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梁惠珠 《曲靖师范学院学报》1988,(3)
关于一摩尔葡萄糖彻底氧化后产生多少摩尔ATP的问题,在有关的不同书中提法不一。笔者认为,在教学中阐述此问题时,不能只讲36ATP或只讲38ATP,而应分明情况。即在真核细胞中,可能有两种情况:一种是在发生磷酸甘油穿梭时为36ATP;另一种发生苹果酸穿梭时为38ATP。而在原核细胞中应为38ATP。 相似文献
3.
Synergistic effect of acute hypoxia on flow-induced release of ATP from cultured endothelial cells 总被引:4,自引:0,他引:4
Human umbilical vein endothelial cells (HUVECs) in primary cultures were perfused under normoxic or hypoxic conditions. These cells were stimulated twice for 3 min by increased flow (from 0.5 to 3.0 ml/min). Under hypoxic conditions the basal release of ATP was the same as under normoxic conditions, but during increased flow the release was greater (0.58±0.07>0.32±0.04 pmoles/ml/106 cells (+78%), for the first period of stimulation; 0.39±0.05>0.22±0.03 pmoles/ml/106 cells (+79%) for the second period). Further experiments with sequential increments in flow rate showed that under both normoxic and hypoxic conditions, a positive correlation existed between ATP release and the rate of flow but there was always more ATP released under hypoxic conditions regardless of the flow rate.HUVECs in secondary culture (second passage) were similarly stimulated. No differences were observed between normoxic and hypoxic conditions. In both cases, the quantity of ATP released during high flow (0.050±0.004 pmoles/ml/106 cells) was significantly smaller than the quantity of ATP released during low flow (0.09±0.01 pmoles/ml/106 cells).To conclude, since hypoxia alone did not affect ATP release, there appears to be a synergistic relationship between increased shear stress and hypoxia in the stimulation of ATP release from HUVECs. Moreover, the release of ATP under these conditions seems to be a property of highly differentiated endothelial cells. 相似文献
4.
种子的萌发过程受到ATP含量的调控,ATP水平是种子活力极灵敏的生化指标.而PEG(聚乙二醇6000)处理也可促进与种子萌发有关的生理生化过程。测定不同活力花生种子的ATP含量以及探讨经PEG处理后花生种子的能量变化,将有助于了解种子的劣变机理以及PEG处理促进种子萌发的作用. 1.材料和方法供试种子花生“粤油551”,1983年春植及秋植种子,由广东省农科院提供.放在干燥器中室温贮藏.人工老化的种子是在高温(32℃)和高湿(90%R.H.或100% 相似文献
5.
Ehrlich carcinoma and EL-4 thymoma ascites cells were subjected in vitro to heat shock, ATP depletion, oxidative stress, Ca2+ overlading and iodoacetamide treatment. After the transient stresses, Triton (X-100)-insoluble TIS) fractions were isolated from the cells and analysed by electrophoresis and immunoblotting. All stresses used caused rapid aggregation of cell proteins. This was manifested in a signficant rise in protein content in the TIS fractions. The protein increase was mostly due to and increase in the insolubility of actin, 57 kDa protein of intermediate filaments, 70 kDa heat shock protein (HSP 70), and some specific proteins whose insolubilization was a characteristic sign for each type of cell injury. Different survival rates in the cell lines after either stress corrlated well with differences in their TIS protein accretion. Possible mechanisms for stress-induced protein aggregation and its relationship with cell viability are suggested. 相似文献
6.
黄振炎 《温州大学学报(自然科学版)》2003,24(2):16-19
可溶性糖,单糖如葡萄糖、果糖,双糖如麦芽糖、乳糖等,对以它们和丙酮为底物的B—Z反应受ATP干扰进行研究.ATP的加入总的结果是延缓了反应的进行,即使振荡反应诱导期增长,周期变大.对这些非线性动力学行为可由FKN机理以合理的解释. 相似文献
7.
Summary We review here the present knowledge about the pathway of import and assembly of porin into mitochondria and compare it to those of other mitochondrial proteins. Porin, like all outer mitochondrial membrane proteins studied so far is made as a precursor without a cleavble signal sequence; thus targeting information must reside in the mature sequence. At least part of this information appears to be located at the amino-terminal end of the molecule. Transport into mitochondria can occur post-translationally. In a first step, the porin precursor is specifically recognized on the mitochondrial surface by a protease sensitive receptor. In a second step, porin precursor inserts partially into the outer membrane. This step is mediated by a component of the import machinery common to the import pathways of precursor proteins destined for other mitochondrial subcompartments. Finally, porin is assembled to produce the functional oligomeric form of an integral membrane protein wich is characterized by its extreme protease resistance. 相似文献
8.
用pH电位滴定法测定了Zn2+,Cu2+,Ni2+和Co2+与氨基酸水杨醛席夫碱及ATP形成的三元配合物的稳定常数,发现logβ111与pk2之间存在很好的线性自由能关系,研究了三元配合物中配体间的疏水相互作用. 相似文献
9.
用pH电位滴定法测定了Zn^2+,Cu^2+,Ni^2+和Co^2+与氨基酸水杨醛席夫碱及ATP形成的三元配合物的稳定常数,发现log β111与pk2之间存在很好的线性自由能关系,研究了三元配合物中配体间的疏水相互作用. 相似文献
10.
用pH电位滴定法测定了水溶液中二元配合物ML2+和三元混配配合物M(ATP)L2-的稳定常数,将结果与氨的相应值比较,发现所研究三元混配配合物中吡啶类配体的芳环与ATP的嘌呤环之间存在着分子内堆积作用,这种作用与中心金属离子的配位层结构有关 相似文献