地方师范院校生物技术专业《细胞工程》实验教学的探讨

21世纪生物技术高速发展，而地方师范院校的生物技术专业的学生就业前景不容乐观，培养高素质的实用型和创造型人才是地方师范院校生物技术专业发展面临的重要课题．本文从“细胞工程”课程的实验教学的角度，探讨生物技术专业实验教学方式的改进，为提高实践教学的水平提供理论基础．     

Antizyme and antizyme inhibitor, a regulatory tango

The polyamines are small basic molecules essential for cellular proliferation and viability. An autoregulatory circuit that responds to the intracellular level of polyamines regulates their production. In the center of this circuit is a family of small proteins termed antizymes. Antizymes are themselves regulated at the translational level by the level of polyamines. Antizymes bind ornithine decarboxylase (ODC) subunits and target them to ubiquitin-independent degradation by the 26S proteasome. In addition, antizymes inhibit polyamine transport across the plasma membrane via an as yet unresolved mechanism. Antizymes may also interact with and target degradation of other growth-regulating proteins. An inactive ODC-related protein termed antizyme inhibitor regulates polyamine metabolism by negating antizyme functions. The ability of antizymes to degrade ODC, inhibit polyamine uptake and consequently suppress cellular proliferation suggests that they act as tumor suppressors, while the ability of antizyme inhibitors to negate antizyme function indicates their growth-promoting and oncogenic potential.     

口服8种细菌溶解物治疗儿童反复呼吸道感染免疫指标的变化

目的观察8种细菌溶解物（泛福舒）治疗反复呼吸道感染前后免疫指标的变化。方法将117例反复呼吸道感染儿童121服泛福舒，每日1次，每次3．5mg，连用10d，停20d，3月为疗程。结果治疗前后免疫指标改变有显著性差异（P〈0．01）。结论口服泛福舒可提高儿童体液免疫和细胞免疫，可作为治疗儿童反复呼吸道感染的有效方法。     

Effects ofPKCα antisense RNA on human breast cancer cell proliferation and expression ofcyclinD1 andCDK4

The role of PKCα in human breast cancer cell proliferation and expression ofcyclinD1 andCDK4 has been investigated using inhibition ofPKCα expression by its antisense RNA. WhenPKCα expression was inhibited the rate of cell proliferation decreased apparently and the levels ofcyclinD1 andCDK4 mRNA were lower than the control. The results showed thatPKCα, a key member of signal transduction system, played an important role in human breast cancer cell proliferation and had a close relationship with expression ofcyclinD1 andCDK4 which control start of cell cycle.     

运用判别分析对肝癌及癌前病变形态定量研究初探

本文在运用判别分析方法对肝癌及癌前病变形态进行定量研究方面作了初步尝试,给出了具体的判别函数和判别规则.对原48个样品进行回报,其准确率达到81%以上.     

硫化氢对高压电机线圈电晕影响的试验研究

为解决西藏羊八井地热电站有害气体硫化氢对高压电机线圈电晕的影响,作者负责的课题组用发电机小样棒在通入硫化氢气体的人工气候室内做了模拟试验,继而到羊八井作实地调查及试验,提出并验证了起晕电压随硫化氢气体浓度增加而降低的关系,给了经验公式。对地热电站用发电机如何解决有害气体硫化氢的危害问题提出了建议。     

大鼠肝细胞系的建立及其特性研究

从 Wistar 新生大鼠肝组织已成功获得正常肝细胞体外长期培养细胞系 NRL-84.细胞生长情况、分裂指数、染色体分析、软琼脂集落形成试验、相差显微镜及扫描电镜等多项指标观察.表明符合正常肝细胞生物学特征;用间接免疫荧光、对流免疫电泳、核酸原位杂交等检测甲胎蛋白,符合正常肝细胞的变动规律.正常新生大鼠的建立是国内首次报导,它将为进一步研究恶性肿瘤的发生发展机制提供了一个良好的实验模型和手段.     

dentonin对牙髓干细胞增殖的实验研究

目的研究dentonin促进牙髓干细胞增殖,获得有关dentonin对牙髓干细胞增殖的实验证据。方法把构建好的含有真核细胞表达质粒pc DNA-dentonin,在脂质体Lipofect AMINE介导下,转染到体外培养的牙髓干细胞,再进行MTT检测细胞增殖。结果发现dentonin对牙髓干细胞生长有明显的增殖作用。结论牙髓干细胞生长速度较快,但是在两周后进入平台期,牙髓干细胞生长速度减慢。     

DCN调控TGF-βRII高表达抑制HepG2细胞增殖分子机制研究

目的 探讨DCN通过TGF-β信号通路抑制肿瘤细胞增殖的分子机制.方法 应用质粒转染方法诱导HepG2细胞高表达核心蛋白聚糖,应用siRNA法抑制核心蛋白聚糖表达,应用Western blot法检测HepG细胞TGF-β信号通路相关因子表达,最终用MTT法检测细胞增殖.结果 DCN通过增高TGF-βRII的表达,引起TGF-βRI磷酸化程度增高,诱导p15蛋白表达增高,与对照组比较,差异具有统计学意义(P<0.05).结论 DCN最终抑制HepG2细胞的增殖;使用siRNA沉默TGF-βRII可减弱DCN对HepG2细胞增殖的抑制作用.