Interaction of Stathmin-Like 2 Protein with the APP Intracellular Domain

Amyloid precursor protein （APP） plays a critical role in the formation of Alzheimer＇s disease （AD）. The intracellular domain APP （AID） was suggested to cause neurotoxicity in the nucleus. To investigate the functions AID, yeast two-hybrid screening was performed to identify the proteins which interact with AID. The human brain cDNA library was screened using pGBKT7-AID as a bait, and several positive clones were identified. One of them encodes a part of the human stathmin-like 2 protein （STMN2）. The interaction between STMN2 and APP implies that STMN2 may have an important function in APP-mediated AD formation.     

风机盘管温度控制器的设计

设计风机盘管温度控制器,采用特大屏幕液晶显示,通过温控器内部的NTC温度传感器,检测室内温度,并实时地与用户所设定温度进行比较,自动调节冷暖气的进气量和开启或关闭管道电动阀,达到了保持室内恒温的目的.给出了系统硬件设计及软件实现方法.     

异黑颗粒对APP/PS1双转基因模型小鼠学习记忆和抗氧化能力的影响

 为了观察异黑颗粒对小鼠行为学习及抗氧化能力的影响,选用APP/PS1双转基因模型小鼠,将其随机分成5组,另取同窝阴性小鼠作为正常对照组,每组8只,3个月龄后开始给药.给药6个月后,通过行为学实验测试小鼠学习记忆能力的变化,采用分光光度法测定脑组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性.Morris水迷宫实验结果表明,APP/PS1双转基因模型小鼠逃避潜伏期较正常组明显延长,在原象限停留时间明显缩短;异黑颗粒组小鼠逃避潜伏期较模型组明显缩短,在原象限停留时间明显延长.APP/PS1双转基因模型小鼠脑组织GSH-Px、SOD活力下降;异黑颗粒治疗组学习和记忆能力升高,脑组织GSH-Px、SOD活力升高,与阿尔茨海默病模型组比较有显著性差异.研究表明,APP/PS1双转基因模型小鼠9个月后发现有明显学习记忆功能障碍伴有抗氧化能力下降,异黑颗粒对阿尔茨海默病模型小鼠学习记忆障碍具有明显改善作用并且可剂量依赖性的提高阿尔茨海默病模型小鼠的抗氧化能力.     

What can false memory tell us about memory impairments in Alzheimer’s disease?

The range of memory impairments associated with Alzheimer’s disease (AD) has been a focus for psychological and clinical re-searchers for many years. In addition to investigations of AD patients’ veridical memory using traditional recognition memory tasks, a number of recent studies have focused on false memories to reveal the underlying causes of memory impairment in AD. Studies comparing illusory memories between AD patients and healthy older people have revealed various differences in memory deficits between the development of AD and the typical aging processes. Here, we review 3 types of memory illusions tested in AD patients: associative memory illusions, fluency-based false memories and source memory errors. By comparing AD patients with healthy older adults, we sought to analyze the mechanisms underlying AD-related memory impairments at different stages of memory processing, including encoding, retrieval and monitoring. This comparison revealed that AD patients exhibit an impaired ability to establish and utilize gist representations at the encoding stage and impairments in processing on the basis of familiarity and recollection at the retrieval stage. Consequently, patients with AD have access to less information when making memory judgments. As a result, they become more susceptible to the effects of item fluency, which can be manipulated during the retrieval stage. Furthermore, with impaired source memory monitoring abilities, the capacity of AD patients to suppress memory illusions is compromised. Based on these findings, we propose that the study of false memories constitute a critical tool for elucidating the memory impairments involved in AD. Further explorations of these memory impairments will have practical significance for the diagnosis and treatment of AD in the future.     

基于CPLD控制的多路数据自动采集系统设计

分析了常用模数转换器的控制时序,并以AD0809为例,对由CPLD构建的多路采集控制电路的内部原理做了详细分析。给出了基于CPLD控制的多路数据自动采集系统设计方案,仿真验证本方案是可行的。     

基于AD7755的掌上型电能表现场校验仪的设计与实现

分析了现场校验仪的测量原理,给出了被校电度表的电压、电流、功率、电能表误差等参数的计算公式,并以CPU89C2051为控制计算核心,AD7755为测量核心,配以高精度钳型电流互感器和液晶显示模块,设计和实现了掌上型电能表现场校验仪系统.该系统可在不拆表、不断电的情况下,快速准确地测出被校电度表的电压、电流、功率、电能表误差等参数.     

降解除草剂阿特拉津的细菌聚生体的分离和鉴定

将阿特拉津降解速度快但降解不完全的混合菌群与阿特拉津降解完全但降解速度慢的Pseudomonas sp.ADP菌株混合,经过长期驯化培养,筛选到一个能完全降解阿特拉津而且降解速度快的细菌聚生体(bacterial consortium).聚生体含有两个菌株,其中一个菌株来自混合菌群,命名为AD25,另一个是ADP的突变株,命名为Pseudomonas sp.ADP-V.通过16S rRNA基因测序,AD25被鉴定为节杆菌(Arthrobacter sp.).PCR分析和降解实验表明,AD25含有trzN-atzBC基因,能将阿特拉津降解成氰尿酸;ADP-V含有atzDEF基因,是ADP菌株丢失atzABC基因以后的衍生菌,能使氰尿酸进一步降解.由AD25和ADP-V组成的细菌聚生体能快速和完全降解阿特拉津.ADP-V的atzD基因表达和酶动力学实验表明,由于它编码的氰尿酸水解酶(AtzD)的339位蛋氨酸(Met)突变为苏氨酸(Thr),导致酶活力降低从而引起在生长培养基中出现少量氰尿酸积累.     

基于AD9059的毫米波主被动复合探测器信号处理系统设计

针对毫米波主被动复合探测器主被动信号的差异问题,鉴于传统双AD芯片分别采样主被动信号,电路设计复杂,提出一种使用单一AD芯片的信号处理系统设计方法。使用AD9059采样主被动信号,并以TMS320VC5410为核心进行信号处理,实现对目标的测距和识别。该系统不仅设计简单,还具有干扰小、稳定性好、易小型化等优点。目标探测实验表明,该设计能够满足探测器的指标要求,能够很好的完成距离测量和目标识别等探测任务。     

基于LabVIEW的温度测试系统的研究

介绍了AD590温度传感器的特性和系统结构设计,重点阐述了虚拟仪器系统的构建和系统软件设计.利用虚拟仪器丰富的开发资源,基本不投入仪器设备及硬件,设计出具有较高的应用价值的传感器特性测试系统.并用软件进行滤波及归一化处理抑制采集噪声,提高系统的测试精度.     

DDS技术在步进电动机控制系统中的应用

直接数字频率合成(DDS)技术是目前广为应用的一项频率合成技术,它具有频率转换时间短、频率分辨率高、可编程等特点.正是利用直接数字频率合成技术产生步进电动机控制系统的步进脉冲.试验证明这种方法具有稳定、可靠、精度高等优点,非常适合那些对速度要求精确控制的系统.