基于非随机数据抽样的Matlab模型对艾滋病疗法的评价及预测

对所给医学数据进行分析,建立数据与服药周次的函数关系,同时用Matlab建立模型对艾滋病的四种疗法进行分析,在分别考虑CD4浓度、HIV浓度、治疗费用的条件下,利用曲线拟舍、最小二乘法等方法评价四种疗法或预测继续治疗的效果.     

Saccharide Recognition by Oligo( meta-ethynylpyridine)s in Protic Media

1Introduction Previously, we have reported tris(ethynylpyridine) macrocycles as synthetic receptors for ribofuranosides, deoxyribofuranosides, and glucopyranosides[1]. The hydrogen-bonding skeletons of the macrocycles consisted of "meta"-tethered ethynylpyridine trimers. If the ethynylpyridine unit is polymerized, the resulting oligo- and poly(meta-ethynylpyridine) s would adopt unfolded, somewhat zigzag conformations because each pyridine nitrogen is mainly located on opposite sides of the ethynediyl bonds to cancel the dipoles. When the polymer meets with a saccharide, its pseudolinear conformation may be guided to a well-ordered helical structure in order that the nitrogen atoms of the pyridine rings inwardly interact with peripheral saccharide-OH groups in a manner similar to those of the macrocycles (Fig. 1). Thus, the chirality of saccharides added can be transferred to the helical sense of the polymer, depending on the stereochemistry of the saccharides.     

壳寡糖对体外诱导的非酒精性脂肪肝细胞模型的降脂机理研究

为探讨壳寡糖在体外对肝脂质积累的抑制效果及潜在作用机制,选用油酸钠诱导HepG2细胞建立非酒精性脂肪肝细胞模型,通过油红O染色及各试剂盒检测胞内总脂质积累、甘油三酯、总胆固醇、高低密度脂蛋白胆固醇及游离脂肪酸的含量,实时定量PCR及western blot检测细胞内脂代谢相关基因和蛋白的表达。结果发现,4.0mg/mL的壳寡糖对肝脂质蓄积具有明显的抑制作用,并降低了二酰基甘油酰基转移酶2(DGAT2)、肝X受体α(LXRα)、过氧化物酶体增殖物激活受体-γ(PPARγ)、孕烯醇酮X受体(PXR)和分化簇36(CD36)的mRNA水平和蛋白水平的表达。研究结果表明,壳寡糖可以通过调节脂肪酸吸收和甘油三酯合成通路从而降低非酒精性脂肪肝细胞模型中的脂质积累。希望本研究能够为利用壳寡糖开发具有降脂护肝功能的膳食补充剂提供理论参考。     

CD23 (the low-affinity IgE receptor) as a C-type lectin: a multidomain and multifunctional molecule

This review, regards the low-affinity receptor CD23 as a C-type lectin and compares it with other C-type lectins and C-type lectin-like receptors. C-type lectins such as the asialoglycoprotein receptor, as well as the dendritic cell immunoreceptor and the dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin on dendritic cell lectin, possess amino acid sequences which interact with Ca⁺⁺ and sugar, and many of them possess an endocytosis signal sequence that includes tyrosine or serine in the cytoplasmic region. In contrast, natural killer receptors lack the Ca⁺⁺ and sugar-binding amino acids but conserve homologous cysteines in the form of C-type lectin, and possess an immunoreceptor tyrosine-based inhibitory motif in the cytoplasmic region which inhibits killer activity when they recognize the self major histocompatibility (MHC) class I molecule. Since human CD23a form has a similar amino acid sequence, the possibility that this sequence is an endocytosis signal or an ITIM is discussed. The function of the reverse RGD and RGD-binding inhibitory peptide in human CD23 from the point of view of the relation between a C-type lectin and MHC class II molecules is also considered. Received 21 May 2001; received after revision 28 November 2001; accepted 29 November 2001     

FK506对人外周血T淋巴细胞CD_(28)分子表达的体外抑制作用

应用淋巴细胞体外活化模型和流式细胞分析技术，观察异体角膜体外对人外周血Ｔ淋巴细胞ＣＤ２８分子表达的调节和ＦＫ５０６对Ｔ淋巴细胞ＣＤ２８表达的抑制作用．结果显示：空白对照组Ｔ淋巴细胞ＣＤ２８表达为２１．３％；受异体角膜或ＰＤＢ刺激后，Ｔ淋巴细胞ＣＤ２８表达分别为５２．４％和８１．４％，同时加入ＦＫ５０６的实验组，Ｔ淋巴细胞ＣＤ２８表达分别为２４．５％和４１．９％·结果表明异体角膜体外能够刺激Ｔ淋巴细胞ＣＤ２８分子表达，ＦＫ５０６体外能够明显抑制Ｔ淋巴细胞ＣＤ２８分子表达和活化．     

Expression of LIF,VEGF, CD57 and CD68 after the transfer of rat embryos to mouse uteri

The high failure rate of interspecific preganacy is a major obstacle to the successful interspectific cloning of mammals,To in vestigate the reasons for the failure of inter-specfic pregnancy between rats and mice,we transferred rat blastocysts into mouse uteri on the third day of pseudopreg -nancy (D3),oure previous study showed that intact rat embryos could still be observed in mouse uteri on D9.In the present study ,we found that expression of CD57 and CD68 increased significantly at the maternal -fetal interface fol-lowing the transfer of rat embryos,Similarly ,Leukaemia inhibitory factor(LIF) expression increased ,but vascular endothelial growth factor(VEGF) expession decreased,In a co-culture system ,the percentage of rat ectoplacental cones (EPCs) with adhesion and outgrowth and outgrowth area on mouse uterine decidual cells were less than that of mouse EPCs,These results indicate that an increase in the immunological rejection response and a decrease in the in vasiveness of rat embryos may be important reasons for the failure of interspecific pregnancy between rat and mouse.     

Protection of phosphatidyl-choline to photosystem II membrane during heat treatment

Photosystem II membrane was reconstituted with phosphatidylcholine (PC) with different kinds of fatty acyl chains and the protection of PC to photosystem II (PS II) membrane during heat treatment was investigated using oxygen electrode, variable fluorescence and circular dichro-ism (CD) spectroscopy. Heat treatment decreased the oxygen evolution rate and the F'v/Fm' ratio of PS II membrane and influenced CD spectra of PS II membrane, but PC inhibited the effect of heat treatment on the oxygen evolution rate, the F'v/F'm ratio and CD spectra of PS II membrane. The results indicate that PC can protect PS II membrane against heat treatment and the alterations in the unsaturated fatty acid extent in PC can cause the changes of the protection ability.     

新型数字式多路温度巡检仪的设计

设计了一个可对任意一路进行定点温度数字显示的多路温度巡检仪,其包含:温度信号检测及放大部分、数字控制部分、A/D转换部分及数字显示四大部分。该系统的工作原理为:AD590传感器将温度信号转化成电流信号,再经放大电路变换成与温度成线性关系的电压信号,再由数字控制电路送到A/D转换器,最后通过数字显示器显示出测量的温度。其测量范围为一55℃～150℃,具有实用性强、可靠性高、测量精度高等特点。     

CD-1600血球计数仪溶血剂的研制和应用

研制出适合于美国CD－1600型全自动血球计数仪溶血剂，选择出最佳季铵盐－进口氯化十二烷基三甲铵，其最适浓度为35g/L .Hb测定以HiCN方法为标准，用自配溶血剂和进口溶血剂分别对98例临床全血标本进行对照测定，结果表明其WBC计数、WBC分类及其直方图高度相关，具有显著的相关性（P＜0．01，r＞0．8），两年多临床应用表明，自配溶血 剂性能稳定，配制简便，适合于大多数医院自行配制。     

新型嵌合补体抑制分子HCI-3的设计构建及真核细胞表达的研究

目的：基于抑制补体活化的两个关键环节，即C3／C5转化酶及MAC的形成，设计、构建及制备一种新型、高效的补体抑制剂．方法：首先设计引物，通过PCR技术扩增重组可溶性CD46／CD55／CD59嵌合分子cDNA片段，重组于pcDNA3．1( )真核表达载体上，构建兼有三分子功能的CD46／CD55／CD59嵌合补体抑制分子，命名为HCI-3(Human Chimeric Complement Inhibitor,HCI-3)．分别利用COS-7细胞和CHO细胞进行表达，并用抗人CDl6多抗及抗人CD55，CD59单抗对表达产物进行Western Blotting鉴定．结果：DNA测序结果证实，前端带有CD59信号肽序列，后端融合有编码6个组氨酸碱基的HCl-3 cDNA片段的阅读框完整．免疫印迹结果显示，表达的重组蛋白分别能与抗人CD46多克隆抗体和抗人CD55，CD59单抗结合．结论：成功构建并在真核细胞内表达了重组可溶性HCl-3分子，为进一步研制和开发新一代多功能、多靶点补体抑制剂奠定了基础．