人非小细胞肺癌rasP^21和CD44的表达及意义

用免疫组化ＬＳＡＢ法检测４６例非小细胞肺癌ｒａｓＰ２１和ＣＤ４４的表达。结果：ｒａｓＰ２１的阳性率鳞癌为５９％,腺癌６３％;ＣＤ４４的阳性率鳞癌为４１％,腺癌６３％。统计学分析证明：不同临床分期的ｒａｓＰ２１表达强度有显著性差异（Ｐ＜００１）;有淋巴结转移与无淋巴结转移的ＣＤ４４表达程度有显著性差异（鳞癌Ｐ＜００１,腺癌Ｐ＜００２５）;ｒａｓＰ２１与ＣＤ４４表达强度两者无相关性（Ｐ＜００５）。结论：ｒａｓＰ２１的表达与预后不良有关,ＣＤ４４的表达与淋巴结转移有关。     

Marked electrical synapses on AP neuron of the leech (Whitmania pigra)

An initial attempt reveals parameters of marked electric synapse on AP perceptive neuron of leech. The width of gap between marked AP and either neuro-or glio-process is 2–3 nm, the diameter of the hexagonal array of connexion is 8–9 nm with a central pole of 2–2.5 nm, the same as the structure of annular lamellar bodies internalized by AP-glia processes in the AP.     

Signal transduction pathways mediated by colony stimulating factor-1 receptor

Colony-stimulating factor-1 (CSF-1), which is necessary for cell proliferation and differentiation, regulates both immediate and delayed early responses throughout G1 phase. The binding of CSF-1 to its receptor (CSF-1R) triggers phosphorylation of the receptor and its intrinsic tyrosine kinase. The activated receptor binds directly to cytoplasmic effector proteins, which induce multiple-signal transduction pathways. CSF-1 can induce the c-myc gene expression via Ras and Ets-related proteins. The expression of c-fos/jun family genes is also targeted following the activation of Ras. CSF-1R activates STAT1 and STAT3 to participate in signaling, but JAKs do not appear to contribute to signaling by CSF-1R. CSF-1R activates PI3-kinase, and PI3-ki can interact with downstream proteins by the MAPKK-related pathway independent of Ras/Raf. PC-PLC can enforce signaling in response to CSF-1. Furthermore, the turnover and dephosphorylation by the phosphatase SHPTP1 of CSF-1R are the major mechanism in the negative regulation of signaling by CSF-1R     

Neuropeptide Y receptors in a protozoaStylonychia mytilus

Radio ligand binding assays(RLBA) were used to study neuropeptide Y (NPY) receptors in a protozoa Stylonychia mytilus. The experimental results showed that 2-3×10+3/mL Stylonychia cells incubated in Pringsheim solution which contained 3H-NPY could specifically bind 3H-NPY and concomitantly present saturable characteristic. This suggested that Stylonychia possessed some specific binding sites for NPY. Scatchard transformations of binding assay for the NPY receptors at 25℃ are compatible with the specific activity of 42.47 fmol/10+3 cells and the binding equilibrium constant of 0.113 nmol/L. The data of 125I-NPY binding assay to the membrane protein extract of Stylonychia indicated that there was a significant difference between the amount of total bound and nonspecific bound of 125I-NPY. This result indicated that NPY receptors were probably localized mainly on the cell membrane.     

数字无绳电话PHS及其系统软件设计

数字无绳电话ＰＨＳ系统是结合了最新无线访问技术和智能网络技术的新的通信服务．介绍了ＰＨＳ系统的无线访问方法ＴＤＭＡ／ＴＤ和无线通道．按照通信协议的分层,设计了相应的软件结构．在物理层,实现了基本的物理帧传输;在数据链路层,实现了滑动窗口协议的数据帧传输;在网络层,实现了无线管理、移动管理、呼叫控制等．给出了这些层的典型函数模块．应用层是由中断驱动来实现各种服务的,并按优先级来调度处理事件．ＰＨＳ具有很高的性能价格比,具有广阔的应用前景．     

Endothelial Cell Culture on Different Phospholipid Surfaces *

ＩｎｔｒｏｄｕｃｔｉｏｎＢｉｏｍａｔｅｒｉａｌｓａｒｅａｌｗａｙｓｕｓｅｄｉｎａｎｅｎｖｉｒｏｎｍｅｎｔａｄｊａｃｅｎｔｔｏｌｉｖｉｎｇｔｉｓｓｕｅｓａｎｄｂｌｏｏｄ．Ｃｅｌｕｌａｒｉｎｔｅｒａｃｔｉｏｎｓｗｉｔｈｉｍｐｌａｎｔｓａｒｅｏｆｐｒｉｍａ...     

Construction of High Level Expression Cell Lines of Erythropoietin *

Ｅｒｙｔｈｒｏｐｏｉｅｔｉｎ（ＥＰＯ）ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｉｍｐｏｒｔａｎｔｒｅｇｕｌａｔｏｒｙｆａｃｔｏｒｓｄｕｒｉｎｇｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｍａｍｍａｌｉａｎｅｒｙｔｈｒｏｃｙｔｅ,ａｃｔｉｎｇｍａｉｎｌｙｏｎｔｈｅｅｒｙｔｈｒ...     

基于神经网络的无监督自学习控制系统

所论系统的控制由两个神经网络完成,即对象放识器和系统控制器。并且提出了一种 新的学习算法,它能利用存贮于辨识网络的信息改进控制策略。该控制系统可以对非线性系统,不确定系统等进行无监督的学习控制。     

老年牙周炎患者外周血NK细胞活性的研究

测定老年牙周炎患者外周血ＮＫ细胞活性。采用ＭＴＴ法测定,并与成年和青少年牙周炎患者进行比较。发现老年牙周炎患者外周血ＮＫ细胞活性低于成年牙周炎患者（Ｐ＜０．０１）,也低于青少年牙周炎患者（Ｐ＜０．０５）。老年牙周炎患者ＮＫ细胞活性低下可能是老年人牙周病发病率高,组织破坏重的原因之一。