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11.
根据CyanoBase提供的鱼腥藻PCC7120 FurC基因(alr0957)序列信息设计特异性引物,用降落PCR法从染色体DNA中扩增得约450 bp目的片段.运用TA克隆将其连接到pMD18-T载体上,经筛选测序获得阳性重组质粒.再经过双酶切、纯化FurC基因,连接原核表达载体pET-28a(+),并转化表达菌株BL21和IPTG诱导表达.经测序鉴定的阳性菌株中的FurC,运用SDS-PAGE检测重组蛋白,并利用镍柱层析法纯化目的蛋白.由藻细胞密度、叶绿素a含量、可溶性糖含量等改变探讨不同Fe3+浓度对藻类生长的影响.结果表明:在37℃经1 mmol/L IPTG诱导18 h,成功表达了分子量约为19000的融合蛋白.小于0.5mg/L Fe3+促进藻生长,大于0.9 mg/L Fe3+抑制藻生长,最适藻生长Fe3+浓度为0.50.9 mg/L. 相似文献
12.
环境因子对转基因鱼腥藻7120生长的影响 总被引:1,自引:0,他引:1
研究了培养瓶规格、光照时间、光质以及温度对转基因鱼腥藻7120生长的影响,结果表明:在5%接种量下,培养瓶规格越小,越有利于藻体的生长;藻体的生长以每天18h的光暗交替的光照时间最好;单色光对藻体的生长以蓝光效果最好;藻体生长的最佳温度为25~30℃。 相似文献
13.
XU Wen-liang LIU Yong-ding ZHANG Cheng-cai LI Juan . College of Life Sciences Central China Normal University Wuhan Hubei China . Institute of Hydrobiology Chinese Academy of Sciences Wuhan Hubei China . College of Life Sciences Technology Huazhong Agricultural University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2004,9(4)
0 IntroductionIronisthefourthmostabundantelementbyweightintheearth’scrust.Itisrequiredfornearlyallorganisms.Itinvolvedinawidevarietyofbiochemicalprocessesbothasacofactorandcatalyst.Theseprocessesincludephotosynthesis,chlorophyllproduction ,respiration,nitrateandnitritereduction,nitrogenfixation ,andotherbi ologicaloxidations.However,itisanelementwithalowbiologicalavailabilityduetoitspoorsolubilityinoxygenicaqueoussolution .Livingor ganismsareconstantlyfacedtotheproblemofhowtoovercomethelows… 相似文献
14.
xuwen-liang LIUYong-ding ZHANGCheng-cai LIJuan 《武汉大学学报:自然科学英文版》2004,9(4):498-502
The fecC gene encoding a putative iron (Ⅲ) dicitrate transporte rwas cloned from nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, and inactivated. The mutant grows normally in medium with NO3^- , NH1^- or without combined nitrogen. But in iron-deficient medium, the mutant grows slowly. Photosynthetic properties were compared between the mutant and the wildtype strain, the content of photosynthetic pigments in the mutant is lower than that of the wild-type. The results of RT-PCR experiments show that the fecC gene is expressed under iron-deficient conditions, but is not expressed under iron-replete conditions. These results revealed that fecC gene product is required for optimal growth under iron-deficient conditions in Anabaena sp. PCC 7120. 相似文献
15.
转基因鱼腥藻7120光异养生长的研究 总被引:1,自引:0,他引:1
研究了不同有机碳源对转基因鱼腥藻7120生长的影响,在此基础上进行了光异养条件的优化,并研究了外源添加剂L-精氨酸、生长激素2,4-D与KT对藻细胞生长的影响。结果表明:蔗糖和葡萄糖能促进藻体的生长;最优碳氮源组合为葡萄糖6g/L、NaNO31.5g/L,经高压灭菌;L-精氨酸不支持藻体的光异养生长;一定含量的2,4-D与KT能促进藻体在光异养条件下的后期生长。 相似文献
16.
The effect of iron deficiency on heterocyst differentiation and some physiological properties of the filamentous cyanobacteriumAnabaena sp. PCC 7120 was investigated. Under moderate iron limitation conditions, achieved by addition of iron chelator 2, 2′-Dipyridyl
(<80 μmol/L) led to delayed heterocyst differentiation, no heterocyst differentiation was observed under severe iron limitation
conditions, when the concentration of 2,2′-Dipyridyl in the medium was more than 100 μmol/L. it seemed that there are certain
iron-regulated genes or operons whose function is to control heterocyst development. In addition, iron deficiency impaired
the growth. Low iron cells had a decrease in the quantities of pigment content (chlorophyll and phycocyanin content), the
whole cellin vivo absorbance spectra confirmed the decrease, the protein electrophoretic profiles revealed that iron-deficient cells had less
protein bands, with the increase of 2,2′-Dipyridyl, the protein bands was more and more less. And differently, iron deficiency
also caused an increase of ROS (Reactive Oxygen Species) and SOD activity, it suggests that iron deficiency led to oxidative
stress, which generally occured under high-iron conditions.
Foundation item: Supported by the National Natural Sciences Foundation of China (30070154), the Frontier Science Projects Programme of the
Institute of Hydrobiology, the Chinese Academy of Sciences (220316), State Key Project on Cyanobacterial Bloom Control in
Lake Danchi (K9905-35-01)
Biography: Xu Wenllang (1974-), male, Ph. D. research direction: molecular genetics of eyanobacteria. 相似文献