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31.
Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression
vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine
deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence
analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide
by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank. 相似文献
32.
SrGa2S4: Ce blue TFEL device with a novel structure was prepared by electron beam evaporation. The dielectric constant of insulator
layer in this structure is far less than that in the conventional structure, so the hot-electrons can be accelerated in SiO2 layers. The stable TFEL with good chromaticity was achieved and the basic characteristics were investigated. 相似文献
33.
《科学通报(英文版)》1998,43(17):1480-1480
CD3ε of T cell antigen receptor complex (TCR/CD3) plays an important role in the resembling of the complex and activation signaling through its conservative immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic tail. Previous study showed that a chimera molecule, consisting of the extracellular-transmembrane domain of human CD8α fused to the cytoplasmic domain of CD3ε, induced apoptosis of T lymphocytes, indicating that apoptotic signals were transduced through the CD3ε- ITAM. To elineate involvement of the two tyrosines in apoptotic signaling pathway, cDNAs with mutations at Y170F, Y181F and Y170F/Y181F in CD8-ε-ITAM were made by point mutation and PCR, and then cloned into pcDNA3 eukaryotic expression vectors. Stable expression cell lines were established after transfection of the expression vectors into CD8+- Jurkat T lymphocytes. Stimulation of these cell lines with anti-CD8 monoclonal antibody showed that only the cells with expression of wild type chimera CD8-ε died by apoptosis, but not those cells with expressions of mutated CD8-ε chimera, indicating that the two tyrosines in CD3ε-ITAM were required for the apoptotic signal transduction in T lymphocytes. 相似文献
34.
It was found that C60Br24 transforms to C60 spontaneously in some solvents. The intermediate, C60Br8, can be separated in some cases. When the bromine trap, phenol, was added,p-bromophenol and other brominated product were obtained and identified. The influences of time, the kind of solvent, etc were
investigated. Possible mechanism was suggested.
Supported by the National Natural Science Foundation of China
Liu Ying: born in 1969, Ph. D 相似文献
35.
Search for new antiphytovirucides 总被引:3,自引:0,他引:3
The lead compound of substituted 2-(4′-hydroxyphenyl)benzimidazoles were modified by phosphorylation sulfonation and carboxymethylation.
The benzimidazoloamidophosphate (6) (7) have better activity against tobacco mosaic virus (TMV). The benzimidazolophenoxyacetic
acid, phenoxysulfonic acid containing trifluoromethyl, and nitro group have 77%≈85% efficiency for wheat rust disease.
Supported by the National Natural Science Foundation of China and National Laboratory of Elemento Organic Chemistry Nankai
University
Qu Fanqi: born in 1952, Associate professor 相似文献
36.
以太网建设中的关键技术问题 总被引:1,自引:0,他引:1
以太网拥有最大广泛的用户,以太网新技术的不断出现又给传统以太网注入了活力。深入理解以太网技术,对改造原有以太网和构造新网都有重要意义,本文论述以太网建设中的重要技术问题及建网实务。 相似文献
37.
再生丝心蛋白和羧甲基纤维素复合膜的特性及其在有机相葡萄糖生物传感器中的应用 总被引:1,自引:0,他引:1
用再生丝心蛋白和羧甲基纤维素构成的复合膜作为固定葡萄糖氧化酶的基质,并用红外光谱测试了该复合膜的结构,用扫描电子显微镜观察了其形态.用上述复合膜材料固定葡萄糖氧化酶和羧酸二茂铁(或7,7,8,8-四氰代二甲基苯醌,TCNQ)制成的葡萄糖生物传感器具有较好的灵敏度与稳定性 相似文献
38.
4种植物生长物质及温度胁迫对绿豆黄化幼苗肌醇磷脂代谢的影响 总被引:1,自引:1,他引:0
利用放射性标记和薄层层析(TIC)分离发现2,4-D,GA3和6-BA可不同程度地促进肌醇-1,4,5-三磷酸(IP3)水平的增加.在最适浓度下的作用效果是:GA3>2,4-D>6-BA.但脱落酸(ABA)没有引起IP3含量的变化.低温(0℃)和高温(35℃)胁迫亦引起IP3含量的增加.2,4-D,GA3,6-BA和温度胁迫的共同处理对IP3含量的增加有协同作用.表明2,4-D,GA3,6-BA和温度胁迫促进绿豆黄化幼苗肌醇磷脂代谢,ABA也许不参与绿豆黄化幼苗肌醇磷脂代谢. 相似文献
39.
利用手性源(2R,3S)-3,4-二甲基-2-苯基-1,4-氧氮杂-5,7-二酮(1)合成了具有光学活性的7,8-二甲基-3,6-二苯基-5-氧-8-氮螺[2.6]壬-4,9-二酮(3).测定了化合物(3)的晶体结构,并讨论了在环加成反应中的不对称诱导作用的机理. 相似文献
40.
前驱体溶液pH值对溶胶凝胶制备钛酸铋薄膜的影响 总被引:1,自引:0,他引:1
采用溶胶凝胶技术在石英玻璃基片上制备钛酸铋薄膜,讨论了前驱体溶液的酸碱度(pH值)对薄膜结构和结晶性的影响.pH<2和pH>35的前驱体溶液所制备的薄膜在晶化过程中出现Bi2Ti2O7和Bi2Ti4O11杂相.pH=25~35的前驱体溶液制备的薄膜是钙钛矿Bi4Ti3O12结构的取向薄膜 相似文献