Maternal vitamin A restriction alters biochemical development of the brain in rats

Summary The biochemical development of the fetal brain in relation to maternal vitamin A restriction was studied in rats. The vitamin A status of pregnant rats was varied by supplying low, medium and adequate amounts (6, 40, and 100 g retinol/day/kg body weight, respectively) of vitamin A during pregnancy and suckling. The maternal vitamin A restriction caused an altered brain development in terms of tissue weight, DNA, RNA and protein levels, and biosynthesis of DNA and protein from [³H]-thymidine and [³H]-leucine, respectively. A dose-dependent effect of maternal vitamin A restriction on the metabolism of DNA, RNA and protein was noticed in the developing fetal brain of rats.     

粪菌移植对肺炎克雷伯菌所致小鼠肝脓肿感染的抑制作用

探讨粪菌移植(FMT)对高致病性肺炎克雷伯菌(Klebsiella pneumoniae)所致小鼠肝脓肿(KLA)感染的抑制作用及相关机制.小鼠随机分为对照(Healthy)组、肺炎克雷伯菌(Kp)组和干预(FMT)组. Kp组小鼠经口灌胃10⁶ CFU K1血清型肺炎克雷伯菌;FMT组在给菌24 h后经口灌胃给予正常小鼠粪菌悬液(300 mg/mL,0.3 mL).结果表明,与Kp组相比,FMT处理明显降低了小鼠KLA感染率;16S rRNA高通量测序分析表明,FMT显著改变了小鼠肠道菌群组成,包括增加了Alloprevotella、norank＿f＿Muribaculaceae等益生菌的丰度;同时FMT处理明显降低了小鼠血清TNF-α和胃肠内防御素分子浓度,同时增加了肠黏蛋白2(MUC2)的表达.因此,肠道菌群和免疫防御因子可能一起参与了FMT对高致病性肺炎克雷伯菌所致小鼠KLA感染的抑制作用.     

小白鼠全脑切片^3H—GABA的摄取以及抑制物对摄取的影响

在36℃培育的小白鼠脑切片对~3H-GABA的摄取是浓度依赖性的、可饱和的,当2ml培育液中注入100μl浓度为215×10~(-4)mol/L的~3H-GABA时,脑片对~3H-GABA的摄取达到饱和。3℃时切制脑片能获得较好的~3H-GABA摄取效果。苄青霉素钾(PG)7.6×10~(-4)mol/L、苯巴比妥钠(PhB)9.8×10~(-4)mol/L、Gabaculline 4.0mol/L各100μl均使脑片对~3H-GABA的摄取明显下降,荷包牡丹碱(BiC)0.25×10~(-4)mol/L、氨氧乙酸(AOAAA)1.0×10~(-4)mol/L可使脑片对~3H-GABA的摄取明显上升。     

雌亚洲玉米螟产生性信息素的调控作用

雌亚洲玉米螟Ostrinia furnacalis仅在黑暗期产生性信息素,性信息素的产生?脑中一种活性因子的控制,光照期、黑暗期的雌蛾脑匀浆液都有这种活性因子存在,但黑暗期脑匀浆活性较高,雄蛾脑匀浆液也有这种活性,雌大蜡螟Galleria mellonella脑匀浆液也对亚洲玉米螟雌蛾有刺激活性,保幼激素类似物ZR-512对亚洲玉米螟性信息素的产生即无刺激作用也无抑制作用。切断腹部末端神经亦不影响脑因子促使性信息素的产生和释放。     

从系统科学角度探究“哲学始于惊讶”的本质

从柏拉图、亚里士多德开始就认为"哲学始于惊讶",但对此问题的研究仍比较粗浅。惊讶是广泛的,它是获得智慧的人脑机能基本过程。惊讶的本质是人脑内思维非简单和的涌现。从系统科学角度探究"哲学始于惊讶"的丰富本质,使哲学作为智慧和一般知识的关系有一个新角度的认识。     

脑脓肿42例诊治分析

目的：总结42例脑脓肿诊治经验及效果.方法：对42例脑脓肿病人15例施立体定向置管抽吸引流术,12例钻孔脓肿穿刺置管引流术,15例脑脓肿切除术..结果：治愈40例,重残1例,死亡1例.结论：CT引导立体定向穿刺置管抽吸引流术手术损伤小,操作简便,定位精确,安全可靠.     

Tetanus toxin does not affect the release of noradrenaline and taurine from rat cerebral cortex slices evoked by high K+ and Na+-free media

Summary Noradrenaline and taurine release from superfused rat cerebral cortex slices was stimulated by potassium ions, veratrine, ouabain and omission of sodium ions. Tetanus toxin enhanced only the ouabain-evoked calcium-dependent noradrenaline release and the ouabain-evoked calcium-independent taurine release. The uptake of both was marginally affected.     

凶险型颈深部脓肿的相关因素研究

探讨凶险型颈深部脓肿的相关因素。回顾性分析莆田学院附属医院耳鼻咽喉科2002年3月～2011年11月收治的8例凶险型颈深部脓肿患者的临床资料。结果,8例凶险型颈深部脓肿患者中5例痊愈,3例死亡。结果表明：当颈部脓肿累及多个颈深筋膜间隙感染,或并发糖尿病、肥胖、脓毒血症、感染中毒性休克、窒息等因素时,为凶险型颈深部脓肿;对该病的早期诊断、早期治疗,可以预防严重并发症的发生,有效降低死亡率。     

不典型肺脓肿的CT影像诊断及28例临床分析

目的探讨不典型肺脓肿的CT特点.方法重点分析28例不典型肺脓肿的CT影像表现.结果不典型肺脓肿可分为孤立团块型和不规则浸润型,且具有以下共同CT特点:局限融解或小空洞、局部充血征、边缘粗长索条影、周围局限浸润片影和邻近胸膜增厚粘连.结论明确脓腔的存在和病灶周围的炎性改变是确定诊断和鉴别的关键.     

Immunorecognition of estrogen and androgen receptors in the brain and thoracic ganglion mass of mud crab, Scylla paramamosain

The brain and the thoracic ganglion of a crustacean can synthesize and secrete gonad-stimulating hormone (GSH) which stimulates the maturation of gonad. In the previous experiments, sex steroid hormones (estradiol, testosterone, progesterone, etc.) have been detected from the crustacean. However, the feedback regulation of sex steroid hormones on the brain and the thoracic ganglion of the crustacean has not been reported so far. In the present experiment, monoclonal antibodies were applied to investigate the immunorecognition of estrogen receptor (ER) and androgen receptor (AR) in the brain and the thoracic ganglion mass of Scylla paramamosain. The results showed that the distribution of the immunopositive substances of ER and AR was extremely similar. They distributed in the protocerebrum, deutocerebrum and tritocerebrum of the brain, and mainly in protocerebrum. In the thoracic ganglion mass, immunopositive substances distributed in the subesophageal ganglion, thoracic ganglion and abdominal ganglion, and mostly in subesophageal ganglion. Immunopositive substances of ER and AR mostly existed in the cytoplasm of neurons. The present study will provide morphological evidence for the origin and the evolution of ER and AR. In addition, the immunoreactivities of ER and AR suggested that the estrogen and androgen may be involved in the feedback regulation of crustacean neuroendocrine.