果腹果蝇黑条体性状不稳定的遗传分析

将黑腹果蝇黑条体品系和黑条体新组合品系与遗传学实验常用的几个果蝇品系杂交，发现黑条体性状在Ｆ１和Ｆ２很不稳定，黑条件性状不稳定，可以与黑条体基因自发转座以及转座基因与多基因有关。     

果蝇NOMPC相互作用蛋白的筛选与鉴定

NOMPC是果蝇感受触觉的机械力敏感离子通道,属于瞬时受体电位(TRP)离子通道家族,机械力敏感离子通道通过怎样的机制被机械力激活?与NOMPC相互作用的蛋白有哪些?为了研究这些问题,本研究从果蝇NOMPC全长cDNA中扩增得到其N端的29个锚蛋白重复序列(ARs),ORF长度为3 039bp.然后将这29个ARs构建到表达载体pUAST-EGFP上,转染果蝇S2细胞后,通过免疫沉淀、质谱分析和Western blot筛选和鉴定NOMPC相互作用的蛋白,实验筛选出NOMPC N端的29ARs和NOMPC全长的相互作用蛋白,通过免疫共沉淀(Co-IP)实验,对可能与NOMPC存在相互作用的蛋白质进行验证,鉴定出Annexin B9、CG3195、CG3731、CG5374与NOMPC存在相互作用,为进一步研究NOMPC介导触觉转导的分子机制提供了基础.     

果蝇细胞周期蛋白Cyclin D在胚胎发育不同时期的表达

细胞周期蛋白Cyclin D（CycD）是发育中重要的蛋白,作者利用原位杂交法和免疫组化法,分别对野生型的果蝇w1118以及本研究室构建的一个转基因株系cycD-lacZ进行分析,从而建立了在果蝇发育的早期胚胎发育各阶段cycDmRNA的表达模式.结果表明：Stage 5时期,cycDmRNA集中于胚胎表面新生成的细胞处.Stage10,在发育中的神经系统,前中肠和后中肠处可以检测到cycD的mRNA.Stage14,cycD基因于中肠有着强烈的表达.cycDmRNA在胚胎发育中的表达模式的确立,为后期研究CycD在胚胎发育中所起的作用奠定了基础.     

黑腹果蝇杂合体中ADH杂二聚体的形成

研究了醇脱氢酶基因 (Adh)诱变体与正常基因相互作用后的部分显性现象 .所有 8个由乙基亚硝基尿素 (ENU)和 1个 X-射线诱变体仅为单碱基置换体 ,其余 3个 X-射线诱变体则为 9～2 1个碱基的缺失体 .这 1 2个诱变体 (除 1个外 )都能产生可测的突变肽 ,其中 7个不能与正常肽形成二聚体 ,杂合体酶活性约为正常纯合体的 1 /2 ;另 4个形成二聚体 .形成二聚体突变基因产物中所有氨基酸突变均发生在肽链 1 82～ 1 94氨基酸区域 ,可见该区域对于二聚化不是必需的 ,该序列可能是重要的催化表面功能区     

Climatic adaptations and distributions in the Drosophila takahashii species subgroup (Diptera: Drosophilidae)

Among subtropical species of the Drosophila takahashii species subgroup, temperature adaptations were related to altitudinal distribution; D. trilutea was adapted to lower temperature and occurred at high altitudes in September in Taiwan, D. takahashii was adapted to higher temperature and occurred mainly at low altitudes, and D. prostipennis was adapted to an intermediate range of temperature and occurred at mid to high altitudes. A temperate species, D. lutescens, was adapted to a wider range of temperature than the subtropical species: it was more cold-hardy than all three subtropical species and more heat-tolerant than D. prostipennis and D. trilutea. The temperature ranges encountered in the temperate region far exceed the range in subtropical areas, and this is likely to be reflected in the temperature adaptations of these temperate and subtropical species. D. lutescens has a photoperiodically controlled reproductive diapause. No latitudinal cline was observed on cold-hardiness of D. takahashii. By contrast, cold-hardiness and photoperiodic response differed between northern and southern populations of D. lutescens.     

果蝇精子发生中CG15899的基因表达

为了检测在雄性果蝇中CG15899基因是否有特殊的表达,利用PCR的方法得到CG15899的基因片段,并用DIG标记PCR产物,将其与野生型雄性果蝇melanogaster的精巢进行原位杂交,最后在果蝇精巢的细胞质中产生了较强的信号．实验结果表明,CG15899基因在精原细胞和初级精母细胞中表达．     

人RP2基因在果蝇胚胎细胞中的表达

将质粒pJLA503中的RP2编码cDNA用PCR的方法扩增,并与pIND-3myc质粒上的人c-myc编码序列连接到果蝇转基因载体pUAST上,构建了UAS－RP2－3myc和UAS－RP2(del6)-3myc融合质粒。用脂质体转染的方法,将质粒导入果蝇培养细胞系Schneider line2(S2),并用PCR加以证实,然后通过Western blot检测证明了RP2和RP2 del6的表达。证明了人的RP2基因在果蝇细胞中表达的可能性,说明果蝇系统是可以用来分析人的RP2基因功能的。     

Sensory projections from dorsal and ventral appendages inDrosophila grafted to the same site are different

Summary Owing to a new transplantation technique, we have been able to study the sensory projections of homologous and heterologous appendages grafted to the same abdominal site inD. melanogaster. Axons from homologous transplants exhibit similar terminal patterns, whereas those from heterologous transplants do not. It is suggested that ectopic sensory axons specifically recognized central areas and pathways occupied by axons from homologous appendages.     

Acetaldehyde oxidation inDrosophila null-mutants for alcohol dehydrogenase

Summary Aldehyde dehydrogenase (ALDH) activity is demonstrated in four strains ofD. melanogaster lacking active alcohol dehydrogenase (ADH-null mutants). In the four strains, ALDH activities are similar to those found in a wild strain. It is concluded that ADH-null flies are able to detoxify acetaldehyde. This finding is discussed in relation with the dual function of ADH proposed recently.This work was supported by a grant from the Medical Research Council of Canada (MRC 6920) to Dr F. Garcin.