Molecular genetic basis for the rhino mouse from Chinese KM subcolony

Both the rhino mouse and hairless mouse resulted from hairless gene mutation, but they show different phenotypes of skin physiology. The rhino mouse has more similar histological characters to human papular alopecia.Therefore rhino mouse is a good experimental animal model for human papular alopecia. This study reports a hairless mouse named rhino KIZ, arose from KM colony in Kunming Institue of Zoology, by systematic studies on morphology,skin histopathology, gene sequence, pedigree and protein domain analysis. The results demonstrate that a C-to-T transition in exon 11 of hr gene (The mutant gene has been applied for a Chinese patent (patent No. 03135280)) results in the rhino KIZ. The rhino KIZ with clear genetic mechanism will be a useful animal model.     

绵羊角蛋白关联蛋白KAP6-1基因5′端调控区的分子克隆及测序结果比较

根据绵羊毛囊角蛋白关联蛋白(KAP 6-1)基因已知DNA序列设计合成了两个特异性引物,以绵羊基因组DNA为模板,PCR扩增出1 042 bp的特异性片段,连接到pM D 19T载体中获得该片段克隆.经过快速提质粒法筛选、限制性内切酶分析表明该克隆包含所需的目的片段.DNA测序结果也证明该克隆片段与原基因5′端调控区序列相比具有很高的一致性.研究结果为今后制备转基因克隆动物,在毛囊细胞中特异性表达绒毛生长调控基因奠定了基础.     

等厚干涉法测量应用实验研究

利用等厚干涉测量头发丝的直径,可以训练学生的实验操作技巧,加深对实验原理的掌握,丰富了实验内容,避免了实验结果的千篇一律,同时激发了学生的实验兴趣.     

Can hair width and scale pattern and direction of dorsal scapular mammalian hair be a relatively simple means to identify species?

Hair width and surface scale patterns (morphological characteristics) of hair from 21 selected species representing seven orders of mammals from the western United States were studied using scanning electron microscopy (SEM). The orders studied were Artiodactyla, Carnivora, Chiroptera, Didelphimorphia, Insectivora, Lagomorpha and Rodentia. Hair width, scale length and height, scale patterns, and scale position in relation to the shaft were used to characterize differences in morphology for both the species and orders. Specimens were taken from the dorsal scapular region and the centres of hair shafts of mature mammals were used for images. A key (identification chart) was created to characterize each species. These studies suggest that morphological differences in guard hair and underfur could provide a useful atlas for forensics, taxonomy and archaeology.     

5-Br-PADAP显色法同时测定人发中锌、铜、铁的含量

提出了在弱酸性介质及抗坏血酸和乳化剂存在下 ,以 5 -Br -PADAP为显色剂 ,选用了铜、锌、铁的最大吸收峰为 5 5 8,5 6 6和 76 0nm为测定波长 ,按系数倍率法和两波长标准加入法原理处理测量数据 ,直接测定了人发中铜、铁、锌的含量     

浅谈实验小鼠非病理性脱毛原因

随着生命科学研究的发展,动物实验中小鼠的使用频率越来越高。实验人员对实验小鼠不明原因的脱毛现象十分困扰,影响实验结果的判断,本文针对小鼠的非病理性脱毛,简述包括自然发生和偶然诱导脱毛的原因,实验人员可根据具体情况作出科学的判断。     

7个月—9岁小儿发锌含量的研究

利用原子吸收光谱对信阳市104例7个月—9岁儿童发锌含量进行了分析研究,结果表明,发锌含量与小儿年龄呈负相关,7个月—7岁小儿年龄与标准差呈负相关,这提示我们,年龄越小,发锌含量越高且越不稳定。     

朝鲜鹌鹑耳蜗的组织发生

以朝鲜鹌鹑胚胎和幼体的内耳为材料，用组织学方法对内耳的发育过程进行研究。孵化３６ｈ，听基板已形成；孵化７２ｈ，听泡形成；孵化４ｄ，基乳突形成；孵化６ｄ，毛细胞分化出来；孵化１６ｄ，内耳结构基本发育完成；出壳后，其结构继续发育完善。 发育结果显示：螺旋神经节细胞和毛细胞优先发育，附属结构在其后发育。     

Isolation and Properties of Melanin in Fine Yak Hair

Melanin granules were isolated from fine yak hair by means of protein enzyme and hydrochloric acid.Resultsof SEM investigation and amino acid analysis were usedto study the physical and chemical properties of melaningranules.The solubility of melanin granules in differentchemical solutions was also studied.     

紫露草雄蕊毛技术对水质的突变性研究初探

用紫露草雄蕊毛突变技术对盘龙江松花坝至滇池河段的水质进行监测．监测结果显示：整条江水质呈现出轻重不一的污染段面．北仓、滇池汇水口样点水质具有较高的雄蕊毛突变率，突变率分别为０．４９２‰和０．５５６‰．城区河段紫露草雄蕊毛突变率较低，最低点为双凤桥样点，突变率为０．２２６‰．但从整体来看，由松花坝雄蕊毛突变率０．０９１‰至滇池汇水口处增至０．５５６‰，水体的潜在性致突变物污染程度逐渐加重．这表明盘龙江水体可能存在致突变物污染的潜在危险性