ICP-AES光谱仪的研制及其分析软件的设计

目前,市场上的ICP光谱仪仍以国外仪器为主,国内仪器的开发与研制始终未能有所突破,在仪器分析软件上仍属空白,这极大限制了国内ICP光谱仪的发展.本文主要针对单道顺序扫描型ICP-AES光谱仪的硬件及分析软件的开发提供了思路.     

核动力装置模拟器就地系统中双机通讯的实现

利用微机软仿真实现模拟器就地系统中仪表的动态实时显示和开关的实时控制，可以节省的大量外部设备，提高系统可扩充性。本文介绍了该技术实现的方法，即服务器与客户机之间数据双向通讯的实现途径。经实践验证，本文的方法是正确、可行的。     

Science Letters： A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen

It has been proposed that activation of Toll-like receptors （TLRs） plays crucial roles in the polarization of adaptive immune responses. A synthetic Toll-like receptor 2 （TLR2） ligand, Pam3CSK4, has been reported to modulate the balance of Th1/Th2 responses. We evaluated the modulation effect of Pam3CSK4 on allergic immune response in a mouse rhinitis model sensitized to house dust mite allergen （HDM）. Mice were sensitized and challenged with Dermatophagoidesfarinae allergen （Der f）, and then the allergic mice were treated by Pam3CSK4. Nasal allergic symptoms and eosinophils were scored. Der f-specific cytokine responses were examined in the splenocytes and bronchoalveolar lavage fluid （BALF）. Serum level of total IgE was also detected. After establishing a mouse allergic rhinitis model with HDM, we have showed that Pam3CSK4 treatment not only ameliorated the nasal allergic symptoms remarkably but also decreased the eosinophils and total inflammation cells in BALF significantly. Analysis of cytokine profile found that IFN-7 released from either BALF or stimulated splenocytes increased markedly in Pam3CSK4-treated mice, while IL-13 decreased significantly. Moreover, serum level of total IgE was significantly lower in Pam3CSK4-treated mice than in the untreated. Thus, in an allergic rhinitis mouse model developed with HDM, Pam3CSK4 was shown to exhibit an antiallergic effect, indicating its potential application in allergic diseases.     

Specific anti-tumor effect induced by attenuated Salmonella typhimurium vaccine expressing extracellular region of vascular endothelial growth factor receptor 2

The purposes of this research were to study the stable expression of exogenous gene encoding therapeutic protein in attenuated Salmonella typhimurium, observe the metabolism of oral gene vaccine carried by attenuated Salmonella typhimurium in BALB/c mouse, and investigate the feasibility of prevention and treatment of tumors by the recombinant bacteria. Recombinant plasmid pcDNA3.1 VEGFR2(n1-7) was transformed into competent attenuated Salmonella typhimurium SL3261 to develop oral DNA vaccine SL3261-pcDNA3.1 VEGFR2(n1-7). To observe whether the exogenous gene can be expressed in the recombinant bacteria, PCR was performed to amplify the CMV promoter of the eukaryotic expression vector as the proof of stable expression of exogenous protein; transmission elec- tron microscopy (TEM) was applied to observe the morphology of the recombinant bacteria to confirm that the exogenous gene has no impact on the growth of the bacteria, and then BALB/c mice were immunized with the gene vaccine. After inoculation of the gene vaccine, the recombinant bacteria SL3261 could be detected in the tissues such as small intestine, colon, liver and spleen. And then, mice in each group were challenged with tumor cells. The results of animal experiment showed that tumor growth of the mice in experimental group was inhibited and survival time of immunized mice was prolonged compared with control groups. A higher lymphocyte infiltration in tumors from animals treated with DNA vaccine was observed. Immunohistochemical analysis of tumor samples revealed an en- hanced accumulation of CD8 cytotoxic T lymphocytes, as well as an increase in CD4 cells in the tumors of animals treated with the oral gene vaccine compared to tumors from control group mice. Ultrastructure of the tumor tissue showed that tumor cells in the samples of the immunized mice were well-differentiated. Our research confirmed that the exogenous gene can be stably expressed in the attenuated Salmonella typhimurium and has no impact on the growth of the recombinant bacteria; the exogenous gene can de delivered to the host by attenuated Salmonella typhimurium to produce anti-tumor effect with no obvious cytotoxity to the host. In this study, it is established that attenuated Salmonella typhimurium could be used as a vector for oral gene vaccine, and our study provided a theoretical basis for the body distribution and the metabolism of the recombinant bacteria. This strategy may provide a simple, safe and effective way for the prevention and treatment of tumors.     

我国存货准则与IAS2的比较与思考

我国已经颁布了新的存货准则,并于今年年初开始实施,这对于进一步规范企业存货的确认、计量和披露,提高会计信息的相关性和可靠性,完善我国会计准则体系,推动我国证券市场乃至整个社会主义市场经济的发展,将起到积极的作用.     

Eph受体家族和Ephrin-A5在NSCLCs中上调表达及其临床意义

采用荧光实时定量PCR的方法在48个非小细胞肺癌组织以及配对的正常肺组织中检测Eph/ephrin 基因的mRNA水平.发现肺癌组织中EphB4, EphB3 和 ephrin-A5的表达水平与配对的正常肺组织相比分别上调了60%,81%和65%,并具有显著的统计学差异. 而且,统计学分析发现ephrin-A5的表达水平与肿瘤分型、转移、性别、吸烟以及诊断年龄等临床病理学参数之间存在显著的相关性.此外,EphA2的表达水平与肿瘤大小、家族遗传史以及诊断年龄之间也存在显著的相关性.总之,这些结果提示EphB4, EphA2, EphB3 和ephrin-A5 在肺癌的发生和发展过程中可能以一种单独或者协同的形式发挥作用.这些蛋白有可能成为研究和诊断肺癌的重要靶向基因.     

Effects of self evaluation on late ERP components

High-density event-related potentials (ERPs) were measured when participants were required to evaluate oneself, significant others (father and mother) and the most familiar public figures (Lei Feng and Jiang Qing) based on trait adjectives. There were three referential conditions and a non-referential control condition including self-evaluation (SE), significant other-evaluation (SOE), general other-evaluation (GOE), and semantic positivity-evaluation (SPE). The behavioral data showed that RTs to GOE condition were longer than the other three conditions. Scalp ERP analysis revealed that (1) SPE condition elicited a more negative N2 than SE condition, which was associated with inhibition of responses; (2) SE condition elicited a more positive P300 than SPE condition, which was an index of attention to self-relevant stimuli; (3) there was no difference on the amplitude and latency of P300 between SE and SOE conditions, which reflected that the concept of “self” included intimate others (e.g., father and mother) in Chinese culture.     

学生管理中的沟通策略——浅谈单亲家庭子女的学校教育

本文在对单亲家庭子女的性格作客观分析的基础上,探讨了学校教育管理中如何引导单亲家庭子女健康成长的问题。主要提出了学生管理中的五大沟通策略。     

基于单片机的煤气渗漏监控系统的设计

以8051单片机为核心，结合防爆型煤气浓度监测传感器及AD0832等，实现煤气化生产各环节中煤气渗漏的实时监测、采集、处理.     

A/D转换技术及其发展

为了适应A/D转换发展的要求,A/D转换技术也变得越来越复杂。就A/D转换这一领域所应用的主要转换技术以及各自的特点做一简要的介绍,并由此推断出A/D转换技术的发展趋势。