin IgG-coated polystyrene balls and, after washing to eliminate other biotinylated substances, was eluted with HCl. The biotinylated kassinin eluted was reacted with anti-kassinin Fab'-peroxidase conjugate and trapped onto streptavidin-coated polystyrene balls. Peroxidase activity bound to the polystyrene balls was assayed by fluorimetry. The detection limit of kassinin was 0.13 pg (0.1 fmol)/tube or 0.065 g/l of rat plasma, which was 750-fold or 15-fold lower than that for competitive radioimmunoassay.     

Effect of enzyme and mechanical stirring on the degradation of polyaspartic acid hydro-gel

As a biodegradable polymer, polyaspartic acid(PASP) hydro-gel has been applied in various fields. Although the degradation mechanism of PASP was well studied, to our knowledge, limited research has been done on the degradation of PASP hydro-gel, whose main chain was crosslinked. This work mainly focused on the effects of enzyme and mechanical stirring on the structure of the PASP hydro-gel during its degradation.The change of the molecular weight and swelling ratio indicates that the simple mechanical stirring caused a break at the crosslink point of the PASP hydro-gel's spatial reticular structure with IR and SEM characterization, but has no significant effect on the degradation of the main chain.Changing the PH value of the degradation system also has little effect on the degradation of the main chain. However, the additions of enzyme both resulted in the break at the crosslink point and the degradation of the main chain, the significant decreases of both the molecular weight and swelling ratio of the PASP hydro-gel were observed.     

SO2动态熏气对地衣的急性毒作用研究

以淡黄腹梅衣Xanthoparmeliamexicana（Gyeln）Pale为研究对象，以叶绿素、蛋白质、超氧化物歧化酶（SO）、过氧化物酶（POD）、还原型谷光甘肽（GSH）、酸性磷酸酶、硝酸还原酶、甘露醇脱氨酶（MDH）、葡萄糖-6-磷酸脱氢酶（G6PDH）、海藻糖酶为生理生化指标，运用动态熏气和生态毒理学方法，研究了特定条件（温度25℃、相对湿度（60±2）％、光强900～1000lx）下，一定浓度范围SO2动态熏气对地衣组成及代谢的影响。结果表明：随着熏气浓度的增加，叶绿素脱镁比率及含量降低，蛋白质合成受损；SOD、POD活性加强，GSH相对含量降低，酸性磷酸酶活性波式上升，MDH、G6PDH及海藻糖酶活性加强。     

胞间通道的形成机理与物质运输

衰老器官内有机物质转移和重新利用，依赖于胞间连丝的运输和调节，为了适应衰老器官内大量的降解物质在短期内迅速转移到贮藏器官和生长部位，胞间连丝内部的压缩内质网在酶的作用下降解成简单的通道，其周围的细胞壁物质也被酶解，使胞间加丝通道拓宽，并且在没有胞间连丝的细胞壁上，也能形成新的通道，孔径拓宽的胞间连丝无论是在结构上，还是在功能上与胞间连丝发生时的起始状态小孔道十分类似，还提出了胞间连丝随生命体周期性     

广播活动的策划与广播形象的塑造

文章论述了广播活动策划的概念、广播活动策划的作用及广播活动策划的注意事项,以具体生动的实际策划案例简述了广播策划活动在塑造广播形象中的重要作用。     

县级图书馆读者活动的策划与组织

文章强调县级图书馆开展读者活动的重要性,分析了影响县级图书馆开展读者活动的因素,提出了县级图书馆策划和组织读者活动的方法以及应注意的问题。     

酶工程双语教学实践探讨

双语教学是中国教育改革的一项全新尝试。在生物工程专业学生中进行酶工程双语教学,是提高教学质量,和提高学生专业外语水平及应用能力的有效方法。通过双语教学的现状和效果等方面的分析,探讨了酶工程双语教学中存在的问题,提出了改进酶工程双语教学的思路和方法。