嗜水气单胞菌蛋白质组分子解剖图谱的初步建立

采用蛋白质组学技术,建立了嗜水气单胞菌PPD(134/91)在稳定期的蛋白质组分子解剖图谱.将嗜水气单胞菌接种于LB培养基28℃培养18h,取菌总蛋白进行2 DPAGE.从2 DPAGE图谱中随机选取124个蛋白质点进行肽质量指纹图谱鉴定,检索发现其中10个确定为数据库中收录的嗜水气单胞菌的基因或蛋白,其比例约为1/12.实验结果对嗜水气单胞菌菌株之间的比较研究和功能蛋白质组研究具有重要参考价值.     

一株新耐冷菌SA-8降解有机磷农药的研究

从东北地区受农药长期污染的土壤中通过富集培养、分离筛选到一株甲基对硫磷高效降解菌SA-8,根据生理生化特征,初步鉴定为邻单胞菌Plesiomonas sp..菌株降解甲基对硫磷的最适温度和pH分别为20℃和7.0,在此条件下培养24h,降解率达93%;当温度位于10℃时,降解率也有66.2%;而在35℃时降解率仅为27.3%.这些结果表明SA-8是一株耐冷有机磷农药降解菌.     

Effects of temperature,hypoxia, ammonia and nitrate on the bleaching among three coral species

Coral bleaching, which is defined as the loss of colour in corals due to the loss of their symbiotic algae (commonly called zooxanthellae) or pigments or both, is occurring globally at increasing rates, and its harm becomes more and more serious during these two decades. The significance of these bleaching events to the health of coral reef ecosystems is extreme, as bleached corals exhibited high mortality, reduced fecundity and productivity and increased susceptibility to diseases. This decreased coral fitness is easily to lead to reef degradation and ultimately to the breakdown of the coral reef ecosystems. Recently, the reasons leading to coral bleaching are thought to be as follows: too high or too low temperature, excess ultraviolet exposure, heavy metal pollution, cyanide poison and seasonal cycle. To date there has been little knowledge of whether mariculture can result in coral bleaching and which substance has the worst effect on corals. And no research was conducted on the effect of hypoxia on corals. To address these questions, effects of tem- perature, hypoxia, ammonia and nitrate on bleaching of three coral species were studied through examination of morphology and the measurement of the number of symbiotic algae of three coral species Acropora nobilis, Palythoa sp. and Alveopora verrilliana. Results showed that increase in temperature and decrease in dissolved oxygen could lead to increasing number of symbiotic algae and more serious bleaching. In addition, the concentration of 0.001 mmol/L ammonia or nitrate could increase significantly the expulsion of the symbiotic algae of the three coral species. Except for Acropora nobilis, the numbers of symbiotic algae of other two corals did not significantly increase with the increasing concentration of ammonia and nitrate. Furthermore, different hosts have different stress susceptibilities on coral bleaching.     

小球藻的筛选和异养培养

从北京清河筛选出了具有异养能力的一株藻类,经初步鉴定为小球藻.在异养批量培养条件下,研究了不同氮源和碳氮比对筛选小球藻生长的效应.当葡萄糖作为惟一碳源时,硝酸钾和尿素都可以分别作为惟一氮源支持小球藻快速持续生长,而氯化铵作为惟一氮源时因使培养物中的pH快速降低而抑制了小球藻的进一步生长.当葡萄糖和硝酸钾分别作为小球藻生长的惟一碳源和氮源时,在碳氮质量比从5到20范围内,小球藻的生长随碳氮质量比的升高而明显增加,最大OD680nm达到了73.8.     

136例腹泻病人粪便弧菌科细菌的检测

目的：了解粪便中弧菌科细菌的检测，引起临床工作者的高度重视．方法：对136例腹泻病人粪便进行细菌培养、生化鉴定及药敏试验．结果：在136例腹泻病人粪便中分离出气单胞菌属16株，占11．8％，其中25％菌株是嗜水性气单胞菌．结论：弧菌科的细菌已成为当地重要的肠道致病菌．     

厦门岛陆地蜗牛自然感染吸虫幼虫期种类的调查研究

陆地蜗牛是可供多种重要寄生虫作为中间宿主的种类,其中对人及牛羊等经济动物危害严重的双腔科吸虫即是需要陆地蜗牛作为它的第１中间宿主（唐仲璋和唐崇惕１９７５［１］,１９７７［２］１９７８［３］）,也是寄生在鸟类和哺乳类的短咽科吸虫、寄生在两栖类的半肠科吸...     

奇瘿蚊新亚科新属新种记述(双翅目:瘿蚊科)

记述发现于浙江龙王山保护区的瘿蚊科(Cecidomyiidae)新属新种一中华奇瘿蚊Thaumacecidomyiasinica Yandg,g.et sp.n.,讨论瘿蚊科的高级分类阶元,建立奇瘿蚊新亚科(Thaumacecidomyiinae,subfam.n.)。     

浙江蝴蝶研究.Ⅰ.(蝶类、蛱蝶科)

本文记述线蛱蝶属一新种,浙江线蛱蝶Limenitis zhejiangensis Zhou.sp.nov.模式标本采自浙江、龙泉,保存于浙江自然博物馆。     

油为部分碳源发酵生产头霉素C的初步研究

以豆油为部分碳源进行头霉素Ｃ发酵,有利于提高产素能力。当豆油含量为７ｇ／Ｌ时是淀粉为唯一碳源时的１．２３倍,发酵过程研究表明种龄、通风量及发酵过程ｐＨ的变化对产素影响明显。     

内蒙古东部新巴尔虎右旗泡状肝色虫病原种类及流行学调查

报道1998-1999年在内蒙古东部呼伦贝尔盟新巴尔虎右旗3个乡草场进行人兽共患泡状蚴病病原调查研究的结果。沙狐（Vulpes corsac)和布氏田鼠（microtus brandti）分别感染有多种泡状肝包虫的成虫和幼虫期。其中有西伯利亚棘球绦虫（Echinococcus sibiricensis)和多房棘球绦虫（Echinococcus multilocularis)，并经人工感染试验实它们是具不同发生学的两个独立种类。布氏田鼠的泡状蚴平均感染高达2%-19.023%，在不同地点和不同季节它们的感染率有所差别。同时，在该划原布氏田鼠尚发现与其他棘球蚴不同的呼伦贝尔泡状蚴新种（Alveolaris hulunbeierensis sp.nov.)。