Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR

The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.T...     

2株甲型肝炎病毒蛋白酶2A核苷酸序列的测定与比较

从甲型肝炎病毒２个不同株感染的ＫＭＢ１７细胞中提取总ＲＮＡ,经ＲＴ－ＰＣＲ特异性扩增出此２毒株病毒蛋白２Ａ基因,将２个２Ａ基因克隆到ｐＧＥＭ－Ｔ载体上,经ＤＮＡ序列测定,得到２个２Ａ基因的核心苷酸序列,序列比较发现它们及与ＨＡＶ野毒株相对应序列有突变存在,Ｈ２株与ＨＭ１７５相比有一个点突变,同源性为９９．７２％;与ＨＭ１７５比较,Ｈ２株和Ｌ８株第３１９７位核苷酸均由Ａ突变为Ｇ,相应的氨基酸由丝氨酸     

玉米矮花叶病毒原的生物学鉴定

对我国西北的玉米矮花叶病毒Ｂ株系（ｍａｉｚｅ ｄｗａｒｆ ｍｏｓａｉｃ ｖｉｒｕｓ－Ｂ,ＭＤＭＶ－Ｂ）进行了系统的鉴定。ＭＤＭＶ－Ｂ侵染玉米（Ｚｅａ ｍａｙｓ）、高粱（Ｓｏｒｇｈｕｍ ｖｕｌｇａｒｅ）和狗尾草（Ｓｅｔａｒｉａ ｖｉｒｉｄｉｓ）等禾 本科杂草,但不侵染普通烟（Ｎｉｃｏｔｉａｎａ ｔａｂａｃｕｍ）、心叶烟（Ｎｉｃｏｔｉａｎａ ｇｌｕｔｉｎｏｓ）、苋色藜（Ｃｈｅｎｏｐｏｄｉｕｍ ａｍａｒ     

鸡传染性法式囊病毒VP2原核表达蛋白的纯化和复性

对原核表达的鸡传染性法式囊病毒(IBDV)VP2蛋白进行可溶性分析与不可溶性分析,结果表明蛋白主要以包涵体形式存在,利用溶菌酶、Triton-100、尿素等试剂进行纯化和复性后,对复性前后的蛋白分别与特异性多抗进行Dot-ELISA,复性后蛋白的反应活性增强了10倍,对复性后的蛋白与IBDV16株单抗进行Dot-ELISA,其中有11株与其发生特异性反应.     

计算机网络病毒传播的概率模型

研究网络拓扑对病毒传播的影响.提出在双星图上计算固定概率(病毒成功蔓延整个网络的概率)的教值方法,并成功将其转化为求解线性方程组的问题.通过仿真发现:1)双星网络越不平衡,病毒越容易在其中蔓延;2)让网络规模趋于无穷大,固定概率收敛.固定概率还可以简单地作为判断双星网络是否安全可信的参考指标.     

The translocation motif of hepatitis B virus improves protein vaccination

Cell-penetrating peptides (CPPs) have been shown to improve antigen loading of dendritic cell vaccines. Here we asked whether fusion of a CPP to a protein improves its immunogenicity when this fusion protein is directly applied as vaccine. We used the cell-penetrating translocation motif (TLM) derived from the hepatitis B virus, because no size limitation of cargos has been observed. Increased immunogenicity was observed when TLM was fused to ovalbumin (TLM-ova). TLM-ova was found to be superior to ova in inducing proliferation and cytotoxicity of ova-specific CD8+ T cells in vitro and in vivo. Using ovalbumin-expressing thymoma cells (EG7-ova), an improved anti-tumor immune response was observed for TLM-ova vaccination versus vaccination with ova. Moreover, TLM-ova vaccination induced a higher titer of anti-ovalbumin IgG2a antibodies compared to ova. These data demonstrate that CPP-protein vaccines can improve cellular as well as humoral immune responses. Received 16 November 2005; received after revision 12 December 2005; accepted 10 January 2006 †These authors contributed equally to this work     

转乙肝病毒表面抗原基因烟草发根的获得

构建了乙肝病毒表面抗原基因（HBsAg）植物表达载体,通过冻融法将HBsAg 基因转入到发根农杆菌LBA1314中,采用叶盘法将HBsAg基因导入到烟草中,获得了转基因烟草发根.对转基因烟草发根的GUS检测结果表明：转HBsAg基因烟草发根可以染成蓝色,而非转基因烟草的根没有染色反应.这说明gus基因在转HBsAg基因烟草发根获得了表达.     

病毒性肝炎病人心理分析和护理

本文对一年我们病房２６例各型肝炎病人的心理状态与特征进行了系统和分析并提出了护理措施。