水稻BpHi008A基因对褐飞虱取食应答及其编码产物在大肠杆菌中的高效表达

对抗虫相关蛋白BpH i008A进行了生物信息学分析,发现BpH i008A含有一个PKC磷酸化位点、一个CK2磷酸化位点、三个N-myristoylation site位点;另外,BpH i008A蛋白含有一跨膜螺旋,和位于细胞内的N-末端及伸向细胞外的C-末端.Northern分析证明褐飞虱的取食是维持BpHi008A基因的表达的必要条件.以pET28 a为载体构建了非融合表达的重组质粒,并且实现了BpHi008A基因在大肠杆菌菌株BL21(DE3)中的高效表达.     

竹红菌甲素与细胞色素C相互作用的同步荧光光谱研究

利用同步荧光光谱方法研究了在生理pH值条件下竹红菌甲素（HA）对细胞色素C分子构象的影响，同时探讨其作用机理．同步荧光光谱实验结果说明HA与细胞色素C之间的相互作用主要发生在HA和细胞色素C分子表面的氨基酸残基之间，最终这种作用使细胞色素C的构象发生变化．     

车辆导航中一种改进的路径优化算法

针对车辆定位与导航系统中的最优路径规划中存在的问题,研究了最短路径搜索算法的快速实现技术,提出了一种启发式快速最优路径规划算法.在分析经典迪杰斯特拉最短路径搜索算法和A*启发式搜索算法的基础上,利用双向A*算法和地图分层搜索技术减小搜索空间,采用二叉堆结构来实现路径计算过程中优先级队列的一系列操作,从而提高了算法的执行效率.仿真试验的结果证明了该算法的优异性能.     

Characterization of threerice CCoAOMT genes

Caffeoyl-Coenzyme A 3-O-Methyltransferase (CCoAOMT) is a key enzyme in lignin biosynthesis pathway. Three rice CCoAOMT genes were identified and designated as OsCOAI, OsCOA20 and OsCOA26. OsCOAI contains four exons and three introns, while the other two have three exons and two introns. The deduced amino acid sequences of these rice genes share a high identity (75.43%) with other plant CCoAOMT proteins and contain the CCoAOMT specific motifs. Phylogenetic analysis indicates that OsCOAI has the closest evolutionary relationship to maize CCoAOMT. In contrast, OsCOA20 and OsCOA26 belong to another clade.Northern blot analyses and in situ hybridization studies indicate that the three rice CCoAOMT genes are highly expressed in developing sclerenchyma cells and vessel bundles of young leaves, suggesting that they are probably involved in constitutive lignification.     

Effects of 2,3,7,8-TCDD and benzo[a]pyrene on modulating vitellogenin expression in primary culture of crucian carp （Carassius auratus） hepatocytes

Vitellogenin (Vtg) is the precursor of yolk protein. Its expression and secretion are estrogen-regulated and are crucial for oocyte maturation. An in ritro xenoestrogen screening model was established by measuring Vtg induction in cultured primary hepatocytes from crucian carp. Vtg production was detected by biotin-avidin sandwich ELISA method while Vtg and cytochrome P4501A1 (CYP1A1) mRNA induction were measured by semi- quantitative PCR-primer dropping technique. Vtg and Vtg mRNA were dose-dependently induced by diethylstilbestrol (DES, 0.2-200 ng/mL) in hepatocytes of crucian carp. Co-treatment of the DES-induced hepatocytes with either 2,3,7,8-TCDD (TCDD, 0.1-4 pg/mL) or benzo[a]pyrene (B[a]P, 5-1000 ng/mL) resulted in a reduction of Vtg production and an increment of CYP1A1 mRNA expression both in a dose dependent manner, indicating the anti-estrogenic effects of the compounds. However, at lower tested concentrations, TCDD (0.1, 0.2 pg/mL), B[a]P (5 ng/mL)seemed to have a potentiating effect on Vtg expression and secretion, although by their own these compounds had no observable estrogenic effect on Vtg induction. Tamoxifen (a selective estrogen receptor modulators, 1 nmol/L-1μmol/L),and β-naphtho-flavone (β-NF, an aryl hydrocarbon receptor inducing compounds, 2.5-1000 ng/mL) also were employed to study the possible interactions in DES-induced Vtg expression. In co-treatment of the DES-induced hepatocytes with β-NF or tamoxifen, the decrease in Vtg production did parallel induction of CYP1A1 for β-NF, but tamoxifen inhibited Vtg induction did not parallel induced CYP1A1 expression in all test concentrations. On the contrary, it was found that in co-treatment of the TCDD-induced hepatocytes with DES, TCDD induced CYP1A1 mRNA production was inhibited by DES also. These results implicated a possible cross talk between estrogen receptor- and aryl hydrocarbon receptor-mediated pathways in the hepatocytes.     

两个新的海洋神经酰胺

从南海海绵Spongia sp.中分离得到两个新的神经酰胺,spongiamine A(1)和sprongiamine B(2),通过IR,1D NMR,2D NMR,MS,MSn等波谱方法,确定了它们的结构分别为N-(2＇-羟基-正-二十四碳酰基)-1,3,4-三羟基-正-十五烷-鞘胺醇(1)和N-(2＇-羟基-正-二十四碳酰基)-正-二十碳-4(E)烯-鞘胺醇(2).     

一种新型天蚕素杂合肽AD和aFGF突变体融合蛋白S的构建及表达

目的：构建和表达一种带有凝血酶切割位点的天蚕素杂合肽AD和人酸性成纤维细胞生长因子(acidic fibroblast growth factor，aFGF)突变体的融合蛋白S，在创伤皮肤表面应用时能自动裂解成有抗感染功能的天蚕素杂合肽AD和皮肤修复功能的aFGF突变体。方法：利用P(R技术分别扩增出带有凝血酶切割位点的天蚕素杂合肽AD基因和aFGF突变体基因。再利用两作为模板，用PCR方法扩增出编码融合蛋白S的DNA。将该DNA片段插入到载体pPICZaA中，并利用电转法将质粒转入毕赤酵母smd1168中，Zeocin抗性筛选重组转化子。甲醇诱导72h，SDS-PAGE电泳检测融合蛋白S的表达，并用Western-blot杂交检测融合蛋白S的免疫原性。结果：筛选出的重组转化株在甲醇诱导后能表达相对分子质量约为19000的融合蛋白S，而该蛋白能与aFGF抗体产生免疫反应。结论：用PCR方法获得编码含凝血酶切割位点的天蚕素杂合肽AD和aFGF突变体的融合蛋白SDNA，并在毕赤酵母中实现了表达。     

Kelletinin I and kelletinin A from the marine molluscBuccinulum corneum are inhibitors of eukaryotic DNA polymeraseα

Summary The inhibitory effect of esters of p-hydroxybenzoic acid (kelletinins I and A), extracted from the marine gastropodBuccinulum corneum, have been tested on eukaryotic and prokaryotic enzymes of DNA metabolism such as DNA polymerases and , DNA polymerase I, Exo III, pancreatic DNAse I, micrococcal DNAse andE. coli RNA polymerase. Kelletinin I and kelletinin A inhibit preferentially DNA polymerase. The inhibitory effect of kelletinin I involves the hydroxyl group of p-hydroxybenzoic acid.     

HLA FEDEP及VV＆A的工作流技术研究

HLA 仿真系统的 FEDEP 及 VV&A 过程比较复杂,实际工作中需要耗费大量的人力物力。为了提供一种实现 FEDEP 及 VV&A 过程自动化的方法,在分析了 FEDEP 与 VV&A 过程实质的基础上,介绍了 FlowMark 工作流模型并基于其阐述了 FEDEP 与 VV&A 工作流元素的实现方法,介绍了工作流自动化所需的基本服务和工具,分析了实现工作流引擎的关键技术,并介绍了实现 FEDEP与 VV&A 工作流自动化的原理及程序界面,最后对研究做出了结论。应用工作流技术可以有效地实现 FEDEP 及 VV&A 过程的自动化,拥有良好的发展前景。     

数字给定式稳压电源的设计

本文根据串联型稳压电源理论,结合数字电子技术,采用模 数结合的方法,讨论了数字式稳压电源的设计方法,并对部分电路进行了实例分析。