随书光盘(磁盘)的著录与管理

简单介绍了随书光盘的特点,阐述了随书光盘的著录方法与管理办法。     

Prolactin inducible protein in cancer, fertility and immunoregulation: structure, function and its clinical implications

Prolactin inducible protein (PIP) is a 17- kDa single polypeptide chain, known by various names due to its versatile nature and function in human reproductive and immunological systems. It is expressed in several exocrine tissues such as the lacrimal, salivary, and sweat glands. Its expression is up regulated by prolactin and androgens, and estrogens down regulate it. Due to its over-expression in metastatic breast and prostate cancer, presently PIP is considered as a prognostic biomarker. Moreover, its aspartyl-proteinase nature suggests its role in tumor progression. PIP has unique features because it is small in size and plays multiple important functions. Its ability to bind potentially with CD4-T cell receptor, immunoglobulin G (IgG), actin, zinc α2-glycoprotein (ZAG), fibronectin and enamel pellicle, reveals its important biological functions. This is the first comprehensive review on the structure and functional analysis of PIP and its clinical applications. Received 04 August 2008; received after revision 09 September 2008; accepted 15 September 2008     

hShroom1 links a membrane bound protein to the actin cytoskeleton

hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domain, ASD2 (Apx/Shroom domain 2). mRNA analysis suggests that hShrm1 is expressed in brain, heart, skeletal muscle, colon, small intestine, kidney, placenta and lung tissue, as well a variety of melanoma and other cell lines. Co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) experiments indicate that hShrm1 and MCAM interact in vivo and by immunofluorescence microscopy some co-localization of these proteins is observed. hShrm1 partly co-localises with β-actin and is found in the Triton X-100 insoluble fraction of melanoma cell extracts. We propose that hShrm1 is involved in linking MCAM to the cytoskeleton. D. E. Dye, S. Karlen: These authors contributed equally to this work. Received 09 October 2008; received after revision 23 November 2008; accepted 09 December 2008     

无血清分离人结直肠癌细胞的体外培养形态及标志物表达特征

目的探讨人结直肠肿瘤干细胞在体外分化过程中细胞形态和干细胞相关标志物CD133的表达变化,为进一步研究结直肠肿瘤干细胞分化走向提供实验依据。方法取来源于人结直肠癌的细胞系HCT116,无血清培养分离出CD133+细胞,加血清诱导分化,相差显微镜下观察其形态变化;在未分化状态下无血清培养第7天和14天与血清诱导分化后收集细胞,利用流式细胞仪检测干细胞标志物CD133的表达量,采用激光共聚焦检测CD133表面标记分子的表达。结果 1)细胞形态:无血清培养分离的CD133+细胞,在生长过程中聚集成规则的细胞球,血清诱导后即贴壁生长,贴壁形态与同来源细胞形态一致,且再次无血清悬浮培养后聚集成球稳定生长。2)标志物变化:结直肠肿瘤干细胞未分化时CD133表面标记分子高表达,流式细胞仪检测未分化细胞CD133第7天表达率为(20.4±0.52)%,第14天表达率为(78.5±2.80)%,分化后表达率为(0.50±0.17)%。结论细胞形态和标志物表达改变均表明高表达CD133+的HCT116结直肠癌肿瘤干细胞可定向分化为同源的结直肠癌细胞,CD133+细胞经血清诱导后表达下调而使细胞失去干细胞特性。     

Hyaluronan

The polysaccharide hyaluronan is an essential component of the vertebrate extracellular matrix and also produced by viruses, bacteria and fungi. Although the hyaluronan polymer is simply a disaccharide that repeats many thousands of times, it has an amazing array of biological functions and medical uses. For example, it is an efficient space filler that maintains hydration, serves as a substrate for assembly of proteoglycans and cellular locomotion, regulates cellular function and development, and is involved in tumor progression, inflammation and wound healing. Its physical properties and biocompatibility also make it of considerable importance in the development of engineered tissue, biomaterials and in clinical applications. Received 23 January 2007; received after revision 25 February 2007; accepted 22 March 2007     

小鼠胸腺髓质型CD8单阳性细胞两个亚群功能比较

ＴＣＲαβ＾＋ＣＤ４＾－ＣＤ８＾＋细胞为异质性细胞群体,可能还要经历表型乃至功能的进一步成熟。利用细胞表型配伍分析,将髓质型ＣＤ８ＳＰ细胞分为７个亚群,并推测其表型成熟的前后顺序。为了进一步印证发育过程,分离纯化６Ｃ１０＾－ＣＤ６９＾＋和６Ｃ１０＾－Ｑａ－２＾＋ＣＤ８ＳＰ细胞亚群,按其表型,分别代表髓质ＣＤ８ＳＰ细胞发育成熟过程的早中期和最后阶段,比较它们之间是否存在功能差异,并且以脾脏ＣＤ８＾Ｔ     

2017—2019 年 CD ( SD)大鼠 240 个 SNP 位点遗传检测结果分析

摘要:目的 2017—2019 年北京维通利华实验动物技术有限公司(以下简称,维通利华) 连续三年参与美国 CharlesRiver Laboratories( CRL)对全球各地设施 CD( SD)大鼠生产群的遗传检测,以评估种群的遗传稳定性、杂交系数及各设施间种群的差异。 方法 从大鼠耳部样品中提取 DNA,使用荧光标记法检测 240 个单核苷酸多态性( SNP ) 基因位点,通过 GenA1Ex6. 5 软件统计分析得到位点多态性( P) 、观测杂合度( Ho ) 、期望杂合度( HE ) 、近交系数( F)和遗传分化系数( FST )等。 结果 位点多态性比例分别为 73. 6% ,76. 9% 和 74. 2% ,遗传具有多样性且保持稳定;杂交系数分别为-0. 02、-0. 06 和-0. 07,符合随机交配特性;遗传分化系数( FST ) 在 0. 05 上下波动。 结论 维通利华的 CD( SD)大鼠种群保持稳定的遗传多样性,符合随机交配方式繁殖,与 CRL 美国基础种群呈低至中等程度的遗传分化,定期的引种措施为保持种群的杂交性和稳定性发挥重要作用。     

基于DSPN的CSMA/CD的建模和性能分析

近年来，以太网在自动化行业得到了广泛应用，对其MAC层采用的CSMA／CD协议进行性能评价已成为必须。本文基于确定与随机Petri网(DSPN)对CSMA／CD进行了模型抽象，并且利用软件DSPNexpress-NG对该模型进行性能分析，所得结论对以太网组网有较好的参考价值。     

毒死蜱降解菌CD7的筛选与植物根际促生菌JD37的联合应用

筛得一株毒死蜱降解菌CD7,联合植物根际促生菌JD37,研制了一种能修复土壤的农药污染,同时促进植物生长的复合型土壤改良剂.实验结果显示:将CD7和JD37发酵液按体积比1∶1使用,可以促进植物生长,并在25 d内降解土壤中约66.43%的毒死蜱.在载体量相同情况下,蚯蚓粪比滑石粉吸附更多混合菌株.在室温贮藏一个月,活菌数为4.81×10~7CFU/g;制备的复合改良剂与土壤按质量比1∶1使用时,能促进植物生长、提升土壤酶活性、增加土壤中微生物的数量.     

图书馆附书光盘的管理和利用研究

如何科学管理和有效利用附书光盘已成为目前高校图书馆的重要工作内容之一。文章概述了附书光盘的几种管理模式,重点介绍了基于博文非书资料管理系统的附书光盘网络化管理模式,提出了附书光盘管理中的关键问题及提高利用率的重要方法。