高压静电场对鲱鱼DNA X射线衍射谱的影响

用高压静电场处理鲱鱼DNA,测试电场处理前后鲱鱼DNA的X射线衍射谱,衍射谱的变化表明高压静电场可改变DNA的构象。     

水溶性高分子金属卟啉络合物的合成及其与DNA作用的研究

利用荧光探针技术和圆二色谱等分析手段对两种水溶性高分子金属卟啉络合物同DNA的作用进行了初步研究．实验表明,所合成的高分子金属卟啉络合物在同DNA作用时存在有嵌入模式,金属卟啉络合物大的平面环疏水结构和亲水性基团的引入促进了这种作用．     

Polarity modified triplex DNA by bromide

Conclusion A py-pu-py triplex DNA containing the polarity modification by replacing the atom H5 of cytosine with Br was examined, mainly for the stability of the formation of the triplex DNA. Our computational results show that the conformation of the strand that contained a replaced H5 by cytosine to Br was destabilized, but the Hoogsteen base pairs were stabilized. Although this approach of modifying the polarity of DNA base has not been tried before, it shows the possibility to improve the stability by the polarity modification.     

基于矩形宏模块的片上系统布图规划算法

布图规划和布局是集成电路片上系统物理设计的基本问题之一。总结了作者提出的几个新的布图规划和布局优化算法及其特点。基于随机优化算法 ,提出了角模块序列表示方法和基于这一表示方法的包含各种布图约束的布图规划和布局算法。提出的基于约束图的布图规划表示 ,为研究序列队 (SP)、变形网格 (BSG)、有序树 (O- tree)等表示之间的关系奠定了理论基础。基于“最小自由度优先原则”,提出了迄今最快的确定性布图规划与布局算法。提出了基于解空间平滑的布图规划和布局算法。该算法与基于模拟退火的算法相比性能更加健壮稳定。在解空间平滑中首次考虑了局部平滑效应 ,这是对解空间平滑方法从原理上的重要改进     

一种适合芽孢杆菌质粒DNA提取的改良碱裂解法

以Birnboim和Doly的质粒提取方法为基础,通过缩短菌体培养时间,增加菌体收集量,并对提取过程中所用的三种主要溶液的用量进行适当调整等,摸索出质粒提取的一种改良碱法.用改良后的碱法对浸麻芽孢杆菌、地衣芽孢杆菌和枯草芽孢杆菌进行质粒提取,均获成功.     

DNA Coding Based Knowledge Discovery Algorithm

A novel DNA coding based knowledge discovery algorithm was proposed, an example which verified its validity was given. It is proved that this algorithm can discover new simplified rules from the original rule set efficiently.     

Improving the thermal stability of lactate oxidase by directed evolution

Lactate oxidase is used in biosensors to measure the concentration of lactate in the blood and other body fluids. Increasing the thermostability of lactate oxidase can significantly prolong the lifetime of these biosensors. We have previously obtained a variant of lactate oxidase from Aerococcus viridans with two mutations (E160G/V198I) that is significantly more thermostable than the wild-type enzyme. Here we have attempted to further improve the thermostability of E160G/V198I lactate oxidase using directed evolution. We made a mutant lactate oxidase gene library by applying error-prone PCR and DNA shuffling, and screened for thermostable mutant lactate oxidase using a plate-based assay. After three rounds of screening we obtained a thermostable mutant lactate oxidase, which has six mutations (E160G/V198I/G36S/T103S/A232S/F277Y). The half-life of this lactate oxidase at 70 °C was about 2 times that of E160G/V198I and about 36 times that of the wild-type enzyme. The amino acid mutation process suggests that the combined neutral mutations are important in protein evolution. Received 15 September 2006; received after revision 21 October 2006; accepted 2 November 2006     

一种基于标准单元芯片电源线网的静态电压降的分析方法

提出了一种对基于标准单元芯片计算其电源线网的静态电压降的分析方法.该方法通过计算每个标准单元的功耗得到其电流源模型,然后采用导线电阻模型和标准单元等效电流源模型生成电路网络,运用逐次块松弛迭代方法求解由上述网络生成的改进节点法方程.仿真结果表明,所提出的方法能与SPICE达到同样的精度,且计算速度更快,使用内存更少.     

PCI总线接口卡的设计与实现

介绍了基于CH365芯片的PCI总线接口卡的设计与制作,利用该技术可以对传统ISA总线接口卡进行低成本技术改造,以及设计工业现场控制PCI接口卡,具有较强的实用性和较高的市场推广价值。