三种药物对鸡人工感染柔嫩艾美耳球虫病的疗效比较

采用瘟毒杀星散、球痢清、地克珠利三种不同的抗球虫药物进行抗人工感染柔嫩艾美耳球虫的效力试验,结果表明:三种不同药物的高剂量组即300mg/kg饲料混饲,其抗球虫指数(ACI)均在185以上,差异不显著(P>0.05).但中剂量组即250mg/kg饲料混饲,球痢清和地克珠利组的死亡率分别比瘟毒杀星散组高4.1 5%和3.55%,差异显著(P<0.05),卵囊值差异亦显著(P<0.05),但抗球虫指数(ACI)、病变值差异不显著(P>0.05).瘟毒杀星散低剂量组与球痢清和地克珠利低剂量组相比,其相对增重率、抗球虫指数(ACI)和病变值差异不显著,(P>0.05),但卵囊值差异极显著(P<0.01).     

基于颜色特征的油茶叶片病斑分割研究

油茶叶片病斑分割是提高油茶病害图像识别准确率的前提。根据油茶炭疽病、软腐病和煤污病等主要病害的症状特征,提出了基于超绿特征的油茶叶片病斑检测方法。对比分析了HSI、YCbCr和Lab颜色模型的各个颜色分量以及超绿特征对油茶叶片病斑分割性能的影响。对于炭疽病和软腐病,超绿特征的单阈值分割性能最好,色度a的分割性能较好,色差Cb和色差Cr的分割性能稍差,色调H的分割性能最差,其Ⅰ型错误率很高。对于煤污病,亮度L的分割效果最好,亮度Y的分割效果次之。试验结果表明,超绿特征对于三种典型油茶病害图像的综合分割性能较高,具有较低的Ⅰ型错误率和Ⅱ型错误率,其平均检测精度达到81.69%以上,可以降低因叶柄和叶脉影响而产生的分割错误。     

基于蛋白质组学技术筛查维吾尔族妇女宫颈癌前病变患者血浆蛋白预警标志物

 依托蛋白质二维液相色谱和质谱技术,寻找与新疆维吾尔族妇女宫颈癌癌前病变高度宫颈鳞状上皮内瘤样变(HSIL)相关的血浆预警蛋白标志物,旨在对宫颈癌做到早期预防和诊断。收集维吾尔族HSIL(21例)和宫颈炎(22例)患者血浆标本并制备血浆低丰度蛋白质组样品,通过对蛋白质二维液相色谱系统的分离与鉴别分析,筛选出差异表达的组分,并运用质谱和生物信息学技术进行鉴定和功能识别分析。确定了宫颈炎和HSIL患者的基于蛋白质等电点和疏水性特征的血浆低丰度蛋白质组图谱,筛选出10个差异表达蛋白组分。结果表明,在HSIL患者血浆中3个低丰度蛋白组分含量明显上升,1个组分含量下降;而通过质谱技术鉴定出31种蛋白质,其中4种蛋白质与肿瘤进程密切相关,分别是代谢相关蛋白(APOA1)、信号转导相关蛋白(mTOR、EphA3)和免疫功能相关蛋白(HLA-DQB1)。本文针对维吾尔族妇女这一宫颈癌高发人群,展开癌前病变患者血浆低丰度蛋白组表达水平的研究,鉴定出多种差异表达血浆蛋白预警标志物,为本区宫颈癌的预防和早期鉴别诊断及癌变机制研究提供了依据。     

Pubertal changes in the medio-basal hypothalamic area after neonatal suprachiasmatic nucleus lesions in the rat

Summary In order to get more insight into the mechanism by which the onset of puberty is controlled, a developmental study on the displacement of catalase- and dopamine-containing cells in the hypothalamic region was done in rats which received a neonatal lesion of the suprachiasmatic nucleus. The displacement of cells is delayed after these lesions. However, the time lost at the beginning of the displacement is made up at the end of the migration.     

Antagonism by thyrotropin-releasing hormone (TRH) of pentobarbital-induced hypothermia in rats with brain lesions

Summary Anesthesia with a large dose of pentobarbital (55 mg/kg, i.p.) caused a sustained decrease in brain temperature (Tb), which was monitored with a probe placed in the midbrain reticular formation. The administration of TRH to the lateral ventricle antagonized this hypothermia. None of the acute surgeries examined in this paper (adrenal-demedullectomy, septal knife cuts, electrolytic lesions of the hypothalamus and midbrain knife cuts) had any essential effect on this antagonism by TRH. These results suggest that centrally-administered TRH exerts its effect on thermoregulation, at least in part, through brain structure(s) caudal to the midbrain.     

皮质脊髓束纤维终末的超微结构研究

采用顺行溃变法对猫皮质脊髓束在脊髓内投射纤维终末的超微结构和突触联系进行了研究．用电解损毁皮质２～５ｄ后，电镜下发现颈髓内皮质脊髓束纤维终末存在三种溃变形式：即电子致密型、神经微丝型和电子透明型，以电子致密型最为多见．溃变的纤维终末较粗大，主要含有大量密集的圆形突触小泡．主要形成不对称型的轴——树突触、轴——体突触、树——轴——树系列突触，并参与形成以树突为中心的突触复合体     

Effects of carotid sinus nerve stimulation on respiratory sinus arrhythmia and respiratory blood pressure waves of the dog

Summary The effect on the amplitudes of RSA and RBPW of the time of the stimulus in the cardiac cycle, and also of continuous stimulation were studied. When the stimulus train was applied near peak systole the amplitudes of RSA and RBPW decreased. Stimulation in late systole increased both RSA and RBPW. Continuous stimulation did not exert any effects on RSA and RBPW.     

膝侧副韧带损伤后本体感觉恢复的训练研究

目的探讨内外侧副韧带扭伤后膝关节本体感觉恢复的训练方法.方法将侧副韧带扭伤者21例分为膝关节本体感觉强化组(11例)、对照组(10例),本体感觉强化组应用本体感觉强化训练,对照组应用一般康复训练方法;实验前后膝关节位置觉测定.结果在再成角测试中,实验前两组位置觉偏差基本一致,伤膝平均偏差(4.32±2.17)°、健膝(2.70±1.53)°,伤膝大于健膝(P<0.05);伤膝各角度之间平均偏差无明显差异(P>0.05).实验后本体感觉强化组伤膝的平均偏差为(2.52±1.51)°,对照组为(3.01±1.51)°;两组之间平均偏差存在明显差异(P<0.01).结论本体感觉强化的康复训练使膝关节功能恢复,损伤组织愈合,位置觉偏差正常,再损伤率低.     

基于改进YOLOv5的胶囊内窥镜病灶区域检测

针对目前胶囊内窥镜病灶检测模型存在检测疾病单一且效率低等问题,提出了一种基于YOLOv5的胶囊内窥镜病灶区域检测方法。该方法在原始YOLOv5基础上进行了如下改进：首先,在主干网络Backbone部分,添加一个CBAM(convolutional block attention module)模块,增强模型对重要特征的突出能力;其次,在头部网络Head部分,添加一个检测头,增强模型对小目标的检测能力;最后,将原始YOLOv5的泛化交并比(generalized intersection over union, GIoU)损失函数替换成完整交并比(complete intersection over union, CIoU)损失函数,使模型训练时更快地收敛。本文提出的方法在长江大学第一临床医学院提供的胶囊内窥镜影像数据上进行了实验,精确率达到了93.6%,召回率达到了94.3%,mAP@0.5达到了97.2%,而且检测速度达到了每帧0.027 2 s。实验结果表明提出的方法是有效的、灵活的、鲁棒的,能够满足临床医学诊断的实际需求。     

Characterization and mapping of a lesion mimic mutant in rice （Oryza sativa L.）

A rice initiation-type lesion mimic mutant (lmi) was identified, which was isolated from an indica rice Zhongxian 3037 through γ radiation mutagenesis. Trypan blue staining and sterile culture revealed that the mutant spontaneously developed lesions on the leaves in a developmentally regulated and light-dependent manner. Genetic analysis indicated that the lesion mimic trait was controlled by a single resessive locus. Using public molecular markers and an F2 population derived from lmi and 93-11, we mapped the lmi locus to the short arm of chromosome 8, nearby the centromere, between two SSR markers RM547 and RM331. The genetic distance was 1.2 and 3.2 cM, respectively. Then according to the public rice genomic sequence between the two SSR markers, lmi was further finely tagged by three CAPS markers: C4135-8, C4135-9 and C4135-10. And lmi locus was a co-segregated with marker C4135-10, providing a starting point for lmi gene cloning.