浅谈现场总线技术

介绍了一种新兴的数据总线--现场总线,并阐述FCS的发展.     

Passivation method of 317L stainless steel used in medicine

The electrochemical performance of 317L stainless steel used in medicine under different conditions of passivation (different contents of HNO₃ solution, different passivation time and different passivation temperatures) was studied. The results show that the pitting potential of 317L stainless steel used in medicine can reach about 1.0 V (SCE) when electrochemically tested in 0.9% NaCl solution after the steel was passivated in 30% HNO₃ solution at 35℃ for 6 h, which indicates that the passivation film has a relatively strong resistance to corrosion. The results also show that the corrosion resistance of the passivation film on the surface of 317SS can be increased after suitable amount of K₂Cr₂O₇ is added into HNO₃ passivation solution.     

欧洲七叶树体细胞次生胚重复发生研究

取欧洲七叶树体细胞胚的子叶诱导次生胚发生,表明:MS ZT(0.5~2.0 mg/L) BA 1.0 mg/L IAA 0.2 mg/L培养基可直接诱导次生胚发生,诱导率超过80%;同一子叶的不同部位分化能力不同,靠近胚轴部位分化能力最强,其他部位比较弱。     

316L不锈钢粉末的电子束选区熔化成形

电子束选区熔化技术在人体植入物、航空航天小批量零件的直接快速制造方面具有重要的意义。为此,该文采用自行研制的电子束选区熔化设备,研究了电子束选区熔化316L不锈钢粉末的工艺参数、微观组织及熔化成形机理。结果表明:电子束电流、作用时间和聚焦电流对层间结合情况影响较大,作用时间和填充线间距对可成形性影响较大;当能量密度系数为62.8 GJ/m3、基板厚度为0.5mm时,制备的成形件晶粒细小,组织均匀,无气孔、裂纹及未熔颗粒等缺陷出现。     

Inhibitory Kinetics of p-Substituted Benzaldehydes on Polyphenol Oxidase from the Fifth Instar of Pieris Rapae L.

Polyphenol oxidase (PPO) is the enzyme responsible for enzymatic browning during the growth of insects. It is also involved in defense reactions and is related with immunities in insects. PPO, a metalloenzyme oxidase, catalyzes the oxidation of ο-diphenol to ο-quinone. The present paper de- scribes the effects of benzaldehyde and its p-substituted derivatives on the activity of PPO from the fifth instar of Pieris rapae L. PPO from the fifth instar of Pieris rapae L. was purified using ammonium sulfate fractionation and chromatography on Sephadex G-100. The enzyme kinetics was characterized using L-3,4-dihydroxyphenylalanine (L-DOPA) as substrate. The results show that benzaldehyde, p- hydroxybenzaldehyde, p-chlorobenzaldehyde, and p-cyanobenzaldehyde can inhibit the PPO activity for the oxidation of L-DOPA. The inhibitor concentration leading to 50% activity lost, IC50, was esti- mated to be 5.90, 5.62, 2.83, and 2.91 mmol/L for the four tested inhibitors, respectively. Kinetic analy- ses show that the inhibitory effects of these compounds are reversible. Benzaldehyde, p- hydroxybenzaldehyde, and p-chlorobenzaldehyde are noncompetitive inhibitors while p-cyano- benzaldehyde is a mixed-type inhibitor. The inhibition constants were determined for all four inhibitors. p-chlorobenzaldehyde and p-cyanobenzaldehyde were more potent inhibitors than the other com- pounds. These results provide a basis for developing PPO inhibition-based pesticides.     

生长调节剂对万寿菊花药培养的影响

以B5为基本培养基,选用KT／NAA、KT／2,4-D、6-BA／NAA、6-BA／2,4-D4种生长调节剂组合;每种组合设20／3、25／3、50／3、80／3、50／1、80／16种比例,研究对万寿菊花药愈伤组织诱导和器官分化的影响。结果表明,KT／NAA=20／3的花药愈伤组织诱导率最高;KT／NAA：25／3、KT／NAA=20／3和6-BA／NAA=25／3最有利器官分化;2,4-D与6-BA、KT组合对器官分化无作用。     

青海早熟禾属植物系统修订

本文通过对青海省早熟禾属植物的研究,提出了一个青海省早熟禾属分类系统排列,首次确认青海省产10组45种,其中8种为青海新记录种类,2种青海特有种．     

花粉管通道法转基因技术在甜瓜品种河套蜜瓜上的应用

为建立甜瓜(Cucumis melo L.)简便易行转化频率高的转基因技术,以甜瓜品种河套蜜瓜为受体材料,以含gus基因的植物双元表达载体pPZP221为外源基因供体,进行了花粉管通道法转基因研究.自交授粉后分别于1、2、3、4、5、6、7、8、9、10 h,切去柱头上端1/3部分,立即滴加质粒DNA溶液,果实成熟后收获转化种子.对T0代植株进行PCR检测,结果表明不同时间处理所得到的T0代植株均具有较高的转化频率,其中授粉后7 h滴加DNA溶液所获得的转化率为28.3%.对一部分PCR阳性的T0代植株基因组DNA进行Southern杂交分析,结果表明所有样品均出现特异性杂交条带,证明外源基因已整合到受体植物基因组中.     

不同地区银杏花粉黄酮和内酯含量的差异性

以15个不同地区百年左右的银杏雄树花粉为材料,用高效液相色谱法(HPLC)检测了银杏花粉中黄酮和内酯的含量.结果表明:银杏花粉主要含有以山奈素为母核的黄酮醇苷,以槲皮素和异鼠李素为母核的黄酮苷的含量相对较少,银杏花粉总黄酮含量平均为20.44 mg/g;还发现银杏花粉中的内酯成分为银杏内酯A,其含量平均在2.22 mg/g;不同地区间银杏花粉之间的黄酮和内酯含量差异显著.相关分析表明,不同地区银杏花粉之间黄酮和内酯含量的差异与气候地理因子的相关性不显著.初步筛选出黄酮和内酯含量较高的重庆01、广德01、北京01、康县01等4个单株作为备选的优良单株.     

农杆菌介导人肝再生增强因子转化河套蜜瓜的研究

利用三亲融合法将含有人肝再生增强因子(hum an augm en ter of liver regeneration,hALR)的果实特异性表达载体pPZP-CAN导入农杆菌LBA 4404,转化河套蜜瓜子叶外植体,经诱导与筛选获得了抗性再生植株,PCR扩增、PCR-Sou thern和斑点杂交检测证明hALR已整合入河套蜜瓜再生植株基因组中.