北京棒杆菌天冬氨酸激酶突变体P184Q的酶学性质表征

通过分析谷氨酸棒杆菌天冬氨酸激酶(AK)的结构,筛选可能影响别构抑制剂结合的Pro184位点,对其进行饱和定点突变,成功筛选出突变菌株P184Q.酶学性质研究表明:突变体P184Q的V_(max)比野生型(WT)提高了3倍;n=1.39,低于WT的值(2.6),正协同性下降,同时Km值减小,对底物的亲和力增大;P184Q最适pH=6.5,最适反应温度为25℃,半衰期为2.8h;P184Q对金属离子和有机溶剂均表现出良好的抗性,解除抑制剂苏氨酸和甲硫氨酸、赖氨酸和甲硫氨酸、苏氨酸和赖氨酸、赖氨酸对酶活力的抑制作用.     

The new sideway of CNTF signal transduction pathway

The action of ciliary neurotrophic factor (CNTF) on intercellular free Ca²⁺ concentrations [Ca²⁺]_i induced by glutamate (Glu) in primary cultured hippocampal neurons were detected with Fura2/AM, a Ca²⁺-sensitive fluorophore, and the morphological influence of G-protein on it was objected. Glu could induce rapid increase of [Ca²⁺]_i in hippocampal neurons. CNTF had no significant action on [Ca²⁺]_i in resting hippocampal neurons. However, after incubation of CNTF for 5 min, the increase of [Ca²⁺]_i in hippocampal neurons rapidly induced by Glu was inhibited. Pretussis toxin (PTX)-sensitive G protein could block the action. These results indicate that a new non-genomic rapid sideway might exist in the upper stream of CNTF signal transduction pathway, which was related to Ca²⁺ signal transduction. Keywords:     

厌氧一微氧生物法处理味精生产废水的研究

采用热絮凝、汽提等方法对味精废水进行预处理,ＣＯＤ、ＮＨ２－Ｎ和ＳＯ２-4分别去除５０％、８０％和６０％以上．考察了ＵＡＳＢ反应器与ＣＳＴＲ光合反应器厌氧－微氧串联系统治理味精废水效果,在进料ＣＯＤ浓度为２５．６ｇ／Ｌ条件下,其ＣＯＤ处理能力达３．９ｋｇ／ｄｍ３,ＣＯＤ去除率达９７％．     

味精废水处理技术进展

从不同角度分类介绍了味精废水的治理技术和方法及其应用状况,具体包括理化预处理（高速离心、絮凝沉降、加热沉淀、超滤技术等）及生物处理（饲料酵母法、厌氧生物处理法、好氧生物处理法、藻-菌共生法、水解-好氧法等）。     

吸附伏安法测定N-月桂酰基谷氨酸钠的临界胶束浓度

用吸附伏安法测定了N-月桂酰基谷氨酸钠(LAG-Na)的临界胶束浓度(cmc),与用表面张力法和电导法比较,数据吻合,重现性好.     

味精生产排出的废液对白菜生长的影响

味精废液是一个污染源,但它又含有植物生长所需的营养物质,研究结果表明,味业认可促进白菜生长,增加叶的长度和宽度,增加白菜地上部分的鲜重和干重;提高叶片可溶性蛋白质含量、叶绿素含量和硝酸还原酶活性,但单位鲜重的可溶性糖含量低于对照;施用味精废液的白菜生产比不上施用复合肥的。     

Cholinergic, monoaminergic and glutamatergic changes following perinatal asphyxia in the rat

Perinatal asphyxia (PA) is considered to lead to a variety of brain disorders including spasticity, epilepsy, mental retardation, and minimal brain disorder syndromes and may form the basis for psychiatric and neurodegenerative diseases later in life. We examined markers for neuronal transmission involved in the pathomechanisms of PA and candidates as mediators for long-term sequelae. We tested tyrosine hydroxylase (TH) and the vesicular monoamine transporter (VMAT) representing the monoaminergic system, the vesicular acetylcholine transporter (VAChT), and the excitatory amino acid carrier 1 (EAAC1), a neuronal subtype of the glutamate transporter, using immunohistochemistry on brain sections of rats subjected to graded PA. Three months following the asphyxiant insult immunoreactive (IR)-TH was decreased in striatum, hippocampus, thalamus, frontal cortex, and cerebellum; IR-VMAT was increased, and IR-VAChT was decreased in striatum. IR-EAAC1 glutamate transporter was increased in frontal cortex. The cholinergic, monoaminergic, and glutamatergic changes, still observed 3 months after the asphyxiant insult, may reflect their involvement in the pathomechanisms of PA and indicate mechanisms leading to long-term complications of PA. The variable consequences on the individual markers in several brain regions may be explained by specific susceptibility of cholinergic, monoaminergic, and glutamatergic neurons to the asphyxiant insult. Received 16 March 1999; received after revision 20 May 1999; accepted 8 July 1999     

谷氨酸生产菌T6—13谷氨酸脱氢酶特性研究

本文对谷氨酸生产菌Te_(-13)谷氨酸脱氢酶的辅酶专一性、热稳定性、最适温度、最适pH、动力学参数和发酵过程中酶活力变化进行了研究。结果表明该酶属胞内酶,以NADP~+为专一性辅酶,脱氨反应的最适pH为9.5,酶的最适温度为35～40℃,酶的热稳定性试验表明50℃保温10分钟,残留活力为50%,60℃保温10分钟残留活力为4%,70℃下则完全丧失活力,对L—谷氨酸和NADP~+动力学参数分别为2.13×10.2mol/L和6.58×10~(-5)mol/L,AMP、ADP对脱氨反应有激活作用,ATP无影响。     

实验动物牛棒杆菌感染危害与防控措施

本文对棒状杆菌(Corynebacterium spp.)感染引起的过度角化症的流行病学特点、临床症状、诊断方法和防控净化措施的研究进展进行综述.结合啮齿类实验动物饲养管理特点,推荐有效的动物设施灭菌净化方法以及科学的预防控制措施.当动物设施被诊断出牛棒状杆菌(Corynebacterium bovis)感染,建议对饲...     

L-谷氨酸氧化酶产生菌理性化筛选及液体发酵的研究

采用HNO_2诱变、平皿显色与理性化筛选方法,获得一株L-谷氨酸氧化酶高产稳定菌株Streptomyces sp.N_514。建立了液体发酵产酶的条件,考察了辅加成分对酶合成的影响。