Characterization of 17β-estradiol 3-(β-D-glucopyranoside) and 17-(α-D-glucopyranoside) as the metabolites of 17β-estradiol in the cultured ovaries of the silkworm,Bombyx mori

Summary The structures of the metabolites formed upon incubation of 17-estradiol with the ovaries of silkworm,Bombyx mori, have been determined as 17-estradiol 3-(-D-glucopyranoside) (1) and 17-(-D-glucopyranoside) (2) by spectroscopic means.     

膦酰基吡唑和噻二唑化合物的合成

通过膦酰基腙与Vilsmeier试剂（DMF/POCl3）以及氯化亚砜的赫德-默里反应（Hurd-Mori Reaction）,得到了1-苯基-3甲基-4-（O,O-二乙基）-磷酰基吡唑、1-苯基-3-（O,O-二乙基）-膦酰基吡唑和5-（O,O-二乙基）-膦酰基-1,2,3-噻二唑3个新化合物,产率30%-92%。其结构均经^1H NMR、^31P NMR、MS及元素分析所证实。     

家蚕后部丝腺细胞粗面内质网膜系统的多态性研究

应用电镜技术,对家蚕后部丝腺细胞粗面内质网膜系统在5龄生长期的超微形态变化,进行了连续观察与研究.结果发现,粗面内质网膜系统的超微形态呈现出扁平囊状、管泡状和同心圆状等规律性变化,这种多态性现象与丝腺细胞的生长发育状态及生理机能的变化密切相关,并受蚕体内分泌环境的调节.     

家蚕核型多角体病毒gp64基因的定位及克隆

以苜蓿丫纹夜蛾核型多角体病毒ＡｃＭＮＰＶ的膜表面糖蛋白基因ｇｐ６４作为探针，对经不同内切酶酶切的家蚕核型多角体病毒基因组ＤＮＡ进行Ｓｏｕｔｈｅｒｎ杂交分析，发现ＢａｍＨⅠ酶切产生的４．２ｋｂ和７．６ｋｂ片段呈阳性杂交，经ＤＮＡ片段回收，将４．２ｋｂ片段进行了克隆     

电磁辐照诱发家蚕变异的实验研究

电磁场辐照家蚕卵,能使家蚕产生明显的变异.比较试验的研究结果显示,经过电场刺激后的蚕种,茧质和茧量均有所提高;此外,也观察到生物大分子的局部结构变化的表现型变异.     

Effect of xanthurenic acid on P-450-dependent biotransformation by molting glands in vitro

Incubation of molting glands from the crayfishProcambarus clarkii (Y-organ) and the silkwormBombyx mori (prothoracic gland) with 23,24-[²H₄]-2-deoxyecdysone resulted in the production of deutero-ecdysone; this biotransformation was inhibited in the presence of xanthurenic acid. When the experiments were performed under an¹⁸O₂ atmosphere, the¹⁸O atom was introduced into ecdysone, as confirmed by mass spectrometry. We therefore suggest that xanthurenic acid inhibits P-450-dependent hydroxylation of 2-deoxyecdysone. However, deutero-2-deoxyecdysone was not converted to 3-dehydroecdysone when using Y-organs in vitro, although it is a major product. We therefore conclude that the biosynthetic pathway of ecdysteroids inP. clarkii branches at an early step.     

家蚕,蓖麻蚕,樗蚕,樟蚕核型研究

探讨了蚕类染色体制片方法和分带技术，分析了家蚕，蓖麻蚕，樗蚕和樟蚕的核型，并对染色体的识别问题进行了讨论。     

攀西桑粉虱的形态研究

桑粉虱Pealius mori(Takahashi)和杨梅粉虱Bemisia myricae Kuwana的一般外形特征非常相似极易混淆,为了弄清攀西地区发生的主要种类究竟是杨梅粉虱还是桑粉虱我们对其形态进行了显微研究.发现该地区危害的种类是桑粉虱,表明除已报道的台湾、江浙外,四川攀西也有桑粉虱的发生.