药物致聋及肾损害的研究

对某聋哑学校的313名学生进行调查,发现由药物引起聋哑者153人,占50％,第一次尿检查采用尿潜血试纸及尿四联试纸同时进行,结果阳性蛋白尿、血尿、白细胞尿及菌尿共11例;第二次尿检查采用尿液分析仪及光学显微镜进行,结果阳性蛋白尿、血尿3例;第三次进行肾功能检查,结果均正常。药物致聋及肾损害经过8～16年后作远期观察,表明其对肾的影响并不大,但随着抗生素应用日增,药物致聋及肾损害的研究更应加以重视。     

CdTe量子点对人乳腺癌细胞MCF 7/ADM的化学增敏作用及其机制研究

利用纳米材料CdTe量子点作为增敏剂, 四甲基偶氮唑蓝(MTT)法检测细胞活力的实验表明CdTe量子点增强了阿霉素对于MCF 7/ADM的毒性, 而对非耐药的MCF 7无增敏作用. 然后利用免疫印迹法和免疫荧光染色法检测发现CdTe量子点提高了MCF 7/ADM细胞的自噬水平, 且这种自噬水平的上调与化学增敏效应是相关的, 以上结果提示CdTe量子点通过诱导MCF 7/ADM细胞发生自噬, 增强耐药性细胞对DOX毒性的敏感性.     

基于近红外光的人工流产手术系统设计

提出一种基于近红外光的人工流产手术系统的设计方案.人工流产吸引管采用CMOS图像传感器和近红外LED光源技术.在有血液的环境下,进行可见光与近红外光成像效果对比实验,并在此基础上,进行了不同波长近红外单色光成像效果对比实验,以确定CMOS摄像头在血液环境下的最佳红外波长.吸引管有3个腔道构成,分别为主吸引腔道、走线腔道、减压通道.人工流产吸引管的减压通道,在术中可以利用压差将宫腔内升高的压力以流体力学的方式排出,减少宫血逆流等并发症、促进血窦闭合、减少出血、减轻疼痛.     

Development of a nano-immunomodulator encapsulating R837 and caffeine for combined radio-/immunotherapy against orthotopic breast cancer

Immunotherapy is an ascendant approach in cancer treatment. It shows more pronounced effects on killing cancer cells in a specific manner in particular against metastasis more than traditional techniques, such as chemotherapy or surgery. However, tumor immunosuppression limits the response of the immune system to cancer development. In this study, we developed a lipid-based nanocarrier doubly loaded with imiquimod (R837), a toll-like receptor 7 agonist, and caffeine, an adenosine receptor antagonist. This R837/caffeine loaded nanocarrier served as a nano-immunomodulator (RC-nIM) for combination treatment with radiotherapy (RT) against orthotopic breast cancer. RT-induced immunogenic cell death facilitated the production of tumor antigen and elicited the immune response in corporation with R837-medaited activation of antigen-presenting cells (APCs) while RC-nIMs being adopted. Additionally, caffeine, an adenosine analog, can successfully compete with adenosine in the tumor. The tumor-bearing mice that received RT together with RC-nIMs experienced the best antitumor effects and exhibited higher levels of T cells and APCs within the tumor; the growth of secondary tumors was also limited. This work serves as a proof-of-concept study for the development of a new immunotherapy strategy against cancer.     

非嵌插类药物与DNA作用的分子机制

本文概述了非嵌插类药物与DNA作用的分子机制,考察了DNA螺旋小沟结合药物的碱基序列特异性,药物-DNA相互作用的性质和相互作用能,以及研究药物与DNA作用分子机制的意义。