武夷山南方铁杉针阔混交林不同地形土壤营养元素分析

南方铁杉（Tsuga chinensis var. tchekiangensis）在江西武夷山国家级自然保护区有较大的天然种群分布。探究江西武夷山南方铁杉针阔混交林中不同地形条件下土壤营养元素含量的特征,揭示地形因素对该地土壤营养元素迁移分布的影响,为深入研究土壤-植被相互关系以及生物多样性保护提供参考。     

微波消解一电感耦合等离子体质谱法测定龙牙百合花中的矿质元素

采用微波消解技术,建立了一种测定龙牙百合花中Mg、K、Ca、V、Mn、Fe、Co、Ni、Cu和zn等10种微量矿质元素的ICP—MS法．研究表明,该方法简便、快速、灵敏,标准曲线的线性都较好,适合于龙牙百合微量矿质元素的测定．试样分析结果显示,龙牙百合花中含有丰富的矿质元素,特别是Ca、Fe与zn．该方法为龙牙百合的利用提供有力的科学依据．     

碳排放、能源消费与经济增长的实证研究

从经济增长与资源环境的关系入手,构建经济增长、能源消费和碳排放的向量自回归模型（VAR）,运用脉冲响应函数研究三个变量之间的相互关系,并分别建立能源消费和二氧化碳排放为自变量的协整方程,结果表明: 经济增长与能源消费和碳排放具有长期的协整关系,经济增长每提高1%,能源消费增长增加0.240%,同时导致二氧化碳排放增加0.295%。这说明我国的高速经济增长过程中,付出了较大的资源环境自然代价。     

Ubi1 intron-mediated enhancement of the expression of Bt cry1Ah gene in transgenic maize (Zea mays L.)

The cry1Ah gene was one of novel insecticidal genes cloned from Bacillus thuringiensis isolate BT8. Two plant expression vectors containing cry1Ah gene were constructed. The first intron of maize ubiqutin1 gene was inserted between the maize Ubiquitin promoter and cry1Ah gene in one of the plant expressing vectors (pUUOAH). The two vectors were introduced into maize immature embryonic calli by microprojectile bombardment, and the reproductively plants were acquired. PCR and Southern blot analysis showed that foreign genes had been integrated into maize genome and inherited to the next generation stably. The ELISA assay to T1 and T2 generation plants showed that the expression of Cry1Ah protein in the construct containing the ubi1 intron (pUUOAH) was 20% higher than that of the intronless construct (pUOAH). Bioassay results showed that the transgenic maize harboring cry1Ah gene had high resistance to the Asian corn borers and the insecticidal activity of the transgenic maize containing the ubi1 intron was higher than that of the intronless construct. These results indicated that the maize ubi1 intron can enhance the expression of the Bt cry1Ah gene in transgenic maize efficiently     

大孔吸附树脂分离纯化唐松草总皂苷的工艺研究

选择5 种大孔吸附树脂(LSA20、21、30、40和 Diaon HP-20) 分离提取唐松草(Thalictrum simplex var. brevipes Hara) 中的总皂苷,考察大孔吸附树脂对唐松草总皂苷的吸附能力.结果表明Diaon HP-20的吸附效果最好,静态吸附容量达163.2×10-3 g/mL,动态吸附容量达102.5×10-3 g/mL.     

AiiA融合蛋白包涵体变性和复性的研究

通过IPTG诱导含有pGEXaiiA-B15质粒的工程菌使其大量表达AiiA融合蛋白.由于所表达的融合蛋白多为不可溶的包涵体,须经分离、变性溶解,再经过一个合适的复性过程才能实现变性蛋白的正确折叠,得到具有生物活性的蛋白.通过含N-十二烷基肌氨酸钠的缓冲液A溶液及尿素等变性剂使包涵体溶解,磷酸盐缓冲液透析复性.SDS-PAGE电泳表明包涵体已由不可溶转化成可溶的蛋白.抑菌试验证明复性后的AiiA蛋白对胡萝卜欧文氏软腐病菌引起的马铃薯软腐病有较明显的抑制作用.