基准面旋回对储层宏观非均质性的控制——以胡状集油田沙三中6—8油层组为例

储层层间非均质性与沉积微相和砂体发育规律关系密切,明显受控于基准面升降造成的可容空间变化和沉积物通量大小.通过对胡状集油田沙三中6—8油层组基准面旋回的识别、划分与对比,建立了该区高分辨率层序地层格架,采用砂岩分层系数、分布密度和隔层密度、频率等参数描述了各中期旋回储层层间非均质性变化,认为储层宏观非均质性受控于基准面的旋回变化,长期基准面上升的早期和下降的晚期形成的中期旋回,储层非均质性弱,长期基准面旋回由上升到下降的转换时期储层非均质性强.     

清洁生产的热力学循环探讨

通过对工业系统和热力系统的对比分析，提出运用热力学原理来诠释清洁生产的理论．在此基础上．对工业生产的三种模式和清洁生产的评价体系进行热力学解释，并将热力循环运用于清洁生产，试图从热力学原理中找到清洁生产理论的新突破。     

关于图的Ramsey数的几个下界

推广了3个C4对完全图的R am sey数下界以及一个经典R am sey数下界问题,得到了3个C4对完全图的R am sey数的线性下界,以及一个关于多项式的经典R am sey数下界.     

冷备可修系统的二元最优更换策略

研究了由一个修理工、两同型部件组成的冷贮备可修系统 ,在假定故障部件不能“修复如新”的条件下 ,利用几何过程对系统中部件 1的故障次数N和工作寿命T组成的二元最优更换策略 (N ,T)进行了研究 ,问题是确定最优的更换策略 (N ,T )使得系统经长期运行单位时间内期望效益达到最大。导出了系统经长期运行单位时间内期望效益的明显表达式。最后 ,还在一定条件下证明了最优策略 (N ,T )比以往著作中的最优策略N 和T 优良。     

基于语义网络的方案设计过程表达与推理

从基于机械运动方案设计的表达方法必须能支持概念设计全过程的思想出发，提出计算机辅助概念设计系统必须能够表达设计方案、产生方案、产生多个方案和确定最优方案．根据方案表达方法应具备的基本功能要求，选用语义网络技术表达设计方案过程、工艺动作、机构及其相互间的关系，通过对设计方案中机构时间配合及其影响的表达和调整实现了方案设计运动循环图表达的计算机辅助化．利用基于语义网络表示的不确定性推理技术构造待求问题的语义网络片断，通过已有设计实例与设计要求的属性值与属性值、属性值与属性值域、属性值域与属性值域三者的匹配比较构造贴近度计算公式，并对工业平缝机中的挑线机构进行了机构选型．实例证明了击模型的正确、实用性．     

百色盆地百49块百岗组三段高分辨率层序地层学分析

以高分辨率层序地层学理论为指导，通过地表露头、岩芯、测井资料的综合研究，并结合地震剖面资料，分析了百色盆地塘寨油田百49块古近系百岗组三段不同级别的层序界面和湖泛面特征及识别标志，划分出1个长期、3个中期、14个短期基准面旋回层序。在此基础上，详细讨论了各级次基准面旋回层序的垂向序列、岩性岩相组合、结构类型及更加样式等特征，并探讨了高分辨率层序地层学的研究成果在区块油气开发工程中的应用前景。     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system

The human adenovirus type 5 E1A, a tumor- suppressor gene[1], codes for two major related proteins of 243 amino acids (12S) and 289 amino acids (13S) by al-ternative splicing in two exons[2]. Studies have been shown that E1A can regulate expression of many genes and cell cycle[3]. Both in vitro and in vivo experiments indicated that E1A could induce tumor cells differentia-tion, convert tumor cells into an epithelial phenotype, in-hibit tumor cell growth and metastasis and strongly en-ha…     

东营凹陷沙三段-沙二下亚段T-R层序特征及成因

将东营凹陷古近系沙三段沙二下亚段划分为 3个三级层序 ,即沙三下层序 (SSⅠ )、沙三中层序 (SSⅡ )和沙三上沙二下层序 (SSⅢ )。SSⅠ和SSⅡ层序由湖进体系域和湖退体系域组成T R旋回 ,其特征是代表层序边界的不整合分布局限 ,湖进体系域厚度小 ,主要为细粒沉积物 ,退积式叠加 ;湖退体系域较厚 ,由进积式叠加的砂岩、砾岩和泥岩组成。T R层序的形成主要是由于层序形成早期的构造沉降和气候起主导作用 ,其基底快速沉降 ,湖水供应充分 ,湖平面快速上升。在层序形成晚期 ,沉积物供给和气候起主导作用 ,沉积物供给多 ,快速向前推进 ,湖平面下降。构造沉降和沉积物供给因素对层序控制作用的转换促使其形成层序和体系域的边界。T R层序与三分层序之间的转换是由于层序形成的不同阶段的主控因素不同而引起的     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.