关于t—半群与广义E—极小半群

引进强分裂元以及广义Ｅ－极小半群的概念，从而给出半群Ｓ为ｔ－半群的充要条件是：具有ＰＩＥＰ或无强分裂元或具有某种ＣＥＰ，若周期半群Ｊ是广义Ｅ－极小半群，则Ｓ是一些ｐ群的并或一个左（或）零半群（或２个元素的半格）或诣零半群或幂零半群。     

基于Pro/Engineer平台齿轮数控加工程序自动生成的实现

基于Pro／E平台，利用可视化语言VC 6．0的集成环境，对Pro／E的加工模块进行二次开发，实现了针对齿轮数控加工交互界面的可视化设计及CAM系统工艺信息的自动化获取、数控代码的自动生成。     

转录辅因子CBP/p300提高转录因子C/EBP介导的人白细胞介素5(IL-5)基因启动子活性

IL-5主要由Th2类细胞产生, 在过敏性哮喘等过敏性疾病的发生过程中起着关键的作用. 转录辅因子CBP/p300本身具有组蛋白乙酰转移酶活性, 参与许多基因的转录调控过程. 腺病毒E1A癌蛋白能与CBP/p300蛋白结合并抑制其活性. 我们分析了E1A蛋白在IL-5基因启动子/荧光素酶报告基因表达调控中的作用, 结果表明E1A蛋白能抑制PMA/离子霉素激活和转录因子C/EBPβ介导的IL-5基因启动子/荧光素酶报告基因的活性. 而突变体E1AΔ2-36蛋白因不能与CBP/p300蛋白结合而不能抑制IL-5基因启动子/荧光素酶报告基因的表达. 此结果揭示, 组蛋白乙酰转移酶CBP/p300参与IL-5基因启动子活性的调控. 另外, 转录辅因子CBP/p300和转录因子C/EBPβ可以协同地激活IL-5基因启动子/荧光素酶报告基因的表达. 为进一步研究IL-5基因表达调控的机制奠定了基础.     

大肠杆菌中重组猪生长激素提取方法及变复性的比较研究

通过对基因工程菌pgh／E．coli BL21用酶溶解、超声波处理、超声波处理与液氮冻融相结合三种方法进行破菌，用盐酸胍法进行变复性，提取重组猪生长激素(rpGH)．结果表明用超声波处理与液氮冻融相结合的方法裂解细菌，盐酸胍法变复性，rpGH的提取率最高，达72．16％；而用尿素法对rpGH在大肠杆菌内所形成的包涵体变复性，rpGH的提取率仅为49．82％．     

环境质量现状评价系统的数据库设计

从实际开发项目出发，详细阐述了基于关系模型的数据库系统的设计思想及实现方法，尤其对于动态表格的处理做了较为详细的论述，同时还以微软的VFP6．0为开发平台介绍了数据库的具体设计．     

基于Petri网下的哲学家就餐问题的模拟与验证

哲学家就餐问题可以看作当应用程序中包含并发线程的执行时,处理共享资源合作的一个有代表性的问题,该问题是评价同步方法的一个测试标准.利用Petri 网的C/E系统来模拟哲学家就餐问题具有很好的实用背景,因为C/E系统下的条件事件集的发生可以用开关或者门电路来实现.通过采用C/E系统来完善哲学家就餐问题的模型,并给出一个可以允许外部条件来加以有选择控制的模型.     

致密湖相碳酸盐岩油气富集模式及稳产、高产主控因素——以柴达木盆地英西地区为例

基于岩心观察和系统的微观岩石学和结构学特征研究,探究了英西地区渐新统的储集层特征、油气富集模式及稳产、高产主控因素:(1)目的层岩性以湖相碳酸盐岩为主,可分为盐间和盐下两套地层,盐间广泛发育厚盐层,以石盐为主,盐下零星发育薄盐层,以硬石膏为主;(2)咸化条件下沉积的这套细粒的湖相碳酸盐岩,既是品质最好的烃源岩,又发育白云岩"甜点"储集层,具有自生自储特征(上部盐层因具有很高的毛管突破压力和塑性特征而具有很强的封盖能力,且盐下应力集中造缝),其多因素的良好匹配形成了盐下油气规模富集的有利区;(3)基质晶间孔的发育程度控制着油气藏的规模和稳产,微裂缝的发育程度控制着油气藏的富集和高产。这一研究成果对深入研究英西E_3~2盐下油气的分布规律和下一步勘探部署具有重要的指导意义。     

DanHong Injection inhibits the development of primary abdominal aortic aneurysms in apoE knockout mice

Clinical observations indicate that DanHong Injection （DHI） can increase blood flow and reduce various syndromes in patients with cardiovascular disease. How- ever, it still needs to define the function of DHI and the involved mechanisms in details, such as the protective effect on the development of primary abdominal aortic aneurysms （AAAs）. In this study, we determined whether DHI is able to inhibit AAA in apoE knockout （apoE-/-） mice. Thirty apoE-/- male mice on high-fat diet （0.5 % cholesterol, 21% fat） were randomly divided into two groups and received i.p. injection of saline （100 μL/day） and DHI （100 μL/day）, respectively, for 16 weeks. At the end of experiment, we determined the development of atherosclerosis in en face aorta and aneurysms,pathological morphology of arterial wall, and serum lipid levels. We also determined the expression of monocyte chemoattractant protein-1 （MCP-1）, MMP-2, and MMP-9 mRNA in aortic wall using real-time RT-PCR. Our results indicated that high-fat diet induced the development of AAAs in apoE-/- mice, but the induction was totally blocked by DHI （P 〈 0.01）. The result of staining of abdominal aortic cross sections showed that DHI main- tained the collagen content in arterial wall, thereby pre- venting the animals from the development of AAA. Although DHI had little effect on serum total- and LDL- cholesterol levels, it reduced the expression of MCP-1, MMP-2, and MMP-9 mRNA in aortic wall （P 〈 0.01）. Taken together, our study suggests that DHI can inhibit the high-fat diet-induced AAA formation. The inhibitory effects may be related to the maintenance of the collagen content and inhibition of expression of AAA-related genes. Our study may suggest a new application of DHI in clinics.     

Antiinflammatory and analgesic effect of herbal cocktail Hongbaekjeong via inhibition of proinflammatory cytokines and prostaglandin E2 release

Though Hongbaekjeong(HBJ),a hebal mixture of three medicinal plants,has been traditionally used for arthritis and muscular pain,its scientific evidence still remains unclear.Thus,in the present study,analgesic and anti-inflammatory mechanism of HBJ was evaluated in vitro and in vivo.HBJ significantly reduced NO production and prostaglandin E2(PGE2)release and also attenuated the expression of cyclooxygenase 2(COX-2)in lipopolysaccharides(LPS)and interferon(IFN)-c treated RAW 264.7 cells.Furthermore,HBJ abrogated the production of proinflammatory cytokines such as interleukin(IL)1b,IL-6,IL-8 and monocyte chemoattractant protein-1(MCP-1)in LPS and IFN-c treated RAW 264.7 cells.In addition,HBJ significantly decreased the number of writhing syndrome induced by acetic acid,and also increased latency in hot-plate method and tail flick test in mice.Consistently,HBJ significantly reduced the edema volume in the hind paw of the rats with arthritis induced by Freund’s complete adjuvant(FCA)compared to untreated control.Collectively,our findings demonstrate the antiinflammatory and analgesic potential of HBJ via inhibition of proinflammatory cytokines and PGE2 release for treatment of arthritis and muscular pain.