MTMR14稳定敲减细胞株的建立及表型分析

目的: 为了解析芽殖酵母 Num1 蛋白在纺锤体定位和线粒体中的调节机制．方法: 利用大肠杆菌原核表达 并纯化了在 Num1 功能中起核心作用的补丁组装(PA) 结构域的重组蛋白, 通过蛋白体外结合实验(Pull-down) 分 离了酵母细胞中与 PA 结构域相结合的蛋白复合物,并对其进行了质谱分析．结果: 鉴定了一系列新的 Num1 互作蛋 白,包括核糖体蛋白,参与蛋白折叠、分配及转运的内质网和高尔基复合体蛋白, 参与基因转录和翻译的核酸酶和 蛋白酶,及其他功能的蛋白．结论: Num1 的互作蛋白的鉴定为进一步研究 Num1 的作用机制奠定了基础．     

1,25(OH)2D3对卵巢癌细胞HO8910生长的抑制作用

为探讨1,25-二羟维生素D3[1,25-dihydroxy vitamin D3,1,25(OH)2D3]对人卵巢癌细胞HO8910生长和增殖的作用,选用人卵巢癌细胞HO8910进行体外培养。培养时添加不同浓度的1,25-二羟维生素D3,采用MTT比色法测定细胞生长抑制率,采用流式细胞术(flow cytometry,FCM)进行细胞周期及细胞凋亡分析。结果:不同浓度的1,25-二羟维生素D3对HO8910细胞生长均起到不同程度的抑制作用(P<0.05),并呈浓度和时间依赖关系;能使细胞周期时相发生改变,G1期细胞增多(P相似文献   

机箱屏蔽设计和屏蔽效果测量

分析了机箱产生电磁泄漏的机理,介绍了机箱电磁屏蔽的设计方法和利用GHz横电磁波室(GTEM Cell)测量机箱屏蔽效果的方法.在GTEM Cell内对采取不同屏蔽措施机箱的屏蔽效果进行了测量,给出了定量的数据.为提高机箱的屏蔽性能,提供了理论依据和实验数据.     

姜黄素对人膀胱癌细胞系T24体外侵袭的作用

目的探讨姜黄素(curcumin)对人膀胱癌细胞系T24体外侵袭能力及其作用机制.方法以不同浓度的姜黄素作用于膀胱癌T24细胞,MTT法检测细胞增殖抑制率,Transwell法实验观察T24侵袭能力的变化,Western blot法检测MMP-2和VEGF蛋白表达的差异.结果姜黄素能有效抑制T24细胞的增殖,并抑制T24细胞的体外侵袭能力,呈时间-剂量依赖性(P0.05).20μmol/L和50μmol/L姜黄素作用细胞24 h后,MMP-2,VEGF蛋白的表达量下降(P0.05).结论姜黄素对T24细胞的增殖和侵袭能力有抑制作用,其机制可能与下调MMP-2和VEGF的蛋白表达有关.     

影响螺旋藻生物量的因素研究进展

影响螺旋藻生物量的因素作了比较全面的综述，碳源、氮源、光照、温度、pH值、循环速度、溶氧浓度、磁场、激光、各种植物生长调节剂、环境有机毒物等因素对螺旋藻的生物量积累有显著的影响。     

绿茶多酚表没食子儿茶素没食子酸酯对肝癌Hep G2细胞的增殖抑制作用和凋亡诱导效应

表没食子儿茶素-3-没食子酸酯((-)-epigallocatechin-3-gallate,EGCG)是绿茶多酚的主要成分,具有抗氧化、抗衰老、抗过敏和潜在的抗癌特性.本文运用MTT试验,DAPI染色和Annexin V-FITC分析了EGCG对肝癌Hep G2细胞的增殖抑制作用和凋亡诱导.结果表明,EGCG对Hep G2细胞具有明显的增殖抑制作用,存在剂量和时间依赖效应;EGCG能诱导Hep G2细胞凋亡,并具有剂量依赖效应.     

Intracellular protein degradation in mammalian cells: recent developments

In higher organisms, dietary proteins are broken down into amino acids within the digestive tract but outside the cells, which incorporate the resulting amino acids into their metabolism. However, under certain conditions, an organism loses more nitrogen than is assimilated in the diet. This additional loss was found in the past century to come from intracellular proteins and started an intensive research that produced an enormous expansion of the field and a dispersed literature. Therefore, our purpose is to provide an updated summary of the current knowledge on the proteolytic machinery involved in intracellular protein degradation and its physiological and pathological relevance, especially addressed to newcomers in the field who may find further details in more specialized reviews. However, even providing a general overview, this is an extremely wide field and, therefore, we mainly focus on mammalian cells, while other cells will be mentioned only for comparison purposes.     

Scale length/wing length correlation in Lepidoptera (Insecta)

Significant positive correlation between wing length and scale length was found in a broad sample of lepidopteran taxa. No breakdown of the correlation was observed in the lowest wing size range. The best fit is a non-linear (power) curve. The minimum individual scale length observed was 40 µm (in a nepticulid), while the maximum was close to 500 µm (in a castniid; there are literature records of even larger scales in this family). Scale size variation probably reflects variation in the size of scale-forming trichogen cells. The latter, in turn, may at least partly reflect difference in genome size, such as has long been known to be the case with the cells that form, respectively, the cover and ground scales in some higher moths.     

杨树木材细胞腔径分布的分形表征

应用杨树木材横切面的细胞直径分布描述木材横切面空隙分布的分形特征。通过对不同高度处木材细胞腔直径分布进行分析,分别计算出它们的分形维数(D=124～131)。结果表明:腔径分布分形维数的大小可以作为表征木材多孔性的一个参数。     

藏灵菇菌增殖条件及其乳酸发酵特性的研究

对藏灵菇菌增殖条件及其产乳酸特性进行了研究。藏灵菇增殖适宜条件为：乳浓度60%,培养温度28℃,接种量5%,pH值6.6,250 mL三角瓶装液量50 mL,转数100 r·m in^-1。连续培养5代后,其增殖率达到108%。藏灵菇产酸能力较强,培养20 h,pH值迅速下降到4.62,酸度增至109°T。外加碳源不利于藏灵菇生长,而添加蛋白胨和酵母浸提物则对藏灵菇增殖有促进作用。