CD-ROM的支持标准及其驱动器速度分析

介绍了作为多媒体计算机重要部分ＣＤＲＯＭ的各种国际标准，同时以１２Ｘ驱动器和１６Ｘ驱动器为例对ＣＤＲＯＭ驱动器的读写速度进行了分析，并对用户选购ＣＤＲＯＭ提出了要注意的事项     

SciFinder Scholar数据库的采购研究

通过对CA光盘数据库和网络版数据库内容、功能、后处理及价格等的比较,全面分析了网络版数据库的优点,结合试用、培训结果以及其他高校的经验,最终确定了CA网络版数据库采购方案。     

DVD-Audio DVDPlus及其与SACD之比较

随着环绕声系统在家庭中的普及,以及DVD的发展,越来越多的消费者希望听到多声道的音乐节目.DVD-Video提供环绕声,采用的是Dolby Digital和DTS编码的音频.DVD-Audio和SA-CD提供的是不经压缩的高质量的环绕声音频,而只有DVD-Audio提供额外的视频、静止画面和其它非音频的功能.DVD-Audio和SA-CD的同时存在给消费者带来了困惑和麻烦.但DVDPlus盘的到来可能会对DVD-Audio在音乐市场的竞争中有所帮助.本文阐述了DVD-Audio,DVDPlus盘的特点、采用的技术以及他们与SACD的不同.     

高校图书馆随书光盘管理模式的演变

在介绍高校图书馆传统的人工借阅模式、网络化管理模式和社会化光盘管理模式的基础上,指出了网络化管理模式是目前高校图书馆的主流模式,未来高校图书馆将有可能实行社会化管理模式。     

Anti-CD44 mAb remodels biological behaviors of spheroid cells with stemness from human ovarian cancer cell line SKOV-3

There is accumulating evidence that cancer stem cells (CSCs) play an important role in tumor progression. Novel strategies targeting CSCs have been widely researched. In the present study, we explored whether such CSCs existed in human ovarian cancer (OVCA) cell line and whether anti-CD44 antibody had effects on such subpopulation. We isolated and identified spheroid cells from SKOV-3. Then we used A3D8, an anti-CD44 mAb to treat spheroid cells with so-called "stemness". Effects of A3D8 on spheroid cells’ biological behaviors were examined. Our findings showed that there was a small subpopulation that had so-called "stemness" in SKOV-3 cell line. Against spheroid cells, A3D8 can (1) inhibit cell proliferation; (2) change cell cycle distribution and expression of p21, CDK2 and cyclinA; (3) enhance cisplatin (DDP)-induced apoptosis; (4) promote cell differentiation; (5) inhibit clone formation efficiency; (6) reduce invasive efficacy; (7) inhibit tumorigenicity. Thus, to sum up points which we have just showed, spheroid cells isolated from SKOV-3 can be used as an appropriate in vitro model for relevant study of human ovarian CSCs. And our results reasoned that anti-CD44 therapy may become a potential promising strategy for OVCA treatment.     

《中国学术期刊(光盘版)》评析

就《中国学术期刊 (光盘版 )》的内容、特点、检索功能及其对图书馆产生的影响和效益等问题进行了介绍和评析。     

Two tyrosines in CD3s-ITAM are required to induce T lymphocyte apoptosis

CD3ε of T cell antigen receptor complex (TCR/CD3) plays an important role in the resembling of the complex and activation signaling through its conservative immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic tall. Previous study showed that a chimera molecule, consisting of the extracellular-transmembrane domain of human CD8α fused to the cytoplasmic domain of CD3ε, induced apoptosis of T lymphocytes, indicating that apoptotic signals were transduced through the CD3ε-ITAM. To elineate involvement of the two tyrosines in apoptotic signaling pathway, cDNAs with mutations at Y170F, Y181F and Y170F/Y181F in CD8ε-ITAM were made by point mutation and PCR, and then cloned into pcDNA3 eukaryotic expression vectors. Stable expression cell lines were established after transfection of the expression vectors into CD8^- Jurkat T lymphocytes. Stimulation of these cell lines with anti-CD8 monoclonal antibody showed that only the cells with expression of wild type chimera CD8-ε died by apoptosis, but not those cells with expressions of mutated CD8-ε chimera, indicating that the two tyrosines in CD3ε-ITAM were required for the apoptotic signal transduction in T lymphocytes.     

Circular dichroism spectra of different structures formed by the oligonucleotides

Under different conditions, oligonucleotides can form several different DNA structures such as duplex, triplex and quadruplex. All these structures exhibit an obvious difference in their CD spectra. The common characteristic is that they show a negative band at 240 nm, while in the range of 260–300 nm, the spectra are different from each other. Many factors such as chain direction, sugar puckering mode, orientation of the glycosyl bond, base stacking and sequence can affect their conformation and then show diversity and complexity in their spectra. By studying and comparing these spectra, more information about their conformations can be obtained to help predict some new structures. Furthermore, the spectra can also provide a base to study their potential biological functions.