EUT对TEM传输室特性阻抗影响的边界元分析

采用边界元法分析了金属箱体的受试设备对横电磁波传输室特性阻抗的影响．计算结果表明，当受试设备不满足“三分之一准则”时，对特性阻抗的影响达5Ω以上，当满足“三分之一准则”时，对特性阻抗的影响小于2Ω．测量结果证实了计算方法的有效性，所得结论对TEM传输室的设计与测试具有重要意义。     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system

The human adenovirus type 5 E1A, a tumor- suppressor gene[1], codes for two major related proteins of 243 amino acids (12S) and 289 amino acids (13S) by al-ternative splicing in two exons[2]. Studies have been shown that E1A can regulate expression of many genes and cell cycle[3]. Both in vitro and in vivo experiments indicated that E1A could induce tumor cells differentia-tion, convert tumor cells into an epithelial phenotype, in-hibit tumor cell growth and metastasis and strongly en-ha…     

Mechanical Studies on Treatment of Malignant Tumour by Ultralow Frequency Pulsed-Gradient Magnetic Fields

Ultralow frequency (ULF) pulsed-gradient magnetic field (with the maximum intensity of 0.6-2.0 T, gradient of 10-100 T·m-1, pulse width of 20-200 ms and frequency of 0. 16-1. 34 Hz) treatment of mice can inhibit murine malignant tumour growth and can induce apoptosis of cancer cell. The apoptotic cancer cell contracted, became rounder and divorced from adjacent cells; the heterochromatin condensed and coagulated together along the inner side of the nuclear membrane; the endoplasmic reticulums expanded and fused with the cellular membrane; many apoptotic bodies which were packed by the cellular membrane appeared and were devoured by some lymphocytes and plasma. By Lorentz force the magnetic field keeps the moving ions within bounds of Larmor radius. Thus, penetrating capability of the positive and negative ions through the cell membrane was affected, even the role on the cell membrane formed.     

从黄土Sr同位素探讨海水87Sr/86Sr变化的原因

目前多数认为河流输入是引起海水^87Sr／^86Sr变化的最主要因素，对此推论并没有提供确实的定量分析和依据．通过对黄土高原洛川剖面黄土—古土壤Sr同位素组成和沉积速率等的定量计算说明，在过去2．5Ma期间，海水^87Sr／^86Sr上升总量仅是河流输人海洋总量的大约1/10，而其9／10被海底玄武岩的热液蚀变输入所抵消．海底玄武岩的热液蚀变输入对海水^87Sr／^86Sr变化的总影响量应近似等于河水输入对海水^87Sr／^86Sr变化的总影响量．海水^87Sr／^86Sr变化的原因是河水输入与海底玄武岩的热液烛变输入联合作用的结果．     

光滑流形在(Z2)2作用下信息为{(2,2,0),(2,0,2),(0,2,2)}的上协边类

设(Z2)^2作用于光滑闭流形M^n，其不动点集的法丛的信息为P＝{(2，2，0)，(2，0，2)，(0，2，2)}，Jn^4，2(P)是有代表元M^n且具有上述性质的n维上协边类[M^n]构成的集合．作者通过构造上协边环M0n的一组生成元决定了Jn^4，2(P)的群结构．     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

尕斯油田N_2~1油藏南区储层非均质性研究

针对尕斯油田N21油藏南区储层非均质性严重的特点,从层间、层内及平面非均质性3个方面进行了系统研究.层间非均质性相对较弱,层间隔层分布较为稳定,各油组砂层发育情况差别不大.层内非均质性较强,并且与沉积微相密切相关.单砂层垂向上渗透率的变化以正韵律和复合韵律模式为主,局部发育反韵律模式.按照非均质性的强弱,沉积微相依次为:分流河道、河口砂坝、分流间湾、远砂坝.统计了平面上不同微相砂体的几何形状,并且采用流动单元法综合评价储层的平面展布特征.     

彗星电泳检测细胞DNA损伤应用新进展

彗星电泳是近年发展起来的一种测定单个细胞DNA损伤的方法 ,因其操作简单、快速及灵敏等特点 ,应用范围十分广泛 .着重介绍了这一技术的发展、图形分析及应用进展     

熔融碳酸盐燃料电池阴极材料制备与导电性

研究了熔融碳酸盐燃料电池（MCFC）阴极材料锂铁氧化物电极的导电性能。实验结果表明在合成锂铁氧化物的过程中，使Li2CO3轻微过量，可以提高锂铁氧化物电极的导电性。在同一温度下，随着Li:Fe值的增大，锂铁氧化物电极的导电性增加。相同Li:Fe条件下，锂铁氧化物电极的导电性随着温度升高按指数规律增加。扫描电镜研究（SEM）表明，Li:Fe比值不同，制备的锂铁氧化物电极表面形貌也不同。