中专学生"厌学"现象分析及对策

针对目前中专学生“厌学”的普遍现象，分析了影响中专学生“厌学”的主要原因，提出了中专学生只有改变观念，克服“厌学”情绪，才能激发学习的积极性。     

谈高职院校外籍教师的聘请与管理

高等职业院校聘请外籍教师工作有其特殊性.广开聘请渠道、把好聘请关,是高职院校聘外工作的关键;依法管理、科学管理、人本管理是提高聘用效益的核心.     

Expression and Characterization of HGV E2 cDNA in Pichia pastoris

A 559 base pair fragment of cDNA locating at the putative E2 region of GBV-C/HGV was in-serted into Pichia pastoris expression vector pPICgK in the reading frame of α-factor secreting signal pep-tide. The recombinant expression plasmid pPIC9K-E2 was introduced into P. pastoris GSll5 with electro-poration and recombined with the host genome by homological recombination. The His+Mut+ recombinantyeasts were selected and cultivated in the BMMY medium. After 3 days induction with 0. 5% methanol,the target protein(E2) accumulated up to 30% of total proteins in the supernatant. The expressed E2 pro-tein was proved possessing antigenicity and high specificity with Western blot and ELISA probed with serafrom the immunized rabbits and the patients infected by GBV-C/HGV.     

英语新课标下教师角色的转变

施行新的《英语课程标准》,要求教师做好充分的心理准备并实现必要的角色转变:教师应该是学生学习的促进者;应该是与学生“平等中的首席”;应该是谦虚的学习者;教师之间应该是紧密的合作者;教师应该做扎实的研究者。     

对我国电子竞技运动发展现状的思考

我国电子竞技运动的发展目前还处于初级阶段,没有统一固定的专业组织,没有规范有序的竞赛市场,广大电子竞技爱好者的运动水平难以获得大的提高. 要想改变现状,跟上世界电子竞技运动的发展步伐,我们必须创新理念. 首先,获得政府的支持和观众的广泛参与,争取企业的赞助,制定政策,完善赛制,使电子竞技迈上发展的正常轨道;其次,走职业化、产业化道路,探索产业赢利模式,培育和开发市场;第三,博采众长,形成中国电子竞技运动的特色,打造自己的品牌,促进整个信息产业和体育事业的发展.     

Fecundity of the blood-feeding insectRhodnius prolixus increases in successive periods of egg production

The fecundity of the blood-feeding insect,Rhodnius prolixus, was observed to increase in successive periods of egg production, each period being triggered by a single large blood meal. As previously published, the fecundity of mated animals was significantly higher than that of unmated animals for the first period of egg production. For a second period of egg production, fecundity increased significantly in both mated and unmated animals. By the fourth period, fecundity had returned to first-feed values for mated animals, but remained high for unmated animals, and the fecundity of mated and unmated animals was not significantly different. Thus, during successive periods of egg production, the processes which maintain fecundity of unmated animals below that of mated animals are overcome.     

重组大肠杆菌利用醋酸及乳酸为碳源合成PHB

以廉价的工业副产物醋酸及乳酸为碳源,对产PHB重组大肠杆菌进行培养,考察醋酸及乳酸的添加对重组大肠杆菌生长及PHB产量的影响。将来自Cupriavidusnecator的PHB合成操纵子phaCAB基因簇克隆至pBAD载体,得到产PHB菌株BL21＿pBAD＿phaCAB,以阿拉伯糖为诱导剂,在大肠杆菌中进行重组表达。分别使用LB及M9培养基,对重组菌株BL21＿pBAD＿phaCAB进行培养,研究其生长速度及PHB产量,探索产PHB重组大肠杆菌最适培养基。以添加0.04 g/L乳酸、1.2 g/L乳酸、0.02 g/L醋酸、0.6 g/L醋酸、0.04 g/L乳酸+0.02g/L醋酸、1.2g/L乳酸+0.4g/L醋酸的M9培养基(均含2 g/L葡萄糖)为实验组,以M9培养基(含2g/L葡萄糖)为对照组,考察醋酸及乳酸的添加对重组大肠杆菌生长及PHB产量的影响。分别取第6,12,24和36小时的培养液,分析其葡萄糖、醋酸及乳酸含量的变化。结果表明,低氮型M9培养基更适合产PHB重组大肠杆菌在低糖培养环境中生长。在葡萄糖消耗殆尽后,大肠杆菌能够以醋酸及乳酸为碳源进行代谢,因此在培养基中...     

改进的细菌转化试验方法

本文对“细菌转化实验方法”进行了改进。用改良碱酶法制备的质粒DNA(PBR322)作供体,转化经氯化钙处理的受体菌E.Coli C600,使后者获得抗氨苄青霉素和抗四环素的特性。改进后的转化方法操作简便,不受设备限制。     

仔猪混合感染致病性白色葡萄球菌和大肠杆菌的诊断及防制初报

经过病原的分离鉴定、致病性试验及病仔猪防治效果的分析,确诊某集约化养猪场仔猪发生以“渗出性皮炎”为主的疾病为致病性白色葡萄球菌和大肠杆菌混合感染,通过采取综合的防制措施后,彻底控制本病的发生和流行。     

几种金属材料拉伸断裂断口磁性的电子显微研究

利用电子显微技术对几种金属材料拉伸断裂断口进行了分析,对它们的力学性能、拉伸磁性、断面微观结构进行了比较研究,得出了它们之间存在的一些特性,并从理论上作了一些初步的探讨。给出了铁磁体ΔＥ效应的应用。