Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-γ

Five cell types recently isolated from the bovine corpus luteum differed in their epithelioid morphology and their cytoskeleton, but shared common criteria of microvascular endothelial cells^1,2. To give strong evidence for the separate entity, the growth rate of the 5 phenotypically different cells was studied. They were seeded at low density on day 0. Most of these cells were treated with 200 to 1000 U recombinant bovine interferon- (IFN-) for 3 days. The untreated remainder served as controls. Cell counts were made for all cultures on days 4, 7, 10 and 13. morphology: 13 d after treatment with IFN- senescent cells as well as intact cells occurred in cultures of cell types 1 to 4. Cultures of cell type 5 were apparently unchanged and resembled their untreated counterparts. Desminpositive cells in cultures of cell type 2 developed cell processes. Growth rate: In the absence of IFN-, the growth rate was high for cell types 3 and 4, moderate for cell type 1, and low for cell types 2 and 5. The presence of IFN- caused anti-proliferative effects. These were higher for cell types 3 and 4 than for cell types 1 and 2. IFN- could be cytotoxic on cell type 3. In contrast, the cytokine tended to support the cell growth of cell type 5. These findings substantiate the postulate that endothelial cells exhibiting separate morphology in culture also function differently.     

Clifford半群的平坦性刻划

半群Ｓ称为Ｃｌｉｆｏｒｄ半群,如果它是群的强半格．利用左Ｓ１系的平坦性给出了Ｃｌｉｆｏｒｄ半群的特征刻划．     

由单片机AT89C51构成的定时控制系统

介绍了单片机ＡＴ８９Ｃ５１,给出了由ＡＴ８９Ｃ５１构成的定时控制系统,并将此系统与ＣＰＵ８０３１构成的单片机系统比较,具有结构简单、可靠性高的特点．     

含双二苯基膦戊烷铜(Ⅰ)配合物的合成与性质

室温,氮气氛下,铜（Ⅱ）盐和双二苯基膦戊烷（ｄｐｐｐ５）在溶液中通过还原取代反应合成了８种铜（Ⅰ）配合物（１．［Ｃｕ（ｄｐｐｐ５）］２（ＢＦ４）２,２．［Ｃｕ２（ｄｐｐｐ５）２（Ｃ８Ｈ３Ｏ２ＳＦ３）２］,３．［Ｃｕ２（ｄｐｐｐ５）３］（ＣＣｌ３ＣＯＯ）２,４．［ＣｕＢｒ（ｄｐｐｐ５）］２,５．［Ｃｕ２（ｄｐｐｐ５）Ｃｌ２］,６．［Ｃｕ２（ｄｐｐｐ５）３］（ＣＨ３ＣＯＯ）２,７．［Ｃｕ（ｄｐｐｐ５）（ＮＯ３）］,８．［Ｃｕ２（ｄｐｐｐ５）３］（ＣｌＯ４）２）。通过元素分析、红外光谱、热重、摩尔电导测定对其进行表征,表明这是一种行之有效的有机膦配合物的合成方法。     

盐胁迫下Na^＋,K^＋,Cl^—对碱蓬和玉米离子的吸收效应

用ＮａＣｌ、ＫＣｌ、ＮａＮＯ３和ＫＮＯ３处理碱蓬（Ｓｕａｅｄａｓａｌｓａ）和玉米（Ｚｅａｍａｙｓ）幼苗,发现ＮａＣｌ胁迫引起Ｋ＋、Ｃａ２＋、Ｍｇ２＋、Ｆｅ２＋、Ｚｎ２＋、ＮＯ－３等必需元素的含量降低．认为ＫＣｌ和ＫＮＯ３处理引起碱蓬幼苗幼叶黄化的主要原因是Ｎａ＋的缺乏;ＫＣｌ处理引起玉米和碱蓬幼苗的干枯死亡主要是由于植物体内高浓度Ｃｌ－引起的;Ｎａ＋是引起盐生植物碱蓬叶片肉质化的重要因子;Ｃｌ－是刺激碱蓬生长的重要因子     

Stability of the Sarcin/Ricin domain of 28S RNA in rat ribosome under hydrostatic pressure

Under 2 100 bar of hydrostatic pressure, KNA N-glycosidase can still cleave the N-C bond of adenosine at position 4324 in R/S domain of 28S RNA in rat ribosome, demonstrating that high pressure has no effect on the tertiary structure of S/R domain.     

Preparation and optical properties of solid solution semiconductor (Zn x Cd1−x S) doped silica glasses

Semiconductor(Zn_xCd_1−x S) doped silica glasses were prepared by Sol-Gel process andin situ growth technique. The structure of the materials was characterized by X-ray diffraction technique, the particle size of semiconductor crystallites from X-ray patterns was estimated less than 10 nm. From absorption spectra, it is obtained that the absorption edges shifted to short wavelength direction when Zn contents increased, and the absorption edge can be adjusted from 2.46 eV to 2.96 eV by controlling Zn contents. The third-order nonlinear optical susceptibility was studied at 532 nm with 8 ns pulse laser by degenerate four wave mixing (DFWM) technique.     

大洋多金属结核中五氧化二磷分析方法

试样经ＮａＯＨ溶融,水浸取后,使Ｆｅ、Ｍｎ、Ｃｕ、Ｃｏ、Ｎｉ等共存元素形成氢氧化物沉淀与磷分离,滤液中的磷用磷钼黄光度法测定。方法简便、快速、精密度好、准确度高。经对标准物质ＧＢＷ０７２４９测定,结果与推荐值吻合,ＲＳＤ（ｎ＝１０）〈１．４％。     

4种植物生长物质及温度胁迫对绿豆黄化幼苗肌醇磷脂代谢的影响

利用放射性标记和薄层层析（TIC）分离发现2,4－D,GA3和6－BA可不同程度地促进肌醇－1,4,5－三磷酸（IP3）水平的增加．在最适浓度下的作用效果是：GA3＞2,4－D＞6－BA．但脱落酸（ABA）没有引起IP3含量的变化．低温（0℃）和高温（35℃）胁迫亦引起IP3含量的增加．2,4－D,GA3,6－BA和温度胁迫的共同处理对IP3含量的增加有协同作用．表明2,4－D,GA3,6－BA和温度胁迫促进绿豆黄化幼苗肌醇磷脂代谢,ABA也许不参与绿豆黄化幼苗肌醇磷脂代谢．     

7,8-二甲基-3,6-二苯基-5-氧-8-氮螺[2.6]壬-4,9-二酮的合成及其晶体结构

利用手性源（2R,3S）－3,4-二甲基-2-苯基-1,4-氧氮杂-5,7-二酮（1）合成了具有光学活性的7,8-二甲基-3,6-二苯基-5-氧-8-氮螺[2．6]壬-4,9-二酮（3）．测定了化合物（3）的晶体结构,并讨论了在环加成反应中的不对称诱导作用的机理．