基于DNA分子的逻辑门与计算机

本文对基于DNA分子的逻辑门与计算机研究进行了综述。DNA逻辑门与DNA计算机是目前非常活跃的研究领域,有可能解决电子计算机发展的瓶颈问题。本文从DNA逻辑门的分子基础、DNA逻辑门和DNA计算机等几个方面进行了介绍,并且对可能的发展方向作了一些预期。     

Evaluation of the introgressed lines and screening for elite germplasm in Gossypium

THERE ARE 51 SPECIES IN THE GENUS OF GOSSYPIUM[1]. EXCEPT UPLAND COTTON CULTIVARS, WILD CULTIVARS, SPECIES AND RACE HAVE ABUNDANT GENETIC POLYMORPHISMS DUE TO THEIR VARIOUS ENVIRONMENTAL DISTRIBUTION AND LONG-TERM NATURAL SELECTION. THEREFORE, THEY POSSESS LOTS OF EXCEL- LENT GENES THAT CAN BE USED TO EXPLOIT POTENTIAL TRAITS, SUCH AS DROUGHT RESISTANCE, DISEASE AND INSECT …     

Comparative analysis of A, B, C and D genomes in the genus Oryza with Cot-1 DNA of C genome

Fluorescence in situ hybridization （FISH） was applied to somatic chromosomes preparations of Oryza officinalis Wall. （CC）, O. sativa L. （AA）xO. officinalis F1 hybrid （AC）, backcross progenies BC1 （AAC and ACC）, O. latifolia Desv. （CCDD）, O. alta Swallen （CCDD） and O. punctata Kotschy （BBCC） with a labelled probe of Cot-1 DNA from O. officinalis. In O. officinalis, the homologous chromosomes showed similar signal bands probed by Cot-1 DNA and karyotype analysis was conducted based on the band patterns. Using no blocking DNA, the probe identified the chromosomes of C genome clearly, but detected few signals on chromosomes of A genome in the F1 hybrid and two backcross progenies of BC1. It is obvious that the highly and moderately repetitive DNA sequences were considerably different between C and A genomes. The chromosomes of C genome were also discriminated from the chromosomes of Dand B-genome in the tetraploid species O. latifolia, O. alta and O. punctata by Cot-1 DNA-FISH. Comparison of the fluorescence intensity on the chromosomes of B, C and D genomes in O. latifolia, O. alta, and O. punctata indicated that the differentiations between C and D genomes are less than that between C and B genomes. The relationship between C and D genomes in O. alta is closer than that of C and D genomes in O. latifolia. This would be one of the causes for the fact that both the genomes are of the same karyotype （CCDD） but belong to different species. The above results showed that the Cot-1 DNA had a high specificity of genome and species. In this paper, the origin of allotetraploid in genus Oryza is also discussed.     

拟黑多刺蚁(Polyrhachis vicina)性腺雌激素相关受体基因的表达

采用原位杂交方法对拟黑多刺蚁(Polyrhachis vicina)蚁后卵巢中和雄蚁精巢中pvERR mRNA的表达进行了检测.结果表明,pvERR mRNA在卵子发生过程中滤泡细胞的胞质和生长期、卵黄发生期的卵母细胞有表达.在变形期的精细胞胞质和成熟精子头部有很强的表达.     

适用于空间环境的核酸杂交芯片技术研究

核酸杂交芯片是一种目前已经被广泛应用的核酸分析技术手段.在空间微重力条件下,利用小型化低功耗的检测设备,实现核酸杂交芯片功能,获得准确可信的核酸杂交检测结果,是一项非常具有挑战性的课题.本文对核酸杂交芯片在空间条件下的应用方法进行了探索,采用新型核酸杂交芯片结构、改变核酸杂交芯片反应条件、串联核酸PCR芯片系统等手段,开发出一套小型化低功耗的空间核酸杂交分析芯片,芯片系统整体功耗小于25 W,实现了对目标核酸序列的快速检测(检测周期90 min),并初步实现与空间聚合酶链式反应(PCR)等技术的联合应用,提高了核酸杂交芯片检测技术的检测灵敏度.     

纳米半球壳结构光学调谐特性分析

利用离散偶极近似法,理论研究了颗粒几何结构参数和物理特性对金纳米半球壳颗粒消光特性的影响.结果表明,随着壳层厚度的增大,消光共振波长先发生蓝移而后再红移,共振波长转向时对应的壳层厚度随内核材料及外界环境介电常数和颗粒间距的增大而增大.利用等离激元杂化理论和相位延迟效应对该现象进行了解释.     

Molecular cloning of rice cDNAs related to pollen development using the subtraction hybridization technique

The meiotic stage of pollen mother cell is a very important stage in controlling the development and formation of pollen. In order to clone the rice cDNA(s) of this stage, a normal rice, Annong N and its thermosensitive mutant, Annong S-1 were used as the plant material. The mRNA has been extracted from the young panicle at the meiotic stage. By using the cDNA subtraction hybridization technique, three cDNA fragments, RP-1, RP-2 and RP-3 have been successfully cloned from Annong N. Northern blot analysis reveals that the mRNA of these three clones are expressed only in anthers, and not leaves. The mRNA levels of these clones are lower in anthers of Annong S-1 than in Annong N. Furthermore, the amount of mRNA extracted from anthers of Annong S-1 growing under high temperature (28℃) is lower than plants growing at lower temperature (25℃). Sequence analysis and homology search indicate that these three clones display no similarity to the current database. It is concluded that the three novel cDNA cloned are related to pollen development in rice.     

单只不育果蝇未知基因的反向PCR

根据ｐ转位子诱变和ｐｏｘｎ基因双杂合子法检测化学感受器数目的变化,筛选到一系列突变个体,其中一个雄蝇腿部表现出不能形成化学感受器的突变表型;遗憾的是该只雄蝇是不良的,为了克隆该基因,研究出一种微量ＤＮＡ反向ＰＣＲ法,该法可用０．５只果蝇扩增出Ｐ转位子插入点相邻的基因组片断,方法简便可靠。