融合模糊连通图和区域生长的MRI脑组织图像分割算法

针对磁共振脑组织图像中存在灰度不均匀,不利于分割的问题,提出了一种应用模糊连通图和区域生长的MRI(Magnetic Resonance Imaging)脑组织图像分割算法。首先用大津法对脑组织进行粗分割,得到脑白质部分的大致轮廓,。然后计算粗分割结构的中心点,根据中心点得到图像的模糊连通图。最后用区域生长算法对模糊连通图中的脑白质进行更精确的分割。试验结果表明,此方法能够精确的得到脑白质轮廓的边缘,并且大大降低了区域生长种子点位置和阈值选取对分割结果的影响。     

从系统科学角度探究“哲学始于惊讶”的本质

从柏拉图、亚里士多德开始就认为哲学始于惊讶,但对此问题的研究仍比较粗浅。惊讶是广泛的,它是获得智慧的人脑机能基本过程。惊讶的本质是人脑内思维非简单和的涌现。从系统科学角度探究哲学始于惊讶的丰富本质,使哲学作为智慧和一般知识的关系有一个新角度的认识。     

Immunorecognition of estrogen and androgen receptors in the brain and thoracic ganglion mass of mud crab, Scylla paramamosain

The brain and the thoracic ganglion of a crustacean can synthesize and secrete gonad-stimulating hormone (GSH) which stimulates the maturation of gonad. In the previous experiments, sex steroid hormones (estradiol, testosterone, progesterone, etc.) have been detected from the crustacean. However, the feedback regulation of sex steroid hormones on the brain and the thoracic ganglion of the crustacean has not been reported so far. In the present experiment, monoclonal antibodies were applied to investigate the immunorecognition of estrogen receptor (ER) and androgen receptor (AR) in the brain and the thoracic ganglion mass of Scylla paramamosain. The results showed that the distribution of the immunopositive substances of ER and AR was extremely similar. They distributed in the protocerebrum, deutocerebrum and tritocerebrum of the brain, and mainly in protocerebrum. In the thoracic ganglion mass, immunopositive substances distributed in the subesophageal ganglion, thoracic ganglion and abdominal ganglion, and mostly in subesophageal ganglion. Immunopositive substances of ER and AR mostly existed in the cytoplasm of neurons. The present study will provide morphological evidence for the origin and the evolution of ER and AR. In addition, the immunoreactivities of ER and AR suggested that the estrogen and androgen may be involved in the feedback regulation of crustacean neuroendocrine.     

晚期胃癌中的肿瘤异质性表达与侵袭转移的相关性研究

应用免疫组化和突变分析方法,分析晚期胃癌原发灶和转移灶nm23,S100A4和p53的表达差异,探讨肿瘤内部异质性在胃癌转移发生中的意义。结果发现转移抑制基因nm23在转移灶中的表达显著上调,转移促进基因S100A4在转移灶中的表达显著下调,癌基因p53在两者中的表达和突变没有明显差异。nm23在原发瘤中的表达下调以及S100A4和p53的过度表达与胃癌的高侵袭性显著相关。数据显示:nm23和S100A4在胃癌的转移中发挥作用,而p53是胃癌发生的一个晚期事件。     

PCR—SSCP研究肝癌原发灶和转移灶中P16^INK4exon2的突变

用两步ＰＣＲ法，成功地从石蜡包埋组织ＤＮＡ中扩增了Ｐ＾１６ＩＮＸＫ４ｅｘｏｎ２片段；并用单链构象多态分析ＰＣＲ产物的差异。     

快速自顶向下优化神经网络结构的方法

引荐了一种优化神经网络结构的改进方法。近年来，各种神经网络结构优化方法相继被提出，但素负盛名的还是神经中枢手术优化算法(OBS)。提出一种与修剪技术相关的快速方法，这种方法起始于适当复杂的初始网络，然后剔除冗余的神经元。研究说明，这种快速方法与以往的常规神经中枢手术优化算法相比，具有更好的归纳性能、更简单的网络结构和更快的学习速度。     

狗脑抗吗啡镇痛肽“60a”的分离纯化及部分一级结构

本实验室从正常狗脑中又分离获得一个暂称这为“６０ａ”的肽。冻干粉经ＳｅｐｈａｄｅｒＧ－５０和羧甲基纤维素ＣＭ２２柱层析，再经反相高效液相层析纯化得最后样品，经ＳＤＳ电泳和等电聚焦电泳鉴定均为一条带，分子量约８０００，等电点在ｐＨ７．８左右；Ｎ－末端氨基酸为Ｍｅｔ；自Ｎ－末端的部分氨基酸序列为：Ｈ２Ｎ－Ｎｅｔ－Ｌｅｕ－Ｓｅｒ－Ｐｒｏ－Ａｌｓ－Ａｓｐ－Ｌｅｕ－Ｔｈｒ－Ａｓｐ－Ｉｌｅ－Ｌｅｕ－Ｐｈｅ－Ｔ     

MVD及VEGF表达与鼻咽癌侵袭转移关系的研究

为探讨微血管密度（ＭＶＤ）及血管内皮生长因子（ＶＥＧＦ）的表达与鼻咽癌（ＮＰＣ）侵袭转移关系,在分子水平干预肿瘤血管生成,预防ＮＰＣ复发和转移打下基础,采用免疫组织化学Ｓ－Ｐ法检测了７３例ＮＰＣ,１５例鼻咽良性肿瘤、２０例无瘤鼻咽部石蜡标本组织中的ＭＶＤ及ＶＥＧＦ表达,ＮＰＣ中转移组４９例,非转移组２４例。     

Detection of dynamic brain networks modulated by acupuncture using a graph theory model

Neuroimaging studies involving acute acupuncture manipulation have already demonstrated significant modulatory effects on wide limbic/paralimbic nuclei, subcortical gray structures and the neocortical system of the brain. Due to the sustained effect of acupuncture, however, knowledge on the organization of such large-scale cortical networks behind the active needle stimulation phase is lacking. In this study, we originally adopted a network model analysis from graph theory to evaluate the functional connectivity among multiple brain regions during the post-stimulus phase. Evidence from our findings clearly supported the existence of a large organized functional connectivity network related to acupuncture function in the resting brain. More importantly, acupuncture can change such a network into a functional state underlying both pain perception and modulation, which is exhibited by significant changes in the functional connectivity of some brain regions. This analysis may help us to better understand the long-lasting effects of acupuncture on brain function, as well as the potential benefits of clinical treatments.     

结肠癌根治术中经门静脉属支一次性化疗预防肝转移

目的：预防结肠癌根治术后的肝转移。方法：自1992－1994年，我们对59例结肠癌患者施行结肠癌根治术，在术中从肠系膜上静脉或肠系膜下静脉或其它任何一个门静脉属支一次性注入5．Fu1．0克。结果：术后随访3年，肝转移年6．78％。结论：结肠癌根治术中经门静脉属支一次性化疗可明显降低肝转移率。