Integrase, LEDGF/p75 and HIV replication

HIV integrates a DNA copy of its genome into a host cell chromosome in each replication cycle. The essential DNA cleaving and joining chemistry of integration is known, but there is less understanding of the process as it occurs in a cell, where two complex and dynamic macromolecular entities are joined: the viral pre-integration complex and chromatin. Among implicated cellular factors, much recent attention has coalesced around LEDGF/p75, a nuclear protein that may act as a chromatin docking factor or receptor for lentiviral pre-integration complexes. LEDGF/p75 tethers HIV integrase to chromatin, protects it from degradation, and strongly influences the genome-wide pattern of HIV integration. Depleting the protein from cells and/or over-expressing its integrase-binding domain blocks viral replication. Current goals are to establish the underlying mechanisms and to determine whether this knowledge can be exploited for antiviral therapy or for targeting lentiviral vector integration in human gene therapy. Received 25 November 2007; received after revision 7 January 2008; accepted 10 January 2008     

U75V高速轨钢脱碳的影响因素分析

在不同炉内气氛、加热温度、停留时间以及是否采用保护涂料等条件下,对U75V高速轨钢脱碳的影响因素进行分析。结果表明,正常轧制时,应尽量避免预热段为氧化性气氛,在预热段空气消耗系数为1.2、均热段空气消耗系数为0.8时,钢坯的脱碳层深度最浅;采用保护涂料方式可使钢坯氧化烧损量减少20%～30%,脱碳层深度减少29.7%。     

基于RS485总线技术的PC机与单片机多机通讯设计

采用SN75LBC184来实现RS232C与RS485串行口的转换,从而使PC机具备主从式多机通讯功能,实现对多个单片机组成数据采集终端的通讯与管理.约定一种简单和有效的串口通讯协议,根据这种通讯协议设计出单片机与PC机串口通讯程序.     

U75V 60kg/m重轨欠速冷却后的组织及性能

U75V 60kg/m重轨试样加热至900℃,模拟重轨万能轧制成品道次出口温度;随后,空冷25 s,模拟重轨从成品道次出口至冷床的温降;最后,将试样放入喷风通道对轨头表面和两个侧面进行欠速冷却.通过实验获得了U75V 60kg/m重轨最佳冷却工艺参数:喷嘴离轨头表面距离15mm,喷风压力0.26MPa,喷风时间80 s,风温28℃.在此欠速冷却条件下,轨头硬化层为对称帽形,轨头中心部位硬化层厚度大于24mm;组织为细珠光体,其平均片层间距比冷却前减小约50 nm;在轨头上圆角部位强度大于1 300 MPa,延伸率大于13%,分别比冷却前提高20%和60%以上.     

基于单片机矿用电机车牵引控制器的研制

为了实现矿用电机车交流传动的高性能控制运行,提高电机车运行的可靠性,以代替传统的矿用电机车直流调阻调速和直流斩波调速,实现节能降耗。本文研制设计了基于16位单片微机SPMC75F2413A的矿用电机车交流变频调速牵引控制器,给出了牵引控制器三相SPWM波形发生器的硬件和软件的实现方案和电路结构。实验结果证明,该牵引控制器能够满足矿用电机车交流变频调速运行的要求。