Molecular basis of morphogenesis during vertebrate gastrulation

Gastrulation is a crucial step in early embryogenesis. During gastrulation, a set of morphogenetic processes takes place leading to the establishment of the basic body plan and formation of primary germ layers. A rich body of knowledge about these morphogenetic processes has been accumulated over decades. The understanding of the molecular mechanism that controls the complex cell movement and inductive processes during gastrulation remains a challenge. Substantial progress has been made recently to identify and characterize pathways and molecules implicated in the modulation of morphogenesis during vertebrate gastrulation. Here, we summarize recent findings in the analysis of signaling pathways implicated in gastrulation movements, with the aim to generalize the basic molecular principles of vertebrate morphogenesis.     

重组hFGF-7腺病毒对角质形成细胞的生物学效应

目的:研究重组腺病毒导入人成纤维细胞生长因子7(hFGF-7)对人皮肤角质形成细胞(HaCat)的生物学效应.方法:通过倍比稀释和感染实验,测定重组腺病毒rAd-hFGF-7的滴度;流式细胞术检测重组腺病毒感染HaCat细胞的感染效率;MTT法检测rAd-hFGF-7对HaCat细胞增殖的影响;重组腺病毒对HaCat细胞周期的影响通过流式细胞术进行检测.结果:高滴度重组腺病毒可高效感染HaCat细胞,其促细胞增殖作用随着重组腺病毒MOI值的增加而增强,当MOI值为50时,感染细胞进入S期和G_2期的细胞比率显著增加.结论:重组腺病毒rAd-hFGF-7可促进HaCat细胞的增殖,改变HaCat细胞周期.     

Antagonistic effect of 6-hydroxydopamine (6-OHDA) on generation of immunosuppressive protein of stress in the rat

Neonatal rats were injected with 6-hydroxydopamine (6-OHDA) for chemical sympathectomy. The rats were treated with restraint stress when they grew up to 56 d. It has-been found that the generation of immunosuppressive protein of stress (ISPS) is significantly reduced, suggesting that peripheral sympathetic nerve plays an important role in mediating the generation of ISPS.     

重组人碱性成纤维细胞生长因子诱导Balb/c3T3细胞增殖的实验研究

目的研究不同浓度重组人碱性成纤维细胞因子对Balb/c 3T3细胞增殖活性的影响.方法重组质粒pGEX-bFGF转入到DH5α大肠杆菌体内,增菌培养表达bFGF,亲和层析纯化,制备8种不同药物浓度的bFGF,分别进行诱导Balb/c 3T3细胞增殖实验研究,MTT法检测各组细胞增殖活性.结果 bFGF诱导Balb/c 3T3细胞增殖存在浓度依赖性,最适浓度为4～16μg/L.结论 bFGF具有较好的诱导Balb/c 3T3细胞增殖的能力,为后续研究工作奠定实验基础.     

整体煤气化固体氧化物燃料电池电热联产系统分析

整体煤气化固体氧化物燃料电池-燃气轮机混合发电系统(Integrated gasification solid fuel cell-gas turbine hybrid powergeneration system,IGFC-GT)是一种高效清洁合理的煤炭发电技术。混合系统的模型搭建及性能分析对于系统集成和商业运行有着重要的意义。通过查阅国内外在致力于提高SOFC发电效率方面的研究文献,并根据课题组的前期研究成果,基于商业软件Aspen Plus平台,搭建了IGFC-GT混合发电系统的电热联产系统模型图,分析了IGFC-GT电热联产系统的性能,结果表明该系统具有较高的发电效率及总能量利用率。     

重组人三叶因子3对细胞的增殖及迁移的影响

将重组人三叶因子3(Trefoil factor 3, TFF3)作用于人结肠肿瘤细胞,研究重组蛋白对细胞增殖的影响,结果发现该蛋白在较低的浓度(10～50 mg/L)下对细胞的增殖基本没有影响,在100～200 mg/L浓度下该蛋白对细胞仅有微弱的刺激作用,提高浓度对细胞增殖作用没有改变。同时研究了TFF3对损伤的单层结肠肿瘤细胞迁移的影响,发现TFF3对细胞有明显的促进迁移作用。     

光亮碱性Zn-Ni合金电镀工艺研究

采用碱性锌酸盐镀液为基础,加入适当的络合荆、镍盐和自制添加剂与光亮剂,研究成功了一种新的光亮碱性Zn—Ni合金电镀工艺,采用霍尔槽试验探讨了添加剂、光亮荆、主盐的影响,检测了镀液、镀层性能．结果表明：该镀液成分简单、操作方便,镀液分散能力和复盖能力好,所得锌镍合金镀层结晶细致、光亮平整,其耐蚀性明显优于锌镀层,适用于钢铁件高耐蚀性电镀．     

麝香百合组培快速繁殖技术研究

通过对百合球茎鳞片离体组织培养的研究,探讨了丛生芽诱导、继代增殖培养、生根培养及移栽等方面的组培快速繁殖以及生产上实用的组培技术,结果表明,MS 6-BA3.0mg\L NAA0.5mg\L KT0.14mg\L 2.4-D0.3mg\L 蔗糖35mg/L为适宜的诱导培养基,其诱导率为92%;MS 6-BA2.5mg/L NAA0.5mg/L KT0.1mg/L 2.4-D0.2mg/L 蔗糖65g/L为最佳的继代增殖培养基,其繁殖倍数为10.9倍,1/2MS NAA1.0mg/L 蔗糖35g/L的培养基诱导生根效果较好, 其生根率为86.7%,移栽成活率为100%,这为麝香百合大规模工厂化育苗提供了理论依据和技术保证。     

Qualitative relations of egg size,egg production and colony size in some ponerine ants (Hymenoptera: Formicidae)

The sizes of eggs and reproductives of 13 species of ponerine ant were measured, and information about the mature colony sizes, ovarial anatomy and individual fecundities of a selection of ponerine species was assembled. Inter-specific variation in the size of colonies was found to be poorly related to aspects of individual fecundity, and although the few cases of polygyny were associated with larger colony sizes, monogynous species often attained even larger sizes. Within the subfamily individual fecundity may be increased primarily by a decrease in the maternal investment in individual eggs, and perhaps to a lesser extent by an increase in the number of ovarioles in each ovary or an increase in the length of each ovariole. The diet of a species and the longevity of adult worker ants relative to the duration of their larval development are suggested as factors besides reproductive characteristics that might also contribute to differences in colony size.     

黑种草籽调节黑素合成的药效物质基础

以高效液相色谱( HPLC)法对黑种草籽不同体积分数的醇提取物进行色谱峰表征,以体外培养的小鼠B16黑素瘤细胞为模型,采用MTT法测定黑种草籽不同体积分数的醇提取物对B16黑素瘤细胞增殖的影响,采用L-DOPA氧化法测定黑种草籽不同体积分数的醇提取物对B16黑素瘤细胞酪氨酸活性的影响,采用NaOH裂解法测定黑种草籽不同...