马铃薯病毒一步法RT-PCR诊断研究

运用一步法RT-PCR对马铃薯和烟草中的马铃薯X病毒、马铃薯Y病毒和马铃薯卷叶病毒进行了检测,实验表明:对酚-SDS方法提取的病毒核酸进行扩增,一步法RT-PCR检测到的扩增产物的灵敏度较两步法RT-PCR的高100倍左右.两种提取方法对比,用一步法RT-PCR进行扩增,检测到的PEG法扩增产物的灵敏度较酚-SDS法扩增产物的灵敏度高3倍.建立了快速、简便、灵敏度高、特异性强的马铃薯病毒一步法RT-PCR检测技术.     

ERwin在智能考试系统中的应用

介绍了ERwin的特点，以一个具体的实例应用阐述了如何使用ERwin在数据库设计阶段建立逻辑模型和物理模型，并用物理模型在数据库服务器上产生模型实例等技术问题，并给出了使用ERwin建立的智能考试系统的逻辑模型和物理模型．对于想高效、快速建立实体一关系(DR)模型的数据库应用程序的开发者来说，有一定的参考价值和借鉴意义．     

基于嵌入式农田信息采集系统的研究

基于嵌入式系统的农业数据采集系统采用先进的无线通信技术、GPS定位技术和嵌入式技术来解决农业数据的远程采集问题.论文以嵌入式系统为核心,提出和分析了实现系统的关键技术.采用无线通信GPRS网络将现场采集到的有关经纬度、高程、时间以及农田环境数据等信息进行分析和处理,并传送到远程管理中心,为农业生产提供决策支持.它能够解决农业的远程数据采集和传输问题.     

乙烯副产C9燃料油萃取脱硫萃取剂

对C9燃料油溶剂萃取脱硫的萃取剂进行研究,选择①乙腈、②N-甲基吡咯烷酮(NMP)、③环丁砜与二甲基亚砜、④二乙二醇、三乙二醇和甲醇、乙醇、乙二醇⑤糠醛5组萃取剂对C9燃料油进行萃取实验,从中筛选出较佳的萃取剂.分别研究了含水量、油剂质量比对萃取脱硫率和收率的影响,确定适宜的萃取条件,为以后的工业化生产提供基础数据.在此基础上,考察不同萃取剂复配的脱硫效果.实验结果表明,含水10%~14%的乙腈溶液或含水15%~17%的NMP溶液是C9燃料油较为适宜的萃取溶剂,其与二乙二醇复配可以进一步提高脱硫效果.     

A nonparametric method for asymmetrically extending signal extraction filters

Two important problems in the X‐11 seasonal adjustment methodology are the construction of standard errors and the handling of the boundaries. We adapt the ‘implied model approach’ of Kaiser and Maravall to achieve both objectives in a nonparametric fashion. The frequency response function of an X‐11 linear filter is used, together with the periodogram of the differenced data, to define spectral density estimates for signal and noise. These spectra are then used to define a matrix smoother, which in turn generates an estimate of the signal that is linear in the data. Estimates of the signal are provided at all time points in the sample, and the associated time‐varying signal extraction mean squared errors are a by‐product of the matrix smoother theory. After explaining our method, it is applied to popular nonparametric filters such as the Hodrick–Prescott (HP), the Henderson trend, and ideal low‐pass and band‐pass filters, as well as X‐11 seasonal adjustment, trend, and irregular filters. Finally, we illustrate the method on several time series and provide comparisons with X‐12‐ARIMA seasonal adjustments. Copyright © 2010 John Wiley & Sons, Ltd.     

DanHong Injection inhibits the development of primary abdominal aortic aneurysms in apoE knockout mice

Clinical observations indicate that DanHong Injection （DHI） can increase blood flow and reduce various syndromes in patients with cardiovascular disease. How- ever, it still needs to define the function of DHI and the involved mechanisms in details, such as the protective effect on the development of primary abdominal aortic aneurysms （AAAs）. In this study, we determined whether DHI is able to inhibit AAA in apoE knockout （apoE-/-） mice. Thirty apoE-/- male mice on high-fat diet （0.5 % cholesterol, 21% fat） were randomly divided into two groups and received i.p. injection of saline （100 μL/day） and DHI （100 μL/day）, respectively, for 16 weeks. At the end of experiment, we determined the development of atherosclerosis in en face aorta and aneurysms,pathological morphology of arterial wall, and serum lipid levels. We also determined the expression of monocyte chemoattractant protein-1 （MCP-1）, MMP-2, and MMP-9 mRNA in aortic wall using real-time RT-PCR. Our results indicated that high-fat diet induced the development of AAAs in apoE-/- mice, but the induction was totally blocked by DHI （P 〈 0.01）. The result of staining of abdominal aortic cross sections showed that DHI main- tained the collagen content in arterial wall, thereby pre- venting the animals from the development of AAA. Although DHI had little effect on serum total- and LDL- cholesterol levels, it reduced the expression of MCP-1, MMP-2, and MMP-9 mRNA in aortic wall （P 〈 0.01）. Taken together, our study suggests that DHI can inhibit the high-fat diet-induced AAA formation. The inhibitory effects may be related to the maintenance of the collagen content and inhibition of expression of AAA-related genes. Our study may suggest a new application of DHI in clinics.     

基于改进Sobel算法的焊缝X射线图像气孔识别方法

提出一种具有自适应能力的焊缝X射线图像气孔检测方法.通过局部动态阈值分割法设计了多方向的焊缝X射线图像模板,采用Sobel算法对复杂背景下的焊缝进行边缘检测和区域标记,并在标记区域对原始图像x和y方向的灰度梯度进行分析,以增强小气孔和粘连气孔的识别能力.检测应用效果表明,所提出的方法具有良好的自适应能力且准确快速,能够有效地克服人工评片中产生的漏判与误判等缺点,并且能够存储和查询检测数据.     

Waugh结构(NH_4)_6[MnMo_9O_(32)]·8H_2O催化合成乙酸异戊酯

以Waugh结构钼锰杂多酸盐作为催化剂对乙酸异戊酯的合成进行了催化研究.确定了最佳反应条件:醇酸摩尔比为1.646∶1,酸化催化剂用量为0.2 g,带水剂为3 mL甲苯,反应时间63 min左右,选择性100%,转化率达82.70%.     

污水处理厂出水维甲酸X受体干扰效应研究

应用重组维甲酸X受体(RXR)基因酵母筛选南方某污水处理厂进水、不同工艺出水的维甲酸干扰活性;结合大鼠肝均浆(S9)体外代谢方法,检测样品间接维甲酸干扰活性.结果表明:进水、出水样品均不能诱导RXR介导酶活性,但在高富集倍数下,样品与空白对照相比显著抑制9-顺维甲酸诱导酶活性,表现出维甲酸拮抗效应;在添加S9体外代谢活化后,所有样品均检出类维甲酸干扰活性,部分样品还检测出抗维甲酸活性,表明城市污水样品中存在大量具有类/抗维甲酸干扰活性的化合物.研究结果证实,现行处理工艺对类/抗维甲酸干扰物有一定的去除效果;重组基因酵母结合S9体外代谢活化体系可用于水环境样品类/抗维甲酸干扰效应的快速筛选.     

Angular dependent NEXAFS study of the molecular orientation of PTCDA multilayers on Au （111） surface

The molecular orientation of perylene-3,4,9,10-tetracarboxylic acid dianhydride (PTCDA) multilayers adsorbed on Au (111) surface has been investigated using angular dependent O K-edge near edge X-ray absorption fine structure (NEXAFS) spectroscopy. The significant angular dependence of important resonant structures (π* and σ*) reveals that PTCDA molecules adopt an ordered geometry on the substrate surface. The average tilt angle of the PTCDA molecular planes is 27°±10° from the Au (111) surface.