Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity

The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB.     

导入野生大豆DNA小麦后代的农艺性状研究

用花粉管通道法将野生大豆总DNA导入小麦，获得了转基因小麦，其后代的农艺性状有较大的变化。田间实验分析了变异系小麦植株与对照植株在叶片的光合速率、蒸腾速率、叶面积及株高、穗粒、穗重等性状上的差异。t检验结果显示，变异系小麦在叶面性状及产量性状上的差异是显著的，外源DNA的导入改善了植物的农艺性状，并在后代中表达，为选育高产、优质的新小麦品种提供了依据。     

智者的尴尬——简析中西文化差异

地域、审美、民族性格的不同，导致中西文化的巨大差异，从对“智慧”的不同审美倾向，揭示出两种文化的内在动因和文化的遗传。     

6种瓢虫的RAPD分析及在分类上应用的研究

利用随机引物分别对6种瓢虫的DNA进行了聚合酶链式反应（RAPD－PCR）的实验。结果显示，用相同引物扩增不同种瓢虫的基因组DNA，扩增产物多态性DNA片段的数目是不同的；两种不同引物的扩增产物中，各自呈现出种、属的特异性片段，其可用于种、属的鉴别；聚类图显示了种、属之间亲缘关系的远近程度，并与形态学分类结果相一致。实验分析还发现：七星瓢虫不同个体间多态性DNA特有带较多，遗传较稳定；龟纹瓢虫与之相比，不同个体间多态性DNA的特有带较少，变异程度较大。     

Optimum Combined Lenses for Confocal Biochip Scanning System

IntroductionLaboratory-on-a-chip technology[1 5] has gainedinterest in recent years,and consists of threeclassic steps:sample preparation,biochemicalreaction,and detection analysis.Many researchgroups have used small biochips to studypolymerase chain reaction (PCR) amplification[1 ] ,DNA sequencing[2 ] ,ligase chain reaction(LCR) [3] ,dielectrophoresis of cell separation,andcapillary electrophoresis[4 ,5] .Some importantbiochip detection systems[6 8] have been developed,such as the dual-la…     

四种过渡金属对DNA稳定性影响的研究

本文用紫外光谱法研究了 Fe~(3+)、Co~(2+)、Ni~(2+)、Zn~(2+)四种过渡金属对 DNA 稳定性影响及与 DNA配位方式,结果表明 Fe~(3+)、Co~(3+)有稳定 DNA 的作用,Zn~(2+)、Ni~(2+)使 DNA 稳定性降低.     

大豆自花授粉后外源DNA导入技术的验证

以花生DNA为供体,大豆为受体,在大豆自花授粉后,采用液滴法和注射法导入花生DNA,在受体大豆植株后代中获得了多种变异类型。为探讨外源DNA 导入技术的可靠性,将具有卡那霉素抗性基因的质粒pCaMVneo 用同法导入大豆。所收获的种子发芽后培养于一定浓度的卡那霉素溶液中,其中部分植株对卡那霉素表现抗性,可继续生长发育。分株提取这些植株的DNA,以质粒DNA 为探针,进行斑点杂交.供试的14个植株中出现了13个阳性斑。实验结果证明质粒DNA 已整合到大豆基因组中并获表达,从而间接地证实了授粉后通过花粉管通道导入外源DNA 的技术是可靠的。     

黄瓜外源 DNA 导入技术方法研究

依据黄瓜的花器结构及开花习性,研究了通过花粉管通径将外源DNA 导入黄瓜子房的方式以及DNA 溶液的浓度、用量、pH 值和黄瓜花粉粒的渗透压等因紊对导入效果的影响.采用花粉与DNA 溶液混合授粉和子房微量注射均可成功的获得10~(-2)～10~(-3)的高遗传转化率.证明了应用外源DNA 导入进行黄瓜分子育种的可行性.     

小牛胸腺DNA的激光喇曼谱

用常规喇曼和表面增强喇曼的方法获得了小牛胸腺ＤＮＡ固体纤维和水溶液的激光喇曼谱，样品的喇曼特征谱带与国外文献及国内紫外共振喇曼法得到的谱图基本一致，由此可分析出样品的构象，基团归属等信息，证明喇曼光谱是用于测量生物分子结构的一个有力武器。