螺旋藻_S_platensis_S_maxima_培养液的筛选

通过用粗海盐取代Ｚａｒｒｏｕｋ培养液中部分微量元素,得到钝顶螺旋藻（Ｓ．ｐｌａｔｅｎｓｉｓ）和巨大螺旋藻（Ｓ．ｍａｘｉｍａ）的简化培养基：用１．０ｇ／Ｌ粗海盐代替Ｚａｒｒｏｕｋ培养液中Ａ＿５和Ｂ＿６液成分,ＭｇＳＯ３·７Ｈ２Ｏ剂量为０．２５ｇ／Ｌ,其它成分同Ｚａｒｒｏｕｋ培养液．     

Genetic and morphometric variation in the potamonautid river crab Potamonautes parvispina (Decapoda: Potamonautidae) from two Western Cape rivers,South Africa

Patterns of genetic and morphometric variation amongst five populations of Potamonautes parvispina from two rivers in the Western Cape, were examined. Genetic variation at 14 loci was investigated using protein gel electrophoresis. The results showed that the populations were genetically invariant within and between the two drainage systems. Discriminant functions analyses of seven carapace variables demonstrated regional differentiation between the population groups in the two drainage systems. Other morphometric characters exhibited a significant degree of variation within drainage systems. Morphometric data were highly variable and were discordant with the genetic data, which suggests that environmental factors may be responsible for the large degree of phenotypic plasticity. Results from the present study are discussed in terms of widespread gene flow and recent population divergence.     

Diagrams and alien ways of thinking

The recent wave of data on exoplanets lends support to METI ventures (Messaging to Extra-Terrestrial Intelligence), insofar as the more exoplanets we find, the more likely it is that “exominds” await our messages. Yet, despite these astronomical advances, there are presently no well-confirmed tests against which to check the design of interstellar messages. In the meantime, the best we can do is distance ourselves from terracentric assumptions. There is no reason, for example, to assume that all inferential abilities are language-like. With that in mind, I argue that logical reasoning does not have to be couched in symbolic notation. In diagrammatic reasoning, inferences are underwritten, not by rules, but by transformations of self-same qualitative signs. I use the Existential Graphs of C. S. Peirce to show this. Since diagrams are less dependent on convention and might even be generalized to cover non-visual senses, I argue that METI researchers should add some form of diagrammatic representations to their repertoire. Doing so can shed light, not just on alien minds, but on the deepest structures of reasoning itself.     

First record of the genus Leptodactylus (Anura: Leptodactylidae) in Cuba: Leptodactylus fragilis,a biological invasion?

The Neotropical genus Leptodactylus is currently represented by three species in the West Indies (Leptodactylus albilabris, Leptodactylus fallax and Leptodactylus validus). Based on morphological, acoustic and molecular evidence, we document the presence of a fourth species in the Caribbean region, Leptodactylus fragilis (Brocchi, 1877). The species was found at two localities in western Cuba, and molecular data suggest a northern South American origin, possibly Venezuela, for these populations. We discuss the potential invasive status of L. fragilis, based on its known distribution, relative abundance, behaviour and possible impacts on native species of Cuban amphibians.     

科学技术与社会(STS)研究热点和发展趋势之管窥——基于质性数据分析的4S年会2016-2020年综述

回顾了国内4S年会综述和STS研究综述文献,以4S年会会议资料和中国科协创新战略研究院的参会报告为研究资料,运用质性数据分析方法对年会主题、分论坛议题和演讲摘要进行了统计研究,分析了STS研究热点和发展趋势,评述了2016-2020年4S年会概况,验证了STS全球发展趋势和议题扩散态势,并对国内STS研究与参加4S年会...     

美国国防创新小组对中国军民科技协同创新发展的启示

分析了美国国防创新小组(DIU)的设立背景、演变过程和主要举措,探讨了中国军民科技协同创新发展中存在的问题,从企业合作、规划制定、二次创新、关注领域等方面提出了未来发展的启示。     

沪深股票市场长程记忆相关性研究

针对上海和深圳的日收益序列,采用重标级差(R/S分析)对其进行了实证研究.从统计结果来看,样本序列呈现出尖峰、胖尾等有偏特征,明显不满足正态分布的假设,表明收益序列可能具有长程相关或记忆性.采用ARFIMA模型对沪深股市收益率的长期记忆性进行了检验,根据分段检验的结果,得出了一些我国证券市场有效性的结论.     

一种新型天蚕素杂合肽AD和aFGF突变体融合蛋白S的构建及表达

目的：构建和表达一种带有凝血酶切割位点的天蚕素杂合肽AD和人酸性成纤维细胞生长因子(acidic fibroblast growth factor,aFGF)突变体的融合蛋白S,在创伤皮肤表面应用时能自动裂解成有抗感染功能的天蚕素杂合肽AD和皮肤修复功能的aFGF突变体。方法：利用P(R技术分别扩增出带有凝血酶切割位点的天蚕素杂合肽AD基因和aFGF突变体基因。再利用两作为模板,用PCR方法扩增出编码融合蛋白S的DNA。将该DNA片段插入到载体pPICZaA中,并利用电转法将质粒转入毕赤酵母smd1168中,Zeocin抗性筛选重组转化子。甲醇诱导72h,SDS-PAGE电泳检测融合蛋白S的表达,并用Western-blot杂交检测融合蛋白S的免疫原性。结果：筛选出的重组转化株在甲醇诱导后能表达相对分子质量约为19000的融合蛋白S,而该蛋白能与aFGF抗体产生免疫反应。结论：用PCR方法获得编码含凝血酶切割位点的天蚕素杂合肽AD和aFGF突变体的融合蛋白SDNA,并在毕赤酵母中实现了表达。     

广义逆A(2)T,S的体积

研究了广义逆A（T,S）^（2）是的体积,在不必首先计算出广义逆A（T,S）^（2）的前提下,导出了广义逆A（T,S）^（2）的体积表示,推广了文献[3]中的结果．由此分别给出了A的加权Moore-Penrose逆,Drazin逆Ad及群逆Ag的体积表示式．     

转糖基β-半乳糖苷酶产生菌筛选和鉴定及酶催化生成低聚半乳糖

通过水解活性和转糖基活性筛选, 从实验室97株保存菌种中获得1株具有转糖基活性的β-半乳糖苷酶产生菌,克隆并序列分析了该菌株16SrDNA基因片断,GenBank收录号为DQ267829. 综合其形态学特征、生理生化特征及16S rDNA序列同源性分析结果, 将其鉴定为巨大芽孢杆菌（Bacillus megaterium）2-37-4-1. 确定了该菌株β-半乳糖苷酶产酶培养基的碳源为乳糖1%,氮源为蛋白胨0.5%和酵母膏0.5%,培养条件为37℃摇床培养18?h;碳源实验证明,该菌株β 半乳糖苷酶产生为乳糖诱导型.利用薄层层析技术研究了pH值、乳糖底物浓度、反应温度和反应时间对该菌株β-半乳糖苷酶以乳糖为底物转糖基合成低聚半乳糖的影响, 确定最适反应条件为pH7.5、50mmol/L（磷酸缓冲液）配制的40%乳糖溶液, 55℃反应24h.转糖基反应产物高压液相色谱分析其组成为低聚半乳糖25.68%, 双糖（包括乳糖和转移二糖）33.02%, 葡萄糖26.37%和半乳糖14.92%.