新型液晶材料主链聚缩醛螺胞二醚的合成

在催化剂的作用下利用苯甲醛与季戊四醇,合成了聚缩醛螺胞二醚的模型化合物3,9 二苯基 2,4,8,10 四氧杂螺[5·5] 十一烷,并在此基础上利用1,4 苯二甲醛与季戊四醇在催化剂的作用下成功地合成了标题化合物.考察了催化剂、反应物的摩尔比、带水剂、反应温度等对反应的影响,利用FTIR、HNMR对模型化合物及标题化合物的结构进行了表征,标题产品的最高收率可达8500以上.     

如何解除PCR反应中的抑制现象

多种因素均能影响PCR反应的顺利进行，尤其是菌体中的各种杂蛋白以及在模板提取过程中混入的各种化学试剂，对TaqDNA聚合酶均有很强的抑制作用，本文以扩增细菌的16SrDNA为例，说明了这些成分对PCR的抑制，同时提出了克服不利因素的方法。     

AB-8大孔吸附树脂分离长瓣金莲花总黄酮

研究了AB-8大孔吸附树脂对金莲花总黄酮的吸附和解吸特性，并比较了AB-8树脂与聚酰胺对金莲花总黄酮的纯化能力，结果表明，经AB-8树脂纯化的总黄酮纯度为38.9%，比粗提物的总黄酮纯度提高了2.88倍，且其纯化能力与聚酰胺相当。     

2-苯基-8-羟基喹啉锂和8-羟基喹啉锂的合成及荧光性质

合成了一种新型的2-苯基-8-羟基喹啉配合物.利用元素分析、红外吸收光谱等方法对配合物的组成进行了分析,分析结果表明:2-苯基-8-羟基喹啉可与锂离子以1:1的比例形成配合物;对比研究了2-苯基-8-羟基喹啉锂和8-羟基喹啉锂的荧光性质,前者的荧光发射峰位置较后者红移了37 nm.从而说明苯环的取代增加了有机配体π-π共轭的程度,使HOMO-LUMO之间能隙变小,引起发射峰红移.     

辛可芬与8-羟基喹啉稀土三元配合物的合成及有关烧伤的药学性质研究

合成了3种辛可芬(2-苯基喹啉-4-甲酸,HL)与8-羟基喹啉(HQ)的稀土(Ln)三元配合物,通过元素分析,摩尔电导,UV,IR,1HNMR等的研究,确定配合物的化学组成为Ln(HQ)(L)3·H2O(Ln=Ce(Ⅲ),Nd(Ⅲ),Sm(Ⅲ)),观察了它们的抗炎作用、半数致死量(LD50)和在霜剂中的配伍稳定性.     

Dual regulatory effects of resveratrol on activation of NF-κB and cell proliferation in human embryonal kidney 293 cells

Resveratrol (3,4‘,5-trihydroxystilbene, Res), a naturally occurring polyphenol, exhibits antioxidant, antiinflammatory, potential chemopreventive and chemotherapeutic properties in preclinical studies. To further understand its potential clinical efficacy and safety, effect of Res at 10^-9-10^-4 mol/L on human embryonal kidney (HEK293) cell proliferation and its potential mechanism were investigated in present study. Cell viability was detected by using trypan blue dye exclusion method. Cell cycle and apoptosis were analyzed by flow cytometry with propidium iodide stain.Activation of nuclear factor-κB (NF-κB) was determined by luciferase reporter gene assay using stably transfected HEK293/κB-luc cells. Secretion of human interleukin-8(hlL-8) was measured by ELISA. Our results show that HEK293 cell proliferation was significantly stimulated by 10^-7 mol/L Res after treatment for 48 hours, or by 10^-8-10^-7mol/L Res combinated with 10 ng/mL TNFα for 24 h, but was suppressed by 10^-4 mol/L Res with or without TNFα. Both endogenous and TNFα-induced NF-κB activation were downregulated by Res at 10^-7 mol/L, but were upregulated at 10^-4 mol/L. With 10^-4 mol/L Res, the content of secreted IL-8 was increased, and apoptosis rate was increased from lessthan 5 % to 10%, together with significant cell-cycle arrest in S phase. TNFα has coordinative effects with Res on HEK293 cell apoptosis, cell-cycle arrest and IL-8 secretion. These results indicate that Res promotes cell proliferation at low concentration through down-regulation of NF-κB activation in HEK293, but suppresses its growth at high concentration through up-regulation of NF-κB activation, increasing IL-8 and cell-cycle arrest. As resveratrol has dual regulatory effect on cell proliferation in vitro, comprehensive evaluation of its potential clinical utility is needed.     

环戊二烯基羰基铁乙酸烷基酯的质谱研究

环戊二烯基羰基铁衍生物[C_5H_5Fe(CO)_2CH_2COOR]的质谱表明该衍生物可以从两条途径碎裂,最后降解为裸露的铁正离子(Fe~+),且C_5H_5Fe~+离子还可以脱去C_2H_5形成C_3H_3Fe~+离子。从铁配位化合物的质谱中观察到的足够亚稳离子确立了从分子离子到裸露的铁离子的降解途径。     

有色噪声观测量的逐次静态滤波

配置的函数模型中除包含承机部分外，还包含非随机部分，给出了有色噪声情况下的逐步配置理论与方法。根据广义最小二乘原理推导出有色噪声情况下逐步配置的方法，即通过改化各观测方程，将有色噪声观测值改化成白噪声的观测值，然后用白噪声逐步配置公式进行计算，利用有色噪声观测值的逐步配置的理论，可消去观测方程的相关性，减少计算时间和内存，使编写计算程序简单化。提出的逐步配置理论是相关观测值逐次间接平差理论的推广，既相关观测值逐次间接平差、白噪声情况下静态逐步配置和白噪声所驱动的有色噪声的静态逐步配置都是有色噪声观测值的逐步配置的特例。     

固—液萃取法研究8-羟基喹啉对稀土元素的萃取性能

本文在用比重瓶法测定切片石蜡(PC)比重并导出其比重与温度关系的基础上,通过对RE~(3 )—HQ-PC体系的研究。结果表明:将4 gPC分两次萃取RE~(3 ),萃取率可达100％,与液一液萃取结论类似,最佳萃取酸度为pH8.00～10.00;组成研究结果表明,萃入石蜡中RE与HQ组成比为1:1,说明与液—液萃取机理不同。     

含钛炉渣碱度表示方法的比较

从脱硫的角度对３各实用的含钛炉渣碱表示方法作了比较。光学碱度在炉渣组分变化很大的范围内，均能较满意地揭示含钛炉渣的冶金性能。在１５００℃和１４５０℃下由气／渣和金／渣平衡数据，分别求得硫容量与光学碱度的相互关系。