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91.
高压静电场对鲱鱼DNA X射线衍射谱的影响 总被引:1,自引:0,他引:1
用高压静电场处理鲱鱼DNA,测试电场处理前后鲱鱼DNA的X射线衍射谱,衍射谱的变化表明高压静电场可改变DNA的构象。 相似文献
92.
93.
水溶性高分子金属卟啉络合物的合成及其与DNA作用的研究 总被引:2,自引:0,他引:2
利用荧光探针技术和圆二色谱等分析手段对两种水溶性高分子金属卟啉络合物同DNA的作用进行了初步研究.实验表明,所合成的高分子金属卟啉络合物在同DNA作用时存在有嵌入模式,金属卟啉络合物大的平面环疏水结构和亲水性基团的引入促进了这种作用. 相似文献
94.
Lee Imshik Li Qing Yang Linjing Wang Xinwen Deng Wenli Wang Chen Bai Chunli 《科学通报(英文版)》1997,42(18):1581-1581
Conclusion A py-pu-py triplex DNA containing the polarity modification by replacing the atom H5 of cytosine with Br was examined, mainly
for the stability of the formation of the triplex DNA. Our computational results show that the conformation of the strand
that contained a replaced H5 by cytosine to Br was destabilized, but the Hoogsteen base pairs were stabilized. Although this
approach of modifying the polarity of DNA base has not been tried before, it shows the possibility to improve the stability
by the polarity modification. 相似文献
95.
Kangdi Hu Wanjie Li Jiaxin Gao Qizheng Liu Haitao Wang Yue Wang Jianli Sang 《科学通报(英文版)》2014,59(31):4060-4068
To study the function of CaPptl, we deleted PPT1 gene from the Candida albicans genome by sequentially replacing the entire coding region with the selectable markers ARG4 and HIS1. The results showed that the deletion of Pptl did not affect the hyphal formation of C. albicans under serum induction and caused enhanced sensitivity to DNA damage, Calcofluor white and salt- induced stress. We also found that Pptl was not required for the phenotypic response of cells treated with the genotoxins, methylmethane sulfonate and hydroxyurea. Flow cytometric analyses indicated that pptlA cells and wild-type cells showed similar G2/M arrest profiles when exposed to DNA damage stress. Pptl was not required for the activation of the DNA damage response pathway, as indicated by normal phosphorylation of Rad53 and Rfa2 in pptlA cells under DNA damage stress. We suggest that Pptl plays important roles in response to various stress conditions in C. albicans. 相似文献
96.
一种适合芽孢杆菌质粒DNA提取的改良碱裂解法 总被引:6,自引:0,他引:6
以Birnboim和Doly的质粒提取方法为基础,通过缩短菌体培养时间,增加菌体收集量,并对提取过程中所用的三种主要溶液的用量进行适当调整等,摸索出质粒提取的一种改良碱法.用改良后的碱法对浸麻芽孢杆菌、地衣芽孢杆菌和枯草芽孢杆菌进行质粒提取,均获成功. 相似文献
97.
A novel DNA coding based knowledge discovery algorithm was proposed, an example which verified its validity was given. It is proved that this algorithm can discover new simplified rules from the original rule set efficiently. 相似文献
98.
Minagawa H Yoshida Y Kenmochi N Furuichi M Shimada J Kaneko H 《Cellular and molecular life sciences : CMLS》2007,64(1):77-81
Lactate oxidase is used in biosensors to measure the concentration of lactate in the blood and other body fluids. Increasing
the thermostability of lactate oxidase can significantly prolong the lifetime of these biosensors. We have previously obtained
a variant of lactate oxidase from Aerococcus viridans with two mutations (E160G/V198I) that is significantly more thermostable than the wild-type enzyme. Here we have attempted
to further improve the thermostability of E160G/V198I lactate oxidase using directed evolution. We made a mutant lactate oxidase
gene library by applying error-prone PCR and DNA shuffling, and screened for thermostable mutant lactate oxidase using a plate-based
assay. After three rounds of screening we obtained a thermostable mutant lactate oxidase, which has six mutations (E160G/V198I/G36S/T103S/A232S/F277Y).
The half-life of this lactate oxidase at 70 °C was about 2 times that of E160G/V198I and about 36 times that of the wild-type
enzyme. The amino acid mutation process suggests that the combined neutral mutations are important in protein evolution.
Received 15 September 2006; received after revision 21 October 2006; accepted 2 November 2006 相似文献
99.
100.
最短路问题的闭环DNA算法 总被引:1,自引:0,他引:1
提出了不等长闭环DNA分子的概念,由此推广了闭环DNA计算模型。给出了固定端点的最短路问题闭环DNA算法,该算法首先对每条弧进行了三组DNA编码,再用有目的的终止技术合成固定端点的所有链,然后通过接入实验和电泳实验得到最短路,并通过检测实验输出所有最短路径。得出了算法的复杂性,为说明算法的有效性给出了一个算例。最后讨论了最短路问题闭环DNA算法在变权网络、自由终点或固定中间点的最短路问题中的应用,并给出了相应的解决方法。由此说明该算法具有广泛的适应性。 相似文献