东方簇盾吸虫蛋白质和同工酶电泳研究

本文用浓度梯度电泳技术对东方簇盾吸虫(Lophotapis orientalis)的蛋白质进行了分析,测定了成虫糖蛋白、脂蛋白和纯蛋白的相对迁移率、分子量和相对含量;全蛋白共27条区带,分子量范围为10～300千道尔顿.同时,还对该吸虫的20种同工酶进行了电泳分析,根据18种同工酶的25个基因座位资料,计算出其多态性为0.68,群体平均杂合性为0.7209,说明东方簇盾吸虫自然群体中的遗传变异较大,具较大的进化潜力.     

侧柏容器育苗与造林技术浅探

侧柏,具有耐瘠薄、耐干旱,适应能力强,寿命长的特点,是造林绿化的主要树种。应对侧柏育苗造林技术有计划、有步骤地研究和探讨,并积极推广利用。     

中国对虾胚胎发育期卵的形态变化

通过对中国对虾胚胎发育时期卵的形态变化的观察，提出因受内分泌系统调节的启动作用，使卵的成熟、排卵、排精得到协调统一；精子入卵的时间是在卵的第二次成熟分裂中期，表现出第一极体位于受精膜之外，第二极体位于受精膜之内，与资料记载有差异。     

东方铁线莲种宗变异式样分析

运用形象化散点图和柱形图对东方铁线莲种宗的３个近缘种的变异式样进行了定量分析．结果表明，东方铁线莲在萼片形态、毛被及叶的形态等方面与其它２个近缘种显著不同，而粉绿铁线莲和黄花铁线莲则十分相似，仅在叶的裂片形态特征上存在着一定的差异．笔者同意Ｃ．Ｆ．Ｌｅｄｅｂｕｒ（１８４２）将黄花铁线莲作为粉绿铁线莲的变种处理的观点     

烟台海域发光细菌的分离鉴定

在烟台黄海海域所捕获的鱼的内脏和体表分离得到27株海洋发光细菌,经生理、营养和形态学的特性鉴定为三个种:Photobacterium Phosphoreum, Vibrio orientalis和V.splendidus biotype Ⅰ.其主要表型特征和已报道的相一致.根据文献,V.splendidus biotype Ⅰ属世界罕见,至今它是我国分离成功的第二株,V.prientalis,和V.splendidus biotype Ⅰ,此外,实验证明分布于整个黄海.     

泽泻ISSR反应体系的建立与优化

以四川泽泻为实验试材,采用改进的CTAB法提取了泽泻的高质量总DNA模板,克服了泽泻中所富含的酚类物质的影响.对泽泻ISSR反应体系中各个主要影响因子进行了优化和筛选,建立了标记位点清晰、稳定、重复性好、可用于泽泻ISSR-PCR分析的最适反应体系,它们是:25μL PCR反应体系中,10×Taq酶配套缓冲液,1U Taq DNA聚合酶,1.8~2.0 mmol/L MgC l2,100μmol/L dNTP,0.3μmol/L引物,DNA模板约10~20 ng,退火温度在52~60℃;为进一步研究泽泻居群的ISSR分子标记鉴别奠定了基础.     

东方弧菌SOD的分离纯化和特性研究

从东方弧菌５１８菌株的细胞中提取超氧化物歧化酶（ＳＯＤ）。先以硫酸铵分级沉淀取得粗酶，再经ＤＥ－５２，ＤＥＡＥ－纤维素柱层析，并以５－５００ｍｍｏｌ／Ｌ磷酸钾缓冲液作线性梯度洗脱，再次分级沉淀而获纯化的ＳＯＤ，呈棕黄色，其分子量为３８０００道尔顿，为二聚体，亚基分子量为１８５００道尔顿，原子吸收光谱测定其金属辅基为Ｆｅ＾＋＋＋。该酶反应最适温度为２５℃，室温下溶液中很易失活。紫外及可见光吸收光谱测     

树干注射技术防治芳香木蠹蛾的试验研究——药液的不同浓度防治不同树龄的试验

为有效防治芳香木蠹蛾,采用树干注射不同浓度40%氧化乐果乳油稀释液的方法,以不同树龄的稠李为实验对象,对芳香木蠹蛾1~3龄幼虫进行防治试验。经相互比较实验结果而知,在一定范围内(以对植株不产生伤害为基准),对于近似周径的稠李而言,浓度越大,防治效果越好;而同一浓度,相同的注射量,对于不同树龄而言,则周径越小,防治效果越好。     

苍耳螟寄生蜂种类调查及黄眶离缘姬蜂生物学特性研究

苍耳Xanthium sibirium属菊科,为一年生草本,是稻田周围常见杂草类寄主植物,在我国广有分布.作者从1992—1997年查明湖南省部分地区苍耳蛀虫种类和苍耳螟寄生蜂种类,考查五千多头幼虫,鉴定出四种蛀虫,17种寄生蜂,其中姬蜂7种,茧蜂7种,小蜂3种.黄眶离缘姬蜂数量较多,寄生率较高,可达19%.该蜂是苍耳螟幼虫的一种主要寄生蜂,并可寄生二化螟、三化螟、稻纵卷叶螟.进行了黄眶离缘姬蜂的生物学特性研究.     

Responses of woody plant root and xylem sap ATP to soil drying

The ATP in roots and xylem sap of two woody plant species, Platycladus orientalis and Acacia auriculiformis, subjected to rapid and slow soil drying has been determined employing firefly luciferase ATP assay method (sensitivity is at 10-12 mol ATP L-1). The ATP levels in the two species were 1.6 nmol·gDW-1 and 0.6 nmol·gDW-1 in roots, and 5.6 μmol·m-3 and 8 μmol ·m-3 in xylem sap, respectively. When plants of P. orientalis and A. auriculiformis were subjected to rapid soil drying, respectively, as soil water content (SWC) decreased from the normal level ( 0. 25 g ·gDW-1) to 0. 02 and 0. 06 g ·gDW-1, separately, plant water potential ( ) dropped to -4 and -3.2 MPa, differently, the ATP in roots decreased 99.7% and 42%, respectively . When the rapidly dried soil was watered for up to 6 d, SWC and were found to recover to their normal levels, but ATP content in roots of P. orientalis and A. auriculiformis recovered by 10% and 23%, respectively. When plants of P. orientalis and A. auriculiformis were subjected to slow soil drying, respectively, as SWC was found to decrease from the normal level to 0.1 and 0.13 g·gDW-1, separately, dropped to - 2.5 and - 2 MPa, differently, the ATP content in roots decreased 98% and 38%, respectively. When the slowly dried soil was watered for up to 8 d, ATP levels in roots of P. orientalis and A, auriculiformis recovered by 30% and 24%, respectively. However, ATP concentrations in xylem sap were not directly influenced by either SWC or . The plot dots of xylem sap ATP concentration versus root ATP content were found to be distributed diagonally. It is concluded that ATP in roots is more sensitive in response to SWC changes than that to and root-sourced ATP is a source of that in xylem sap. When plants are subjected to slow soil drying, the ATP levels in both root and xylem sap are higher than that to rapid soil drying.