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81.
为了探究新型小分子抑制剂Napabucasin对结直肠癌细胞增殖以及迁移的影响. 首先通过分子模拟对接分析了Napabucasin与STAT3蛋白的互作机制. 然后利用克隆形成实验、细胞划痕实验等方法在多种结直肠癌细胞系中证明了Napabucasin能够显著抑制结直肠癌细胞的集落形成能力以及迁移能力. 进而使用Napabucasin与Wnt信号通路激活剂Wnt agonist 1共处理结直肠癌细胞HCT116,结合蛋白质印迹实验发现,Wnt信号通路介导了Napabucasin对结直肠癌细胞的迁移以及增殖的抑制过程. 研究结果显示,Napabucasin能够在体外抑制结直肠癌细胞的增殖能力以及迁移能力,并且Wnt信号通路参与介导了这一抑制过程. 相似文献
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Throughout growth and development,Dictyostelium cells secrete autocrine factors that accumulate in proportion to cell density. At sufficient concentration, these factors cause changes in gene expression. VegetativeDictyostelium cells continuously secrete prestarvation factor (PSF). The bacteria upon which the cells feed inhibit their response to PSF, allowing the cells to monitor their own density in relation to that of their food supply. At high PSF/bacteria ratios, which occur during late exponential growth, PSF induces the expression of several genes whose products are needed for cell aggregation. When the food supply has been depleted, PSF production declines, and a second density-sensing pathway is activated. Starving cells secrete conditioned medium factor (CMF), a glycoprotein of Mr 80 kDa that is essential for the development of differentiated cell types. Antisense mutagenesis has shown that cells lacking CMF cannot aggregate, and preliminary data suggest that CMF regulates cAMP signal transduction. Calculations indicate that a mechanism of simultaneously secreting and recognizing a signal molecule, as used byDictyostelium to monitor cell density, could also be used to determine the total number of cells in a tissue. 相似文献
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的探讨新生大鼠海马神经干细胞(NSC)的体外培养和诱导分化的条件和特点。方法分离出生1d大鼠海马,在表皮生长因子、碱性成纤维生长因子和B27联合作用下使其稳定增殖,用5-溴脱氧尿苷(BMU)标记处于增殖状态的神经干细胞,应用免疫荧光染色方法行巢蛋白(Nestin)、5-溴脱氧尿苷(BrdU)、β-Ⅲ型微管蛋白(Tuj-1)、波形蛋白(Vimenfin)和Galc-C免疫荧光染色,对NSC的增殖及其分化的细胞进行鉴定。结果体外培养的NSC增殖成神经干细胞球并传代,鉴定为Nestin染色阳性细胞和5-溴脱氧尿苷(BrdU)标记染色阳性细胞,并可诱导分化为神经元细胞(Tuj-1染色阳性细胞)、神经胶质细胞(Vimentin染色阳性细胞)和少突胶质细胞(GMc-C染色阳性细胞)。结论采用无血清培养基中加入特定生长因子的培养技术,可培养出在体外稳定增殖并有多向分化潜能的新生大鼠海马神经干细胞。 相似文献
87.
Glycine-rich proteins as structural components of plant cell walls 总被引:12,自引:0,他引:12
Glycine-rich proteins (GRPs) have been found in the cell walls of many higher plants and form a third group of structural
protein components of the wall in addition to extensins and proline-rich proteins. The primary sequences of GRPs contain more
than 60% glycine. GRPs are localized mainly in the vascular tissue of the plant, and their coding genes provide an excellent
system to analyze the molecular basis of vascular-specific gene expression. In French bean, the major cell wall GRP has been
localized at the ultrastructural level in the modified primary cell wall of protoxylem. Immunological studies showed that
it forms a major part of these highly extensible and specialized cell walls. Specific digestion of GRP1.8 from bean by collagenase
suggests that it shares structural similarities with collagen. The protein is synthesized by living protoxylem cells as well
as xylem parenchyma cells. After cell death, GRPs are exported from neighboring xylem parenchyma cells to the protoxylem wall,
a rare example of protein transport between cells in plants. We propose that GRPs are part of a repair system of the plant
during the stretching phase of protoxylem. 相似文献
88.
对鼠腹腔中弓形虫速殖子的增殖发育的方式、过程及S.D.对其的影响作了详细的考察,结果表明弓形虫的无性生殖方式有内二裂殖、纵二裂殖、裂殖生殖等,虫体以鼠腹腔内巨噬细胞的胞壁作为假包囊壁有其中增殖发育,经过72h的增殖生长,虫体成熟后突破假包囊逸出,S.D.可部分抑制速殖子增殖。 相似文献
89.
Plasmin(ogen)wasknownforitsfibrinolyticactivityaswellasitsω aminocarboxylicacid bindingability.Onehighand 4low affinitybindingsitesweredetectedinplasminogenheavychain .Concur rently 5highlyhomologoustripleloopstructuresnamedaskringledomainswerefoundforming… 相似文献
90.
瞬态电磁脉冲对细胞增殖的影响 总被引:3,自引:0,他引:3
实验采用有丝分裂指数测定法及^3H-TdR掺入法,研究瞬态电磁脉冲对人外周血淋巴细胞及艾氏腹水癌细胞增殖的影响。结果发现,没有植物凝集素PHA时,100kHz和200kHz瞬态电磁脉冲对淋巴细胞有丝分裂指数和^3H-TdR掺入量无显著影响;有PHA时,100kHz瞬态电磁脉冲促进淋巴细胞摄取^3H-TdR,细胞有丝分裂指数增加,200kHz瞬态电磁脉冲抑制淋巴细胞摄取^3H-TdR,细胞有丝分裂指 相似文献