Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system

The human adenovirus type 5 E1A, a tumor- suppressor gene[1], codes for two major related proteins of 243 amino acids (12S) and 289 amino acids (13S) by al-ternative splicing in two exons[2]. Studies have been shown that E1A can regulate expression of many genes and cell cycle[3]. Both in vitro and in vivo experiments indicated that E1A could induce tumor cells differentia-tion, convert tumor cells into an epithelial phenotype, in-hibit tumor cell growth and metastasis and strongly en-ha…     

Mechanical Studies on Treatment of Malignant Tumour by Ultralow Frequency Pulsed-Gradient Magnetic Fields

Ultralow frequency (ULF) pulsed-gradient magnetic field (with the maximum intensity of 0.6-2.0 T, gradient of 10-100 T·m-1, pulse width of 20-200 ms and frequency of 0. 16-1. 34 Hz) treatment of mice can inhibit murine malignant tumour growth and can induce apoptosis of cancer cell. The apoptotic cancer cell contracted, became rounder and divorced from adjacent cells; the heterochromatin condensed and coagulated together along the inner side of the nuclear membrane; the endoplasmic reticulums expanded and fused with the cellular membrane; many apoptotic bodies which were packed by the cellular membrane appeared and were devoured by some lymphocytes and plasma. By Lorentz force the magnetic field keeps the moving ions within bounds of Larmor radius. Thus, penetrating capability of the positive and negative ions through the cell membrane was affected, even the role on the cell membrane formed.     

Genetic Diversity in Populations of Sepiella maindroni Using 16S rRNA Gene Sequence Analysis

Part of the 16S rRNA gene is amplified with PCR and sequenced for 5 populations of common Chinese cuttlefish Sepiella maindroni:three from the South China Sea,one from East China Sea and one from Japan.The result shows that a total of 5 nucleotide positions are found to have gaps or insertions of base pairs among these individuals,and 13 positions are examined to be variable in all the sequences,which range from 494 to 509 base pairs.All of the individuals are grouped into 7 haplotypes (h1-h7).No marked genetic difference is osberved among those populations.All of the individuals from Nagasaki belong to h1 and the h3 haplotype is found only in the coastal waters of China.A→←G transition in Nucleotide 255 is suggested to be taken as a kind of genetic marker to identify the populations distributed in East-South China Sea and the Nagasaki waters of Japan.     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

关于常微分方程初值问题数值解误差的探讨

引用B样条插值函数讨论了一阶常微分方程初值问题的数值解 ,给出一个隐式近似求解公式 ,并得到此公式的局部截断误差为O(h5) ,整体截断误差为O(h4 ) .在此基础上又给出了一个校正显式求解公式 ,其局部截断误差为O(h4 ) .     

基于B/S的客户关系管理系统的设计与实现

客户关系管理系统通过对客户详细资料的深入分析来提高客户满意程度，提高企业的竞争力，是现代企业的竞争法宝．文章以SuniAIR集团公司的客户关系管理系统开发为背景。提出了基于B／S模式的客户关系管理系统。研究并设计了其体系结构。详细介绍了其主要模块功能．该系统运行效果良好，取得了预期效果．     

基于网络的多媒体课件的交互设计与实现

基于网络的多媒体课件必须具备交互性的特点，以实现学生与计算机、教师与学生的交互功能，探讨了课件人机交互、教学交互的几种交互方式的设计思路和制作方法，并阐述了基于WEB的B／S模式体系结构的课件设计技术。     

熔融碳酸盐燃料电池阴极材料制备与导电性

研究了熔融碳酸盐燃料电池（MCFC）阴极材料锂铁氧化物电极的导电性能。实验结果表明在合成锂铁氧化物的过程中，使Li2CO3轻微过量，可以提高锂铁氧化物电极的导电性。在同一温度下，随着Li:Fe值的增大，锂铁氧化物电极的导电性增加。相同Li:Fe条件下，锂铁氧化物电极的导电性随着温度升高按指数规律增加。扫描电镜研究（SEM）表明，Li:Fe比值不同，制备的锂铁氧化物电极表面形貌也不同。     

HFA3861B基带处理器的应用

介绍了一种新的直序扩频(DSSS)通信基带处理器HFA386lB的功能、特点及结构，重点分析了HFA386lB芯片的控制端口、发送数据端口和接收数据端口的工作时序，并就所设计的直序扩频系统详细阐述该基带处理器的应用，以及应用中需注意的问题．     

五阶带形状参数的B样条曲线

给出了五阶带形状参数的均匀B样条基函数，使五阶均匀B样条基函数成为它的一个特例．由带形状参数的五阶均匀B样条基构成的样条曲线，可通过改变形状参数的取值而调整曲线的形状，并且可以调整曲线接近其控制多边形的程度。