鼠标定位即时显隐导航栏的创建

网站传统导航模式的主要不足在于导航栏位置固定且占用版面,需要浏览者花费时间和精力寻找和调整导航栏,导航效果较差.在网页中嵌入JavaScript脚本,创建新式导航栏,使浏览者能够迅速、方便地控制导航栏的显示位置和显隐时机,可极大地改善导航效果,提高导航的实用性和便捷性.     

扩增引进限制性酶切位点技术检测CYP2C9基因的多态性

建立了一种简便、快速、准确的检测CYP2C9基因Arg144Cys位点和Ile359Leu位点多态性的新方法.该方法采用与模板具有1个碱基错配的引物通过PCR在扩增产物中引进限制性酶切位点,即扩增引进限制性酶切位点(amp lification-created restriction sites,ACRS),其中通过上游引物在扩增Arg144Cys位点的片断中引入AvaⅡ切点,使得野生型基因CYP2C9*1和突变型基因CYP2C9*2的PCR产物中分别含有2个和1个AvaⅡ切点;通过下游引物在扩增Ile359Leu位点的片断中引入MvaⅠ切点,使得野生型基因CYP2C9*1和突变型基因CYP2C9*3的PCR产物中分别含有1个和2个MvaⅠ切点.将来自Arg144Cys位点和Ile359Leu位点的PCR产物各取一份测序,表明错配碱基成功引入PCR产物中.将包含Arg144Cys位点的PCR产物用AvaⅡ消化,将包含Ile359Leu位点的PCR产物用MvaⅠ消化,然后通过聚丙烯酰胺凝胶电泳-银染进行基因分型.由于野生型基因和突变型基因的PCR产物中都至少含有1个酶切位点,能完全避免因限制性核酸内切酶失活或酶切不完全而导致的基因分型错误.     

关于建筑节能技术发展的探讨

节能是国内住宅建设的重中之重,根据墙改的要求,建筑节能技术在我国已经有了一定的发展.本文介绍了建筑节能技术内容,并分析了采暖居住建筑的节能重点部位和节能途径,得出窗户是节能的重点部位.     

Cholera toxin structure, gene regulation and pathophysiological and immunological aspects

Many notions regarding the function, structure and regulation of cholera toxin expression have remained essentially unaltered in the last 15 years. At the same time, recent findings have generated additional perspectives. For example, the cholera toxin genes are now known to be carried by a non-lytic bacteriophage, a previously unsuspected condition. Understanding of how the expression of cholera toxin genes is controlled by the bacterium at the molecular level has advanced significantly and relationships with cell-density-associated (quorum-sensing) responses have recently been discovered. Regarding the cell intoxication process, the mode of entry and intracellular transport of cholera toxin are becoming clearer. In the immunological field, the strong oral immunogenicity of the non-toxic B subunit of cholera toxin (CTB) has been exploited in the development of a now widely licensed oral cholera vaccine. Additionally, CTB has been shown to induce tolerance against co-administered (linked) foreign antigens in some autoimmune and allergic diseases. Received 25 October 2007; accepted 12 December 2007     

Web日志挖掘中重构网站结构技术

提出了一种重构网站结构的方法,并将此方法应用于Web日志挖掘的数据预处理中．实验表明,本方法能够更好地还原用户访问情景,为Web日志挖掘提供有效数据。     

浅谈八泉峡水库坝址工程地质特征

八泉峡水库坝址处于八道水河上,拟建坝型为混凝土重力坝。对坝址的工程地质特征及主要存在问题进行了论述和分析,并提出了合理评价。     

Enhancing site style tree construction algorithm

The World Wide Web has become a global information service center with a vast amount of news, advertisements, product and service information, and disparate information from diversified sources. However, only a small portion of information is truly relevant and useful to the users who are seeking information on specific topics. In this paper, common relations among nodes are taken into consideration when constructing site style tree, and a new node type is introduced. Experimental results show that the proposed algorithm has higher precision and recall.      

建筑施工现场管理存在的问题及对策

建筑施工现场管理是工程项目管理的核心,也是确保建筑工程质量和安全文明施工的关键。对施工现场实施科学的管理,是树立企业形象、提高企业声誉、获取经济效益和社会效益的根本途径。文章主要分析了影响建筑施工现场管理的因素,并针对存在的问题提出了提高现场施工管理的对策。