反相高效液相色谱法测定龙血竭中剑叶龙血素C的含量

建立反相高效液相色谱法测定龙血竭中剑叶龙血素C含量的方法，以Phenomenex C18反相键合硅胶色谱柱为固定相，水-乙腈(33：67)为流动相，流速1ml／min，检测波长为211nm；柱温35℃；用外标法定量。结果表明，剑叶龙血素C在42．85～342．8μg进样范围内有良好线性关系(r=0．9996)，方法回收率为97．87％。本方法简便、准确，重复性好，可作为龙血竭中剑叶龙血素C的含量测定方法。     

叔丁氯喘通改善猪肉品质的研究

用β_2——肾上腺素受体兴奋剂——叔丁氯喘通处理去势汉白杂交猪,并对其肥育性能、肉质以及肉和血中诸项生化指标进行了分析研究。结果表明,叔丁氯喘通提高瘦肉率10.69％,眼肌面积21.97％,后腿比28.69％(P<0.05),降低了背膘厚,肉中脂肪含量降低12.30％,蛋白质含量提高8.60％。处理后,血糖、血脂、脂肪酶活性增加和血中非蛋白氮含量降低。     

雪莲对兔“热毒血瘀证”模型血液凝固性及血小板免疫粘附功能的影响

检测血液凝固性和血小板免疫粘附功能是研究血瘀证实质和活血化瘀药理作用的重要手段。为了探讨雪莲活血化瘀的药效及作用机制,本实验对沙门氏菌性“热毒血瘀”模型兔给药前后的血小板数、血小板电泳速率、血小板聚集性、体外血栓形成、血浆纤维蛋白原含量、优球蛋白溶解时间及血小板免疫粘附功能进行了检测。证明雪莲对二磷酸腺苷(ADP)诱导的正常兔和“热毒血瘀”模型元的血小板聚集性具有很强的抑制作用,其抑制率分别为21.1±2.9％(±Sx)和25.6±2.7％(±Sx),且对模型兔血小板聚集性的抑制作用要比对正常兔的强(P<0.01);雪莲对亢进的血小板免疫粘附功能具有抑制作用。此外,雪莲还具有抗体外血栓形成及抗机体纤溶活性降低的作用。但雪莲对血小板的表面电荷无影响,对血小板的生成也无明显促进作用。结果表明,雪莲的活血化瘀作用机制与其促进机体纤溶活性和降低血液凝固性有着密切关系。     

Biomedicine and diseases: the Klippel-Trenaunay syndrome, vascular anomalies and vascular morphogenesis

Vascular morphogenesis is a vital process for embryonic development, normal physiologic conditions (e.g. wound healing) and pathological processes (e.g. atherosclerosis, cancer). Genetic studies of vascular anomalies have led to identification of critical genes involved in vascular morphogenesis. A susceptibility gene, VG5Q (formally named AGGF1), was cloned for Klippel-Trenaunay syndrome (KTS). AGGF1 encodes a potent angiogenic factor, and KTS-associated mutations enhance angiogenic activity of AGGF1, defining ‘increased angiogenesis’ as one molecular mechanism for the pathogenesis of KTS. Similar studies have identified other genes involved in vascular anomalies as important genes for vascular morphogenesis, including TIE2, VEGFR-3, RASA1, KRIT1, MGC4607, PDCD10, glomulin, FOXC2, NEMO, SOX18, ENG, ACVRLK1, MADH4, NDP, TIMP3, Notch3, COL3A1 and PTEN. Future studies of vascular anomaly genes will provide insights into the molecular mechanisms for vascular morphogenesis, and may lead to the development of therapeutic strategies for treating these and other angiogenesis-related diseases, including coronary artery disease and cancer.Received 24 November 2004; received after revision 21 January 2005; accepted 2 March 2005     

玛咖与西洋参缓解体力疲劳作用对比试验研究

目的初步研究玛咖和西洋参对缓解体力疲劳的作用。方法用四批动物,每批70只昆明种小鼠,按体重分成7组,分别给予去离子水,0.040、.4和1.2g/kg玛咖;0.04、0.4和1.2g/kg西洋参,连续灌胃30d后,分别测定四批小鼠负重游泳时间及运动后即刻的血清尿素、肝糖原的含量;运动前、运动后即刻及运动后休息20min的血乳酸含量。结果0.4g/kg和1.2g/kg玛咖可明显延长小鼠负重游泳时间(与阴性组比较P<0.05和P<0.01),0.04和0.4g/kg西洋参能明显延长小鼠负重游泳时间(与阴性组比较P<0.01和P<0.05);3剂量组的玛咖对小鼠血清尿素无明显影响(与阴性组比较P>0.05),1.2g/kg西洋参可显著降低小鼠运动后的血清尿素水平(与阴性组比较P<0.05);3个剂量的玛咖与西洋参均可增加小鼠的肝糖原含量;0.4和1.2g/kg玛咖可降低运动前后血乳酸变化值(与阴性对照组比较P<0.01),各剂量的西洋参对小鼠运动前后血乳酸变化值无明显影响。结论玛咖和西洋参都具有缓解体力疲劳的功能,但两者对小鼠血乳酸和尿素氮的影响不同。     

实验性大鼠血脂检测

目的　检测普通级和清洁级SD大鼠血清总胆固醇 (TC)和甘油三酯 (TG)值。方法　TC测定采用酶法 (终点法 CHO PAPMethod) ,TG采用酶法 (终点法 GPO PAPMethod)。结果　 (1)普通级雌性SD大鼠血清TC和TG值分别为 (1 67± 0 2 4)mmol L和 (0 52± 0 12 )mmol L ;(2 )普通级雄性SD大鼠血清TC和TG值分别为 (1 52± 0 18)mmol L和 (0 58± 0 14 )mmol L ;(3 )清洁级雌性SD大鼠血清TC和TG值分别为 (1 80± 0 3 2 )mmol L和 (0 43± 0 0 9)mmol L ;(4)清洁级雄性SD大鼠血清TC和TG值分别为 (1 60± 0 42 )mmol L和 (0 75± 0 3 0 )mmol L。结论　本实验为洁净实验动物正常血液测定参考系统的确立提供一定的参考数据     

Chinese medicine, flowing blood, biomechanopharmacology and future preventive medicine

Activating Blood Circulation to Remove Blood Stasis(ABCRBS) has long been the most fundamental therapeutic principle since the age of Yellow Emperor(circa 2600 BC) in Chinese medicine.Based on bio-responses of blood shear stress,which was not considered in Chinese medicine,we have initiated the merging of biomechanics with pharmacology and Chinese medicine,establishing the new discipline-biomechanopharmacology.Practice of exercise is a simple and self-regulatory way to promote shear stress in the circulation.Interestingly,acupuncture and massage are also linked with shear stress regulation.These procedures are expected to maintain normal functions of endothelial cells(ECs) by regulating shear stress levels of flowing blood.The statement of "Diseases are prevented as blood flow is promoted" by the renowned ancient physician Huatuo,who presented the wise foresight of the efficacy of shear stress,would likely begin a new chapter in preventive pharmacology and medicine in the years to come.     

内蒙古民族大学152名教师血脂检测结果分析

目的:了解教师血脂水平,通过健康教育改变不良生活方式,预防心脑血管疾病.方法:对152例教职工进行血脂检测;胆固醇和甘油三酯分别采用酶法测定.结果:高血脂检出率较高,60岁以前血TC(总胆固醇)和TG(甘油三酯)异常检出率随着年龄的增高而增高,且50岁前男性异常检出率高于女性,50岁以上的女性TC和TG异常率高于同年龄组的男性.结论:预防和控制高血脂,对不同性别进行针对性的健康指导和有效干预,合理饮食、加强体育锻炼,减少心血管疾病的发生.     

脉搏波特征点的自动识别方法

随着无创血液循环动力学监测技术和微机技术的发展,研究脉博波特征点的自动识别方法是很有意义的.波形识别方法目前主要有阈值法、轮廓限制法、面积法、数字滤波法、句法方法和斜率法等,这些方法已应用于心电图的自动识别.根据脉搏波的特     

多烯康对糖尿病合并脑梗塞血液流变的影响

糖尿病合并脑梗塞是糖尿病患者常见的脑血管并发症 ,经多烯康治疗 ,治疗前后其血液流变学的各项指标差异显著 (P <0 0 1) ,提示多烯康是治疗糖尿病合并脑梗塞的有效良药 .