两株细菌对偶氮染料活性艳红X—3B脱色的研究

从太原市纺织绒染厂废水处理站曝气池分离筛选到一株假单胞菌和一株黄单胞菌,探讨了这两株菌在不同碳源、不同ｐＨ和不同温度条件下对活性艳红Ｘ—３Ｂ的脱色效果。试验结果表明,假单胞菌和黄单胞菌分别以葡萄糖,果糖和无水乙酸钠为碳源时,脱色效果都较好,且黄单胞菌以可溶性淀粉作碳源时也有较高的脱色率。这两株菌脱色的最适ｐＨ均为８．０。假单胞菌脱色的最适温度为２５℃,黄单胞菌脱色的最适温度为３０℃。     

all 1691基因表达及铁元素对鱼腥藻生长的影响

为实现FurA的表达,探究铁元素对藻生长的影响,首先运用Primer5.0设计引物,克隆出鱼腥藻PCC7120染色体上all1691(furA)基因片段;构建含组氨酸融合表达标签和T7启动子标签的表达载体.IPTG诱导FurA蛋白表达.然后设计不同Fe3+浓度梯度培养基,利用SDS-PAGE检测高、中、低Fe3+浓度培养液的藻细胞总蛋白,考马斯亮蓝g-250法测定蛋白含量,并用Trizol法提取不同Fe3+浓度培养的藻细胞总RNA.结果表明,获得纯化的all 1691克隆片段,大小为456bp,pET-1691重组蛋白在1mmol/L的IPTG诱导下,于37℃下摇床培养15h得到成功表达;低浓度Fe3+促进藻生长,高浓度Fe3+抑制藻生长;Fe3+浓度为2.0mg/L时rRNA位置与亮度清晰可见,而缺铁培养的藻生长几乎停滞,转录和翻译水平都很低.     

短指软珊瑚Sinularia sp.内生真菌Ta31-2代谢产物研究(Ⅱ)

从短指软珊瑚Sinularia sp.内生真菌Ta31-2的代谢产物中分离得到10个化合物,利用波谱技术确定其结构分别为硬脂酸(1)、软脂酸(2)、肉豆蔻酸(3)、癸酸甲酯(4)、麦角甾醇(5)、fusanolide B(6)、丁二酸(7)、curvulalide(8)、modiolide A(9)和neoechinulin A(10)。其中,fusanolide B(6)为首次从海洋真菌中分离得到,curvulalide(8)和neoechinulin A(10)为首次从软珊瑚Sinularia sp.内生真菌中分离得到。     

球孢白僵菌产几丁质酶液态发酵研究

对球孢白僵菌Bb174产几丁质酶的液态发酵条件进行了试验研究。选择麸皮和蚕蛹粉作为培养基的基本成分。以不同比例的麸皮和蚕蛹粉进行试验,结果显示:麸皮和蚕蛹粉分别为5％,培养温度28℃,接种量10％,装液量10mL,起始pH6.0,添加0.3％NaNO_3对产儿丁质酶有利,最高酶活力为4664U/mL。     

环境因子对青海弧菌生长和发光的影响

该文采用了多因子正交试验与单因子试验相结合的方法研究了环境因子对青海弧菌性长和发光的影响,发现各因子对青海弧菌的生长和发光的影响程度是不同的。其中甘油对生长的影响最大,而对发光的影响最大的却是氮源。根据各因子的影响情况,本文提出了有利于青海弧菌生长和发光的致好的培养基配方及培养的温度条件,并试着对环境因子对青海弧菌生长和发光的影响机制作了一些讨论。     

红假单胞菌产类胡萝卜素条件的研究

通过对影响红假单菌类胡萝卜素的因素的全面研究,获得了胡萝卜素产量相对辊主贩培养条件,以乙酸钠为碳源,谷氨酸钠为氮源,酵母亮为生长因子,４ｍｇ／Ｌ,ＭｇＳＯ４,２ｍｇ／ＬｆＥ２（ｓｏ４）３,１５００ｌｘ光照度,３０℃,微好氧培养１２０ｈ。     

Effect of monoclonal antibody to bull sperm sp18 on murine fertilization and embryos development

Western blot analysis revealed that one IgG1 monoclonal antibody (mAb) to sp18 family membrane proteins (Mr. 14, 16 and 18 ku) of bovine sperm reacted faintly with protein bands of 14, 18, 22, 30 and 60 ku (reducing) in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence (IIP) showed that the sp18 antigens were present in the posterior head of murine sperm. In murine in vitro fertilization (IVF) and embryo development trails, a total of 426 oocytes from C57BL/6 and F1 hybrid strain (CD1 × C57BL/6 cross) of 12 female mice were used in 3 independent trails. After preincubating capacitated sperm with 182 μg/mL of sp18 mAb in the modified TYH IVF medium for 15-20 min, cumulus-oocyte-complexes were introduced. The fertilization rate in sp18 mAb groups was 77.1 %, which was not significantly (P > 0.05) different from the nonspecific mouse IgG (79.2%) and non-IgG (80.3 %) control groups. Fertilized oocytes had been continuously cultured in modifed CZB medium. 100%, 100% and 97.9% of 1-cell embryos developed to 2-cell stage in sp18 mAb, nonspecific mouse IgG and non-IgG group 30 h after the start of fertilization, respectively. In the nonspecific mouse IgG and non-IgG groups, 64.1 % and 64.3% of embryos developed to the 4-cell stage, respectively, but all developing eggs in sp18 mAb groups arrested development in vitro at 2-cell stage. After zonae of 2-cell blocked embryos were enzy-matically removed with 0.5% pronase, detection of sp18 antigens by IIF indicated that the fluorescence scattered on two embryonic cells. For embryos fertilized in vivo and co-cultured with 182 μg/mL sp18 mAb, the numbers of 1-cell embryos reaching the 2-cell and 4-cell stage were 95. 2% and 70. 5%, which were not significantly (P>0.05) different from the control group (92.9% and 77.9%). These results indicate that the sp18 antigens on posterior head of mouse sperm were incorporated into the egg plasma membrane during fertilization, and played an active role in development of murine preimplanta-tion embryo.     

Pb2 胁迫对蛋白核小球藻叶绿素荧光强度及亚显微结构的影响

实验研究了蛋白核小球藻叶绿素荧光强度及亚显微结构受不同浓度Pb2+的影响。实验结果表明:随暴露时间的进行0、1 mg/L Pb(NO3)2处理组蛋白核小球藻的叶绿素荧光强度是逐渐升高的;而5、10、50、80 mg/L处理组的藻叶绿素荧光强度逐渐降低;且Pb2+浓度越大其荧光强度的下降趋势越明显。经Pb2+处理的蛋白核小球藻细胞易破裂且多变形,细胞壁和原生质体结合疏松,细胞超微结构中的细胞器如叶绿体、线粒体、蛋白核、淀粉与液泡等受到了Pb2+毒性影响,导致其细胞内的各个系统受到明显影响,进而影响到藻细胞的荧光特性。     

脉孢霉纤溶酶的酶学性质研究

研究了脉孢霉纤溶酶的部分酶学性质,确定该酶由双亚基组成,亚基分子量分别为30000Da、15500Da,等电点为7.9±0.2,最适作用pH值为7.4,最适作用温度为50℃,在pH6.6～9和32～42℃时较稳定。     

海洋来源真菌的曲酸发酵条件优化

为提高真菌Aspergillus sp.GXIMD02003的曲酸产量,本研究对其利用大米固体培养基发酵的条件进行优化,旨在获得成本低廉的曲酸原料,促进曲酸的工业化生产。研究采用单因素控制变量法考察最佳盐度和最佳发酵时间,通过HPLC法测定曲酸的产量。结果表明真菌Aspergillus sp.GXIMD02003产曲酸的最佳发酵条件为在2%海盐的大米固体培养基中发酵30 d,在此条件下1 000 g大米培养基能够发酵产生24.2 g曲酸。因此,海洋来源真菌Aspergillus sp.GXIMD02003可以作为曲酸的生产菌株,海盐浓度影响该菌株的曲酸代谢。